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BioNanoScience

, Volume 4, Issue 4, pp 338–348 | Cite as

Filtration of Nematodes using an Integrated Microcapillary System

  • András J. Laki
  • Kristóf Iván
  • Éva Fok
  • Pierluigi Civera
Article
  • 103 Downloads

Abstract

We demonstrate a novel microfluidic device to detect living parasites from native blood using monolithic polydimethylsiloxane (PDMS) structures. Several blood-borne and tick-borne parasites such as dirofilariosis can be investigated by this microcapillary system. Inside this microfluidic device, a special flow-through separator structure has been implemented which contains a cylindrical active zone, where the microfilariae or other few micron-size pathogens remain trapped. The center region is partially surrounded by microcapillaries which are positioned along an isobaric line to aid in filtration efficiency through pressure equalization. The developed test can be optimized for a specific nematode or pathogen detection by adjusting the capillary width.

Keywords

Veterinarian diagnostics Blood-borne parasitosis Microfluidic filtration device 

Notes

Acknowledgment

This work was supported by Hungarian National Development Agency grants TÁMOP-4.2.1.B-11/2/KMR-2011-0002 and TÁMOP-4.2.2/B-10/1-2010-0014. We would like to extend our gratitude to Dr. Ibolya Kocsis (1st Department of Internal Medicine, Semmelweis University, Budapest), Péter Fürjes (MEMS Laboratory, Research Center for Natural Sciences, Hungarian Academy of Sciences, Budapest), and Olga Jacsó for their kind help.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • András J. Laki
    • 1
    • 2
  • Kristóf Iván
    • 3
  • Éva Fok
    • 4
  • Pierluigi Civera
    • 2
  1. 1.Faculty of Information Technology and BionicsPázmány Péter Catholic UniversityBudapestHungary
  2. 2.Department of Electronics and TelecommunicationsPolytechnic University of TurinTurinItaly
  3. 3.Faculty of Information Technology and BionicsPázmány Péter Catholic UniversityBudapestHungary
  4. 4.Department of Parasitology and Zoology, Faculty of Veterinary ScienceSzent István UniversityBudapestHungary

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