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Salivary Cortisol as a Stress Monitor During Third Molar Surgery

Journal of Maxillofacial and Oral Surgery volume 21, pages 1112–1118 (2022)Cite this article

Abstract

Background

Procedures in oral surgery, especially surgical extraction of the impacted mandibular third molar is often considered anxiety-producing and stressful. This study evaluated the effect of oral sedation (5 mg diazepam) on the physiological stress level in subjects that underwent surgical extraction of the mandibular third molar by measuring the change in salivary cortisol concentration.

Subjects and methods

204 salivary samples were collected from 102 subjects between 9.00 am and 12.00 pm to standardise the diurnal variations of cortisol secretion. Saliva samples were collected 45 min before and 15 min after surgical extraction from each subject in either group. The samples were stored in the freezer (− 20 °C) until analysis was done in the laboratory using salivary cortisol ELISA kits (DiaMetra S.r.l., Eagle Biosciences, Italy), and the cortisol concentration was measured with a microplate reader.

Results

There was a statistically significant change (p = <0.001 between the pre-surgical extraction salivary cortisol concentration of all subjects with a median of 7 ng/ml and post-surgical extraction salivary cortisol concentration of both the study and the control groups with a median of 17 ng/ml and 15 ng/ml, respectively. Only 11.8% of subjects in the study group had a reduction in post-surgical salivary cortisol concentration, while in the control group, 3.9% of subjects had a reduction in post-surgical salivary cortisol concentration. There was no statistically significant difference between the two groups (p = 0.135).

Conclusions

Hence, oral sedation has no significant impact on physiological stress during the surgical extraction of the mandibular third molar. However, salivary cortisol concentration can adequately reflect the stress induced by surgical extraction in subjects and its usefulness as a biomarker in stress research. Furthermore, the type of disimpaction of mandibular third molar affects salivary cortisol concentration, with distoangular disimpaction having the highest cortisol concentration and more stressful to subjects when compared to other types of disimpaction.

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, Lagos State University Teaching Hospital, 1-5, Oba Akinjobi Road, Ikeja, Lagos, Nigeria

    Taofiq Opaleye, Eyituoyo Okoturo, Oluwafemi Adewale Adesina, Yussuf Salami & John Chukwudumebi Wemambu

  2. Lagos State University College of Medicine (LASUCOM), Ikeja, Lagos State, Nigeria

    Eyituoyo Okoturo & Oluwafemi Adewale Adesina

  3. Department of Preventive Dentistry, Faculty of Dentistry, Lagos State University College of Medicine, Ikeja, Lagos State, Nigeria

    Afolabi Oyapero

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  1. Taofiq Opaleye
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  2. Eyituoyo Okoturo
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  3. Oluwafemi Adewale Adesina
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  4. Afolabi Oyapero
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  5. Yussuf Salami
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  6. John Chukwudumebi Wemambu
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Correspondence to Eyituoyo Okoturo.

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Ethical Aapproval

All procedures performed in this study involving human participants were in accordance with the ethical standards of the Health Research and Ethics Committee of the Lagos State University Teaching Hospital.

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Informed consent was obtained from all individual participants included in the study.

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Opaleye, T., Okoturo, E., Adesina, O.A. et al. Salivary Cortisol as a Stress Monitor During Third Molar Surgery. J. Maxillofac. Oral Surg. 21, 1112–1118 (2022). https://doi.org/10.1007/s12663-020-01480-2

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  • DOI: https://doi.org/10.1007/s12663-020-01480-2

Keywords

  • Stress
  • Saliva
  • Cortisol
  • ELISA
  • Sedatives