Skip to main content

Advertisement

SpringerLink
Log in

Fibrin Network and Platelets Densities in Platelet-Rich Fibrin (PRF) Membranes Produced from Plastic Tubes Without Additives: A New Approach to PRF Clinical Use

  • ORIGINAL ARTICLE
  • Published:
Journal of Maxillofacial and Oral Surgery Aims and scope Submit manuscript

Abstract

Background/Purpose

The present study aimed to investigate plastic tubes without additives as alternatives to glass and silica-coated plastic tubes, in the production of PRF membranes.

Materials and Methods

Nine blood samples were collected from eight volunteers (n = 8) separated into three groups, according to tube material: glass, silica-coated plastic, and plastic without additives. In each group, the samples were centrifuged using different relative centrifugation forces: L-PRF (700 g/12 min), A-PRF (200 g/14 min), and A-PRF + (200 g/8 min). The generated membranes were evaluated by histomorphometry, considering the fibrin network, platelet aggregates, and cellular morphology, by light microscopy. The ultrastructural cellular morphology integrity was evaluated by transmission electron microscopy.

Results

The L-PRF (p < 0.019) and A-PRF (p < 0.001) membranes showed a significantly lower fibrin network density in plastic tubes without additives compared to glass and silica-coated plastic tubes. Plastic tubes without additives revealed a significantly higher platelet percentage, regardless of the protocol (p < 0.005). In all groups, TEM analysis showed preserved normal morphological ultrastructure, maintaining the integrity of cellular components.

Conclusion

Plastic tubes without additives offer a viable alternative for producing PRF membranes. They exhibited a higher platelet density and demonstrated fibrin network and cellular morphology similar to those of glass and silica-coated plastic tubes, irrespective of the centrifugation protocol.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Choukroun J, Diss A, Simonpieri A et al (2006) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: clinical effects on tissue healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101(3):e56–e60. https://doi.org/10.1016/j.tripleo.2005.07.011

    Article  PubMed  Google Scholar 

  2. Dohan DM, Choukroun J, Diss A et al (2006) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 101(3):e45–e50. https://doi.org/10.1016/j.tripleo.2005.07.009

    Article  PubMed  Google Scholar 

  3. Dohan DM, Choukroun J, Diss A et al (2006) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part III: leucocyte activation: a new feature for platelet concentrates? Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 101(3):e51–e55. https://doi.org/10.1016/j.tripleo.2005.07.010

    Article  PubMed  Google Scholar 

  4. Choukroun J, Adda F, Schoeffler C, Vervelle A (2000) Une opportunité en paro-implantologie: le PRF. Implantodontie 42:55–62

    Google Scholar 

  5. Dohan DM, Choukroun J, Diss A et al (2006) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 101(3):e37–e44. https://doi.org/10.1016/j.tripleo.2005.07.008

    Article  PubMed  Google Scholar 

  6. Choukroun J, Ghanaati S (2018) Reduction of relative centrifugation force within injectable platelet-rich-fibrin (PRF) concentrates advances patients’ own inflammatory cells, platelets and growth factors: the first introduction to the low speed centrifugation concept. Eur J Trauma Emerg Surg 44(1):87–95. https://doi.org/10.1007/s00068-017-0767-9

    Article  CAS  PubMed  Google Scholar 

  7. Fujioka-Kobayashi M, Miron RJ, Hernandez M, Kandalam U, Zhang Y, Choukroun J (2017) Optimized platelet-rich fibrin with the low-speed concept: growth factor release, biocompatibility, and cellular response. J Periodontol 88(1):112–121. https://doi.org/10.1902/jop.2016.160443

    Article  CAS  PubMed  Google Scholar 

  8. Miron RJ, Fujioka-Kobayashi M, Hernandez M et al (2017) Injectable platelet rich fibrin (i-PRF): opportunities in regenerative dentistry? Clin Oral Investig 21(8):2619–2627. https://doi.org/10.1007/s00784-017-2063-9

    Article  PubMed  Google Scholar 

  9. Miron RJ, Xu H, Chai J et al (2020) Comparison of platelet-rich fibrin (PRF) produced using 3 commercially available centrifuges at both high (~ 700 g) and low (~ 200 g) relative centrifugation forces. Clin Oral Investig 24(3):1171–1182. https://doi.org/10.1007/s00784-019-02981-2

