Abstract
Highly dispersed nanoporous Au rods have been prepared from arc melted Ag70Au30 alloy using site-selective electrochemical potential controlled dealloying method followed by Au anodizing process. The microstructure studies using scanning electron microscope and atomic force microscope indicate that selective removal of Ag from the Ag70Au30, precursor results in the formation of bi-continuous three-dimensional network structures with uniform pores and Au ligaments diameter of 6 nm. The noticeable features present in the optical studies of highly dispersed nanoporous Au rods using UV—Vis spectroscopy are two absorption bands peaking at 477 and 546 nm with overlapping of these bands at 520 nm. The antimicrobial testing by Agar well diffusion method reveals that the dispersed porous Au rods have greater inhibitory effect against both Gram-positive and Gram-negative bacterial strains. The results obtained provide opportunities to design and manipulate nanoporous Au rods at atomic level to achieve antimicrobial efficacy for biomedical applications.
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Erkin Seker, Yevgeny Berdichevsky, Kevin J. Staley, Martin L. Yarmush, Microfabrication-Compatible Nanoporous Gold Foams as Biomaterials for Drug Delivery, Adv. Healthcare Mater. 1, 172–176.(2012)
V. Garcia-Gradilla, S. Sattayasamitsathit, F. Soto, F. Kuralay,C. Yardımcı, D. Wiitala M. Galarnyk, J. Wang, Ultrasound-propelled Nanoporous Gold Wire for Efficient Drug Loading and Release, Small 10 4154 – 4159 (2014).
H. Min, N. Sullivan, D. Allarab, S. Tadigadapa, Nanoporous Gold: A high sensitivity and specificity biosensing substrate, Procedia Engineering, 25 1469–1472 (201l).
P. Daggumati, O. Kurtulus, C. A. R. Chapman, D. Dimlioglu, E. Seker, Microfabrication of Nanoporous Gold Patterns for Cell-material Interaction Studies, JOVE Journal of Visualized Experiments 77: e50678 (1–7) (2013).
J. Erlebacher, M. J. Aziz, A. Karma, N. Dimitrov, K. Sieradzki, Evolution of nanoporosity in de-alloying, Nature 410, 450 - 453 (2002).
J. Weissmüller, R.C. Newman, H.-J. Jin, A.M. Hodge, J.W. Kysar, Nanoporous Metals by Alloy Corrosion: Formation and Mechanical Properties, MRS Bull.. 34, 577 - 586 (2009).
J. Biener, A. Wittstock, L. A. Zepeda-Ruiz, M. M. Biener, V. Zielasek, D. Kramer, R. N. Viswanath, J. Weissmüller, M. Bäumer, A. V. Hamza, Surface-chemistry-driven actuation in nanoporous gold, Nat. Mater. 8, 47–51 (2009).
R. N. Viswanath, V. A. Chirayath, R. Rajaraman, G. Amarendra, C. S. Sundar, Ligament coarsening in nanoporous gold: Insights from positron annihilation studyApplied Physics Letters 102, 253101(1-4) (2013).
N. Miyazawa, M. Hakamada, M. Mabuchi, Antimicrobial mechanisms due to hyperpolarization induced by nanoporous Au, Scientific Reports,8, 3870 (1-5) (2018).
K. Gold, B. Slay, M. Knackstedt, Akhilesh K. Gaharwar, Antimicrobial Activity of Metal and Metal‐Oxide Based Nanoparticles, Adv. Therap.1, 1700033 (1-15) (2018).
Yu -Ying Yu, Ser - Sing Chang, Chien - Liang Lee, C. R. Chris Wang, Gold Nanorods: Electrochemical Synthesis and Optical Properties. The Journal of Physical Chemistry B 101,6661-6664(1997).
R. N. Viswanath, S. R. Polaki, R. Rajaraman, S. Abhaya, V. A. Chirayath, G. Amarendra, C. S. Sundar, On the scaling behaviour of hardness with ligament diameter of nanoporous Au: Constrained motion of dislocation along the ligaments. Appl. Phys. Lett. 104 233108 (1-4) (2014).
J. Erlebacher, M. J. Aziz, A. Karma, N. Dimitrov, K. Sieradzki, Evolution of nanoporosity in dealloying, Nature,410, 450-453 (2001).
S. Cherevko, A. A. Topalov, A. R. Zeradjanin, I. Katsounaros, K. J. J. Mayrhofer, Gold dissolution: towards understanding of noble metal corrosion, RSC Advances, 2013, 3: 16516 – 16527. http:// DOI: https://doi.org/10.1039/C3RA42684J
M. Balouiri, M. Sadiki, S. K. Ibnsouda, Methods for in vitro evaluating antimicrobial activity: A review, J. Pharmaceutical Analysis 6, 71 – 79 (2016).
C. Perez, M. Pauli, P. Bazerque, Acta Biologiae, An Antibiotic Assay by the Agar-Well Diffusion Method,” Acta Biologiae et Medecine Experimentalis15, 113-115 (1990).
