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Environmental Earth Sciences

, Volume 74, Issue 6, pp 4639–4648 | Cite as

Compositional and metabolic quotient analysis of heavy metal contaminated soil after electroremediation

  • Mahdi Bahemmat
  • Mohsen Farahbakhsh
  • Farzin Shabani
Original Article

Abstract

Heavy metal soil contamination provides a danger both to human and environmental ecosystem health and is still on the rise in many developing countries. Electroremediation provides an innovative method to remedy contamination of soils by heavy metals. Fundamental to the acceptance of any soil remedial technique is proof that positive benefits outweigh the negative impacts, in terms of soil health. The environmental effect of electroremediation of heavy metals contaminated soil by some biological indicators was evaluated. A soil contaminated with Zn, Pb, Ni, and Cd was used in a laboratory experiment. Treatment was imposed with a constant voltage gradient of 0.83 V/cm for 20 days. Results indicated that the physicochemical changes caused by the electroremidiation process on the soil microbial population (actinomycetes and gram-positive and negative bacteria), soil respiration and microbial biomass were significant (p < 0.01). At the commencement of the study, soil microbial activity was reduced, due to physicochemical changes in pH, amount of moisture and the changes in the levels of heavy metals in the soil. The greatest reduction on the microbial activity was close to the cathode where high levels of heavy metal concentrations and high pH were demonstrated. The metabolic quotient index [qCO2] was used to achieve a more refined evaluation, compared to analysis of soil respiration and microbial biomass alone. Accordingly, high amounts of qCO2 near the cathode show the unfavorable living conditions of such microorganisms in these sections.

Keywords

Electroremediation Heavy metals Metabolic quotient index Microbial activity Soil respiration 

