, Volume 2, Issue 4, pp 316–321 | Cite as

Extracellular Biosynthesis of Silver Nanoparticles Using Fungi Penicillium diversum and Their Antimicrobial Activity Studies

  • Sharanabasava V. Ganachari
  • Ravishankar Bhat
  • Raghunandan Deshpande
  • A. VenkataramanEmail author


In this present investigation, we report a simple, cost-effective, and eco-friendly method of synthesizing colloidal silver nanoparticles by using fungi Penicillium diversum. UV–visible spectroscopy studies were carried out to quantify the formation of silver nanoparticles. The X-ray diffraction pattern suggests the crystallinity of silver nanoparticles. Atomic force microscopy and transmission electron microscopy images show that the silver nanoparticles are polydispersed and are in a size range of 5 to 45 nm with an average size of 20 nm. From the Fourier transform infrared spectroscopy, we presume that the reductase enzyme present in the fungal extract may be responsible for the reduction and stabilization of the silver nanoparticles. The resultant silver nanoparticles showed effective antimicrobial activity against Escherichia coli, Salmonella typhi, Vibrio cholerae, and the clinical isolate of Paratyphia.


Extracellular biosynthesis Silver nanoparticles Penicillium diversum Morphological study Antimicrobial activity 



The authors are grateful to UGC, Major Research Project (F. No. 33-307/2007 (SR), DAE-BRNS Project (No.2009/34/14/BRNS), and VGST (SMYSR-D38/7), Bangalore, for financial assistance. We also acknowledge help from SAIF, IIT Mumbai for TEM measurements, and Biogenics, Hubli, for antimicrobial studies. We thank Shri. Jagannathrao M. Deshpande, father of author Raghunandan Deshpande, for editing work.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Sharanabasava V. Ganachari
    • 1
  • Ravishankar Bhat
    • 1
  • Raghunandan Deshpande
    • 2
  • A. Venkataraman
    • 1
    Email author
  1. 1.Materials Chemistry Laboratory, Department of Materials ScienceGulbarga UniversityGulbargaIndia
  2. 2.H.K.E.’s Matoshree Taradevi Rampure Institute of Pharmaceutical SciencesGulbargaIndia

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