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Synthesis and Characterization of Silica-Coated Silver Nanoprobe for Paraoxon pesticide Detection

BioNanoScience volume 4, pages 149–156 (2014)Cite this article

Abstract

Metal enhanced fluorescence assay-based sensitive detection of a neurotoxic organophosphates pesticide, Paraoxon, is reported. The experimental approach involves the bio-interfacing of organophosphorus hydrolase with a high quantum yield fluorophore, pyranine (8-hydroxyl pyrene-1,3,6-trisulfonic acid trisodium salt), followed by the conjugation of the OPH-pyranine derivative with silica-coated silver nanoparticles (Ag NPs). The above bio-nanoprobe was used for the analysis of the organophosphate pesticide, wherein the induced hydrolysis of the pesticide cause the decrease in pH in the vicinity. An excitation light of 460 nm wavelength was used to monitor the changes in the resulting emission (at 510 nm) with respect to the changing pesticide concentrations (1–100 ppb). The introduction of the silica-coated AgNPs into the nanoprobe system was observed to deliver metal enhanced effect, leading to almost10-fold intensification of the fluorescence signal. The enhanced fluorescence assay format offers linear detection of Paraoxon in the concentration range of 1–100 ng mL−1 with the limit of detection 1 ng mL−1 (1 ppb).

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Acknowledgments

The authors gratefully acknowledge the support of the director, CSIR-CSIO, Chandigarh. One of the authors (Satish K Tuteja) thanks CSIR, New Delhi, India for granting senior research fellowship.

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Authors and Affiliations

  1. Academy of Innovative and Scientific Research (AcSIR)-CSIO, Chandigarh, 160030, India

    Satish K. Tuteja, Manil Kukkar & Akash Deep

  2. CSIR-Central Scientific Instruments Organization, Chandigarh, 160030, India

    Satish K. Tuteja

  3. Sri Guru Granth Sahib World University (SGGSWU), Fatehgarh, 140406, India

    Manil Kukkar, Parveen Kumar, A. K. Paul & Akash Deep

Authors
  1. Satish K. Tuteja
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  2. Manil Kukkar
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  4. A. K. Paul
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  5. Akash Deep
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Correspondence to Satish K. Tuteja or Akash Deep.

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Tuteja, S.K., Kukkar, M., Kumar, P. et al. Synthesis and Characterization of Silica-Coated Silver Nanoprobe for Paraoxon pesticide Detection. BioNanoSci. 4, 149–156 (2014). https://doi.org/10.1007/s12668-014-0129-6

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