References

  1. Acar YB, Alshawabkeh AN (1993) Principles of electrokinetic remediation. Environ Sci Technol 27:2638–2647CrossRefGoogle Scholar
  2. Aciego Pietri J, Brookes P (2009) Substrate inputs and pH as factors controlling microbial biomass, activity and community structure in an arable soil. Soil Biol Biochem 41:1396–1405CrossRefGoogle Scholar
  3. Alef K, Nannipieri P (1995) Methods in applied soil microbiology and biochemistry. Academic press, LondonGoogle Scholar
  4. Anderson T-H, Domsch K (1990) Application of eco-physiological quotients (qCO2 and qD) on microbial biomasses from soils of different cropping histories. Soil Biol Biochem 22:251–255CrossRefGoogle Scholar
  5. Anderson T-H, Domsch KH (2010) Soil microbial biomass: the eco-physiological approach. Soil Biol Biochem 42:2039–2043CrossRefGoogle Scholar
  6. Benintende SM, Benintende MC, Sterren MA, De Battista JJ (2008) Soil microbiological indicators of soil quality in four rice rotations systems. Ecol Indic 8:704–708CrossRefGoogle Scholar
  7. Brookes P, Heijnen CE, McGrath S, Vance E (1986) Soil microbial biomass estimates in soils contaminated with metals. Soil Biol Biochem 18:383–388CrossRefGoogle Scholar
  8. Cang L, Zhou D-M, Wang Q-Y, Wu D-Y (2009) Effects of electrokinetic treatment of a heavy metal contaminated soil on soil enzyme activities. J Hazard Mater 172:1602–1607CrossRefGoogle Scholar
  9. Cang L, Zhou D-M, Wang Q-Y, Fan G-P (2012) Impact of electrokinetic-assisted phytoremediation of heavy metal contaminated soil on its physicochemical properties, enzymatic and microbial activities. Electrochim Acta 86:41–48CrossRefGoogle Scholar
  10. Das S, Ram S, Sahu H, Rao D, Chakraborty A, Sudarshan M, Thatoi H (2013) A study on soil physico-chemical, microbial and metal content in Sukinda chromite mine of Odisha, India. Environ Earth Sci 69:2487–2497CrossRefGoogle Scholar
  11. El-Azeem SAA, Ahmad M, Usman AR, Kim K-R, Oh S-E, Lee SS, Ok YS (2013) Changes of biochemical properties and heavy metal bioavailability in soil treated with natural liming materials. Environ Earth Sci 70:3411–3420CrossRefGoogle Scholar
  12. Hillel D (1980) Fundamentals of soil physics. Academic Press Inc, Ltd, LondonGoogle Scholar
  13. Iskandar IK (2000) Environmental restoration of metals-contaminated soils. CRC Press, Boca RatonCrossRefGoogle Scholar
  14. Järup L (2003) Hazards of heavy metal contamination. Br Med Bull 68:167–182. doi: 10.1093/bmb/ldg032 CrossRefGoogle Scholar
  15. Jesußek A, Grandel S, Dahmke A (2013) Impacts of subsurface heat storage on aquifer hydrogeochemistry. Environ Earth Sci 69:1999–2012CrossRefGoogle Scholar
  16. Jia Y, Maurice C, Öhlander B (2014) Effect of the alkaline industrial residues fly ash, green liquor dregs, and lime mud on mine tailings oxidation when used as covering material. Environ Earth Sci 72:319–334CrossRefGoogle Scholar
  17. Khan M, Scullion J (2000) Effect of soil on microbial responses to metal contamination. Environ Pollut 110:115–125CrossRefGoogle Scholar
  18. Khan S, Cao Q, Hesham AE-L, Xia Y, He J-Z (2007) Soil enzymatic activities and microbial community structure with different application rates of Cd and Pb. J Environ Sci 19:834–840CrossRefGoogle Scholar
  19. Khan S, Cao Q, Zheng Y, Huang Y, Zhu Y (2008) Health risks of heavy metals in contaminated soils and food crops irrigated with wastewater in Beijing, China. Environ Pollut 152:686–692CrossRefGoogle Scholar
  20. Kieft TL, Rosacker LL (1991) Application of respiration-and adenylate-based soil microbiological assays to deep subsurface terrestrial sediments. Soil Biol Biochem 23:563–568CrossRefGoogle Scholar
  21. Kim S-H, Han H-Y, Lee Y-J, Kim CW, Yang J-W (2010) Effect of electrokinetic remediation on indigenous microbial activity and community within diesel contaminated soil. Sci Total Environ 408:3162–3168CrossRefGoogle Scholar
  22. Kwasniewska J, Nałęcz-Jawecki G, Skrzypczak A, Płaza G, Matejczyk M (2012) An assessment of the genotoxic effects of landfill leachates using bacterial and plant tests. Ecotoxicol Environ Saf 75:55–62CrossRefGoogle Scholar
  23. Lear G, Harbottle MJ, Van Der Gast C, Jackman S, Knowles C, Sills G, Thompson I (2004) The effect of electrokinetics on soil microbial communities. Soil Biol Biochem 36:1751–1760CrossRefGoogle Scholar
  24. Lear G, Harbottle MJ, Sills G, Knowles C, Semple KT, Thompson I (2007) Impact of electrokinetic remediation on microbial communities within PCP contaminated soil. Environ Pollut 146:139–146CrossRefGoogle Scholar
  25. Li T, Guo S, Wu B, Li F, Niu Z (2010) Effect of electric intensity on the microbial degradation of petroleum pollutants in soil. J Environ Sci 22:1381–1386CrossRefGoogle Scholar
  26. Li D, Tan X-Y, Wu X-D, Pan C, Xu P (2014) Effects of electrolyte characteristics on soil conductivity and current in electrokinetic remediation of lead-contaminated soil. Sep Purif Technol 135:14–21CrossRefGoogle Scholar
  27. Li T, Guo S, Wu B, Zhang L, Gao Y (2015) Effect of polarity reversal and electrical intensity on the oil removal from soil. J Chem Technol Biotechnol 90(3):441–448. doi: 10.1002/jctb.4312 CrossRefGoogle Scholar
