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Indian Journal of Gastroenterology

, Volume 36, Issue 5, pp 390–399 | Cite as

Low-grade small intestinal bacterial overgrowth is common in patients with non-alcoholic steatohepatitis on quantitative jejunal aspirate culture

  • Uday C. GhoshalEmail author
  • Chalamalasetty Sreenivasa Baba
  • Ujjala Ghoshal
  • George Alexander
  • Asha Misra
  • Vivek A Saraswat
  • Gourdas Choudhuri
Original Article

Abstract

Background

Though pathogenesis of non-alcoholic steatohepatitis (NASH) is unclear, association with small intestinal bacterial overgrowth [SIBO] and fecal dysbiosis is suggested. We evaluated SIBO in NASH using quantitative jejunal aspirate culture (conventional criteria: ≥ 105 colony forming unit (CFU)/mL and newer cutoff ≥ 103 CFU/mL) and glucose hydrogen breath test.

Methods

Thirty-eight patients with NASH (age 37.5 years, range 20–54, 9, 24% female), diagnosed by ultrasonography, alanine aminotransferase >1.5 times normal and liver biopsy (in 27/38, 71%) and exclusion of other causes and 12 constipation-predominant irritable bowel syndrome as historical controls (age 39.5-y, 26–44; 3, 25% female) without fatty liver were studied.

Results

Jejunal aspirates, obtained in 35/38 patients, were sterile in 14/35 (40%) and bacteria isolated in 21 (60%) (all aerobic, in one anaerobe also; Gram positive 5, negative 13, both 3). In contrast, bacteria (two Gram negative) were isolated in 3/12 (25%) controls (odds ratio 4.5, 95% CI 1.0–19.5; p = 0.04); colony counts were higher in NASH than controls (median 380 CFU/mL, 0–200,000 vs. 0 CFU/mL, 0–1000; p = 0.02). Gram negative bacteria tended to be commoner in NASH than controls (16/35 vs. 2/12; p = 0.07). Seven out of 35 (20%) patients with NASH (≥ 105 CFU/mL in 5 and 2 other on glucose hydrogen breath test) and no control had SIBO (p = ns); low-grade SIBO (≥103 CFU/mL) was commoner in NASH than controls (14/35, 40%, vs. 1/12, 8.3%; p = 0.04). There was no correlation between bacterial colony count and bacterial type and anthropometric and biochemical parameters.

Conclusion

Low-grade bacterial overgrowth, particularly with Gram negative bacteria, was commoner in NASH than controls.

Keywords

Fatty liver Gut microbiota Hydrogen breath tests Metabolic disorder Pathogenesis 

Notes

Compliance with ethical standards

Conflict of interest

UCG, CSB, UG, GA, AM, VAS, and GC declare that they have no conflict of interest.

Ethics statement

The authors declare that the study was performed in a manner to conform with the Helsinki Declaration of 1975, as revised in 2000 and 2008, concerning Human and Animal Rights. The protocol was approved by the Institutional Ethics Committee and informed consent was obtained from the study subjects.

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Copyright information

© Indian Society of Gastroenterology 2017

Authors and Affiliations

  • Uday C. Ghoshal
    • 1
    Email author
  • Chalamalasetty Sreenivasa Baba
    • 1
  • Ujjala Ghoshal
    • 2
  • George Alexander
    • 1
  • Asha Misra
    • 1
  • Vivek A Saraswat
    • 1
  • Gourdas Choudhuri
    • 1
  1. 1.Department of GastroenterologySanjay Gandhi Postgraduate Institute of Medical SciencesLucknowIndia
  2. 2.Department of MicrobiologySanjay Gandhi Postgraduate Institute of Medical SciencesLucknowIndia

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