    Article  PubMed  Google Scholar 

  10. Kratz A, Stanganelli N, Van Cott EM (2006) A comparison of glass and plastic blood collection tubes for routine and specialized coagulation assays: a comprehensive study. Arch Pathol Lab Med 130(1):39–44. https://doi.org/10.5858/2006-130-39-ACOGAP

    Article  PubMed  Google Scholar 

  11. Tsujino T, Masuki H, Nakamura M et al (2019) Striking differences in platelet distribution between advanced-platelet-rich fibrin and concentrated growth factors: effects of silica-containing plastic tubes. J Funct Biomater 10(3):43. https://doi.org/10.3390/jfb10030043

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Tsujino T, Takahashi A, Yamaguchi S et al (2019) Evidence for contamination of silica microparticles in advanced platelet-rich fibrin matrices prepared using silica-coated plastic tubes. Biomedicines 7(2):45. https://doi.org/10.3390/biomedicines7020045

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Yamaguchi S, Aizawa H, Sato A et al (2020) Concentrated growth factor matrices prepared using silica-coated plastic tubes are distinguishable from those prepared using glass tubes in platelet distribution: application of a novel near-infrared imaging-based quantitative technique. Front Bioeng Biotechnol 8:600. https://doi.org/10.3389/fbioe.2020.00600

    Article  PubMed  PubMed Central  Google Scholar 

  14. Miron RJ, Kawase T, Dham A, Zhang Y, Fujioka-Kobayashi M, Sculean A (2021) A technical note on contamination from PRF tubes containing silica and silicone. BMC Oral Health 21(1):135. https://doi.org/10.1186/s12903-021-01497-0

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Chen L, Liu J, Zhang Y et al (2018) The toxicity of silica nanoparticles to the immune system. Nanomedicine (Lond) 13(15):1939–1962. https://doi.org/10.2217/nnm-2018-0076

    Article  PubMed  Google Scholar 

  16. Sharma N, Jha S (2020) Amorphous nanosilica induced toxicity, inflammation and innate immune responses: a critical review. Toxicology 441:152519. https://doi.org/10.1016/j.tox.2020.152519

    Article  CAS  PubMed  Google Scholar 

  17. Murugadoss S, Lison D, Godderis L et al (2017) Toxicology of silica nanoparticles: an update. Arch Toxicol 91(9):2967–3010. https://doi.org/10.1007/s00204-017-1993-y

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Yazdimamaghani M, Moos PJ, Dobrovolskaia MA, Ghandehari H (2019) Genotoxicity of amorphous silica nanoparticles: status and prospects. Nanomedicine 16:106–125. https://doi.org/10.1016/j.nano.2018.11.013

    Article  CAS  PubMed  Google Scholar 

  19. Masuki H, Isobe K, Kawabata H et al (2020) Acute cytotoxic effects of silica microparticles used for coating of plastic blood-collection tubes on human periosteal cells. Odontology 108(4):545–552. https://doi.org/10.1007/s10266-020-00486-z

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Margolis J (1957) Initiation of blood coagulation by glass and related surfaces. J Physiol 137(1):95–109. https://doi.org/10.1113/jphysiol.1957.sp005799

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Golas A, Parhi P, Dimachkie ZO, Siedlecki CA, Vogler EA (2010) Surface-energy dependent contact activation of blood factor XII. Biomaterials 31(6):1068–1079. https://doi.org/10.1016/j.biomaterials.2009.10.039

    Article  CAS  PubMed  Google Scholar 

  22. Saboia-Dantas CJ, Limirio PHJO, Costa MDMA, Linhares CRB, Silva MAFS, Oliveira HAABO et al (2022) Platelet-rich fibrin progressive protocol: third generation of blood concentrates. J Oral Maxillofac Surg. https://doi.org/10.1016/j.joms.2022.09.002

    Article  PubMed  Google Scholar 

  23. Weisel JW, Litvinov RI (2013) Mechanisms of fibrin polymerization and clinical implications. Blood 121(10):1712–1719. https://doi.org/10.1182/blood-2012-09-306639

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Ryan EA, Mockros LF, Weisel JW, Lorand L (1999) Structural origins of fibrin clot rheology. Biophys J 77(5):2813–2826. https://doi.org/10.1016/S0006-3495(99)77113-4

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Kubesch A, Barbeck M, Al-Maawi S et al (2019) A low-speed centrifugation concept leads to cell accumulation and vascularization of solid platelet-rich fibrin: an experimental study in vivo. Platelets 30(3):329–340. https://doi.org/10.1080/09537104.2018.1445835