  28. Liao M, Chen C-L, Zeng L-S, Huang C-Y (2007) Influence of lead acetate on soil microbial biomass and community structure in two different soils with the growth of Chinese cabbage (Brassica chinensis). Chemosphere 66:1197–1205CrossRefGoogle Scholar
  29. Moore J, Klose S, Tabatabai M (2000) Soil microbial biomass carbon and nitrogen as affected by cropping systems. Biol Fertil Soils 31:200–210CrossRefGoogle Scholar
  30. Muscolo A, Panuccio MR, Mallamaci C, Sidari M (2014) Biological indicators to assess short-term soil quality changes in forest ecosystems. Ecol Indic 45:416–423CrossRefGoogle Scholar
  31. Paramkusam BR, Srivastava RK, Mohan D (2015) Electrokinetic removal of mixed heavy metals from a contaminated low permeable soil by surfactant and chelants. Environ Earth Sci 73(3):1191–1204CrossRefGoogle Scholar
  32. Pazos M, Plaza A, Martín M, Lobo M (2012) The impact of electrokinetic treatment on a loamy-sand soil properties. Chem Eng J 183:231–237CrossRefGoogle Scholar
  33. Peng C, Almeira JO, Gu Q (2013) Effect of electrode configuration on pH distribution and heavy metal ions migration during soil electrokinetic remediation. Environ Earth Sci 69:257–265CrossRefGoogle Scholar
  34. Pula G et al (2009) Proteomics identifies thymidine phosphorylase as a key regulator of the angiogenic potential of colony-forming units and endothelial progenitor cell cultures. Circ Res 104:32–40CrossRefGoogle Scholar
  35. Reddy KR, Cameselle C (2009) Electrochemical remediation technologies for polluted soils, sediments and groundwater. Wiley, New YorkCrossRefGoogle Scholar
  36. Reddy KR, Chinthamreddy S (1999) Electrokinetic remediation of heavy metal-contaminated soils under reducing environments. Waste Manag 19:269–282CrossRefGoogle Scholar
  37. Richards BK, Steenhuis TS, Peverly JH, McBride MB (1998) Metal mobility at an old, heavily loaded sludge application site. Environ Pollut 99:365–377CrossRefGoogle Scholar
  38. Rozas F, Castellote M (2012) Electrokinetic remediation of dredged sediments polluted with heavy metals with different enhancing electrolytes. Electrochim Acta 86:102–109CrossRefGoogle Scholar
  39. Smith J, Paul E (1990) The significance of soil microbial biomass estimations. Soil Biochem 6:357–396Google Scholar
  40. Sparks D, Page A, Helmke P, Loeppert R (1996) Salinity: electrical conductivity and total dissolved solids, chap 14. In: Methods of soil analysis: chemical methods, part 3. SSSA Book Series no 5. Soil Science Society of America, pp 417–420Google Scholar
  41. Tiehm A, Lohner ST, Augenstein T (2009) Effects of direct electric current and electrode reactions on vinyl chloride degrading microorganisms. Electrochim Acta 54:3453–3459CrossRefGoogle Scholar
  42. Vance E, Brookes P, Jenkinson D (1987) An extraction method for measuring soil microbial biomass C. Soil Biol Biochem 19:703–707CrossRefGoogle Scholar
  43. Virkutyte J, Sillanpää M, Latostenmaa P (2002) Electrokinetic soil remediation—critical overview. Sci Total Environ 289:97–121CrossRefGoogle Scholar
  44. Wang Y, Shi J, Wang H, Lin Q, Chen X, Chen Y (2007) The influence of soil heavy metals pollution on soil microbial biomass, enzyme activity, and community composition near a copper smelter. Ecotoxicol Environ Saf 67:75–81CrossRefGoogle Scholar
  45. Wang Q-Y, Zhou D-M, Cang L, Li L-Z, Wang P (2009) Solid/solution Cu fractionations/speciation of a Cu contaminated soil after pilot-scale electrokinetic remediation and their relationships with soil microbial and enzyme activities. Environ Pollut 157:2203–2208CrossRefGoogle Scholar
  46. Wick LY et al (2010) Responses of soil microbial communities to weak electric fields. Sci Total Environ 408:4886–4893CrossRefGoogle Scholar
  47. Wuana RA, Okieimen FE (2011) Heavy metals in contaminated soils: a review of sources, chemistry, risks and best available strategies for remediation. ISRN Ecol 2011. doi: 10.5402/2011/402647 Google Scholar
  48. Yang L, Chen Z, Liu T, Jiang J, Li B, Chen S, Zhang J (2014) Soil respiratory and enzyme activities in leachate-contaminated soils with different application rate of cow manure compost: a laboratory study. Environ Earth Sci 71:225–231CrossRefGoogle Scholar
  49. Zhou D-M, Cang L, Alshawabkeh AN, Wang Y-J, Hao X-Z (2006) Pilot-scale electrokinetic treatment of a Cu contaminated red soil. Chemosphere 63:964–971CrossRefGoogle Scholar
  50. Zhou Y et al (2009) A combination method to study microbial communities and activities in zinc contaminated soil. J Hazard Mater 169:875–881CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Mahdi Bahemmat
    • 1
  • Mohsen Farahbakhsh
    • 1
  • Farzin Shabani
    • 2
  1. 1.Department of Soil Science, Faculty of Agricultural Engineering and TechnologyUniversity of TehranTehranIran
  2. 2.Ecosystem Management, School of Environmental and Rural ScienceUniversity of New EnglandArmidaleAustralia

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