Maher Obeidat, Mohamad Shatnawi, Mohammad Al-alawi, Enas Al-Zu`bi, Hanee Al-Dmoor, Maisa Al-Qudah, Jafar El-Qudah, Ismael Otri, Antimicrobial Activity of Crude Extracts of Plant Leaves, Research Journal of Microbiology7,59-67 (2012).
H-J. Jin, S. Parida, D. Kramer, J. Weissmüller, Sign-Inverted surface stress - charge response in nanoporous Au, Surface Science602,3588-3594 (2008).
B. E. Conway, Electrochemical oxide formation at noble metals as a surface chemical Process, Progress in Surface Science 49, 331-452 (1995).
C. Lakshmanan, R.N. Viswanath, S.R. Polaki, R. Rajaraman, S. Dash, A.K. Tyagi, Surface area of nanoporous gold: Effect on temperature, Electochemica Acta 182, 565 -572 (2015).
N. Huber, R. N. Viswanath, N. Mameka, J. Markmann, J. Weissmüller, Scaling laws of nanoporous metals under uniaxial compression, Acta Materialia 67 (252 - 265) (2014)
D. Jalas, R. Canchi, A. Yu. Petrov, S. Lang, L. Shao, J. Weissmüller, M. Eich, Effective medium model for the spectral properties of nanoporous gold in the visible, Applied Physics Letters105, 241906 (1-5) (2014).
S. Link, A.A. EI-Sayed, Simulation of the Optical Absorption Spectra of Gold Nanorods as a Function of Their Aspect Ratio and the Effect of the Medium Dielectric Constant – Additions and Corrections, J. Phys. Chm. B 109, 10531-10532. (2005).
K. Zheng, M. I. Setyawati, D. T. Leong, J. Xie, Antimicrobial Silver nanomaterials, Coordination Chemistry Reviews 357, 1 – 17 (2018).
Boyan Bonev, James Hooper, Judicaël Parisot, Principles of assessing bacterial susceptibility to antibiotics using the agar diffusion method, Journal of Antimicrobial Chemotherapy 61, 1295-1301 (2008).
K. E. Cooper, D. Woodman, The diffusion of antiseptics through agar gels, with special reference to the agar cup assay method of estimating the activity of penicillin J. Pathol. Bacteriol. 58, 75 - 84 (1946).
X. Li, S. M. Robinson, A. Gupta, K. Saha, Z. Jiang, D. F. Moyano, A, Sahar, M. A. Riley, V. M. Rotello, Functional Gold Nanoparticles as Potent Antimicrobial agents against Multi-drug, ACS NANO, 8 10682 – 10686 (2014).Resistant Bacteria.
N. Miasawa, M. Hakamada, M. Mabuchi, Effect of nanoporous Au on ATP synthase, MRS Communications (2020) doi:https://doi.org/10.1557/mrc.2020.8.
C. Lakshmanan, A. K. Behera, R.N. Viswanath, S. Amirthapandian,, R. Rajaraman, G. Amarendra, Microstructure Studies in nanoporousAu: Effects on electro-oxidation, Scripta Materialia 146, 68-72 (2018).
J. Thiel, L. Pakstis, S. Buzby. M. Raffi, C. Ni., D. J. Pochan, S. I. Shah, Antibacterial Properties of Silver-doped titania, Small3, 799 - 803 (2007).
J. A. Lemire, J. J. Harrison, R. J. Turner, Antimicobial activity of Metals: mechanisms, molecular targets and applications, Nature Reviews Microbiology 11, 371-384 (2013).
S. Sharmin, Md. M. Rahaman, C. Sarkar, O. Atolani, M. T. Islam, O. S. Adeyemi, Nanoparticlesas antimicrobial and antiviral agents: A literature-based perspectivestudy, Heliyon, 7 e06456 (1-9) (2021).
Y. N. Slavin, J. Asnis, U. O. Häfeli, H. Bach, Metal Nanoparticles: Understanding the mechanisms behind antibacterial activity, J. Nanotechnology 15 pp 1-20 (2017).
Acknowledgements
The authors acknowledge gratefully to Vinayaka Mission’s Research Foundation (VMRF), Chennai 603 104 for their constant research support. We thank IGCAR, Kalpakkam and UGC-DAE CSR Kalpakkam for providing electrochemical workstation and SEM facilities, respectively. We are grateful to Armats Bioteck Ltd., Chennai for antimicrobial testing. The financial support rendered by VMRF under a seed money project VMRF/SeedMoney-Phase2/ /2020-10/AVIT-Kanchi/11 is also gratefully acknowledged.
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NirmalaDevi, G., Viswanath, R.N., Suresh, G. et al. Synthesis and Microstructure Influenced Antimicrobial Properties of Dispersed Nanoporous Gold Rods. Trans Indian Inst Met 75, 2737–2747 (2022). https://doi.org/10.1007/s12666-022-02636-z
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DOI: https://doi.org/10.1007/s12666-022-02636-z