    Article  CAS  PubMed  Google Scholar 

  26. Golebiewska EM, Poole AW (2015) Platelet secretion: From haemostasis to wound healing and beyond. Blood Rev 29(3):153–162. https://doi.org/10.1016/j.blre.2014.10.003

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Nurden AT (2011) Platelets, inflammation and tissue regeneration. Thromb Haemost 105(Suppl 1):S13–S33. https://doi.org/10.1160/THS10-11-0720

    Article  CAS  PubMed  Google Scholar 

  28. Gawaz M, Vogel S (2013) Platelets in tissue repair: control of apoptosis and interactions with regenerative cells. Blood 122(15):2550–2554. https://doi.org/10.1182/blood-2013-05-468694

    Article  CAS  PubMed  Google Scholar 

  29. Tunalı M, Özdemir H, Küçükodacı Z et al (2014) A novel platelet concentrate: titanium-prepared platelet-rich fibrin. Biomed Res Int 2014:209548. https://doi.org/10.1155/2014/209548

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Bhattacharya HS, Gummaluri SS, Astekar M, Gummaluri RK (2020) Novel method of determining the periodontal regenerative capacity of T-PRF and L-PRF: an immunohistochemical study. Dent Med Probl 57(2):137–144. https://doi.org/10.17219/dmp/117721

    Article  PubMed  Google Scholar 

  31. Tsukioka T, Hiratsuka T, Nakamura M et al (2019) An on-site preparable, novel bone-grafting complex consisting of human platelet-rich fibrin and porous particles made of a recombinant collagen-like protein. J Biomed Mater Res B Appl Biomater 107(5):1420–1430. https://doi.org/10.1002/jbm.b.34234

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The authors would like to thank Dental Research Center Biomechanics, Biomaterials and Cell Biology (Federal University of Uberlandia) for providing a large part of the structure used in the research.

Funding

This work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES/Brazil)-Finance Code 001-nd Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG/Brazil) for the granting of scholarship and resource for the purchase of consumer material.

Author information

Authors and Affiliations

  1. Dental School, Federal University of Uberlandia, Uberlândia, Minas Gerais, Brazil

    Maria Adelia Faleiro Santana Silva, Camila Rodrigues Borges Linhares, Pedro Henrique Justino Oliveira Limirio & Marcelo Dias Moreira de Assis Costa

  2. Laboratory of Tissue Repair Research, Brain Storm Academy, Federal University of Uberlandia, Uberlândia, Minas Gerais, Brazil

    Carlos José Saboia-Dantas

  3. Department of Oral Surgery and Implantology, Brazilian Dental Association, Uberlândia, Minas Gerais, Brazil

    Hany Angelis Abadia Borges de Oliveira

  4. Department of Cell Biology, Histology and Embryology, Biomedical Science Institute, Federal University of Uberlandia, Avenida Pará 1720, Campus Umuarama, Bloco 2B, Bairro Umuarama, Uberlândia, Minas Gerais, 38.400-902, Brazil

    Rosiane Nascimento Alves & Paula Dechichi

  5. Biological Sciences Course, State University of Minas Gerais, Ituiutaba, Minas Gerais, Brazil

    Rosiane Nascimento Alves

Authors
  1. Maria Adelia Faleiro Santana Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Camila Rodrigues Borges Linhares
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Carlos José Saboia-Dantas
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Pedro Henrique Justino Oliveira Limirio
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Marcelo Dias Moreira de Assis Costa
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hany Angelis Abadia Borges de Oliveira
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Rosiane Nascimento Alves
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Paula Dechichi
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by MAFSS, CRBL, PHJOL, MDMAC, HAABO, and PD. The first draft of the manuscript was written by MAFSS, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Paula Dechichi.

Ethics declarations

Conflict of interest

The authors have no relevant financial or non-financial interests to disclose.

Ethical Approval

This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of Federal University of Uberlandia (No. 13857519.0.0000.5152).

Consent to Participate

Informed consent was obtained from all individual participants included in the study.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Silva, M.A.F.S., Linhares, C.R.B., Saboia-Dantas, C.J. et al. Fibrin Network and Platelets Densities in Platelet-Rich Fibrin (PRF) Membranes Produced from Plastic Tubes Without Additives: A New Approach to PRF Clinical Use. J. Maxillofac. Oral Surg. (2024). https://doi.org/10.1007/s12663-023-02103-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s12663-023-02103-2

Keywords

Search

Navigation