Journal of Maxillofacial and Oral Surgery

, Volume 17, Issue 2, pp 254–259 | Cite as

Buccal Mucosal Epithelial Cells Downregulate CTGF Expression in Buccal Submucosal Fibrosis Fibroblasts

  • Sanjay Gottipamula
  • Sudarson Sundarrajan
  • Aditya Moorthy
  • Sriram Padmanabhan
  • K. N SridharEmail author
Original Article



Oral submucosal fibrosis (OSMF) is a chronic debilitating fibrotic disease of the oral cavity and is a serious health hazard in south Asia and, increasingly, the rest of the world. The molecular basis behind various treatment modalities to treat OSMF still remains unclear. In this study, we have investigated the in vitro ability of the buccal mucosal cells to reduce the proliferation of the fibroblasts of the fibrotic area in co-culture of cells and also at the molecular levels to reduce the level of connective tissue growth factor (CTGF) in the OSMF fibroblasts (SMF-F).

Materials and Methods

The study compares isolation, morphological and proliferation kinetics of SMF-F and BMF cells with and without co-culturing with BMEs. In addition, we have compared the mRNA expression levels of CTGF in SMF-F co-cultured BME and non-co-cultured SMF-F cells using validated real-time quantitative PCR (RT-qPCR) method.


The basic morphological characteristics of SMF-F were similar to BMF, but the former cells had higher proliferation rate in early passages compared to late passage state. We also observed that the CTGF expression levels in SMF-F under co-culture conditions of BME were consistently and significantly downregulated in all four different SMF-F-derived cells from four different patients.


Rapid proliferation and collagen synthesis in SMF-F as against BMF cells are the factors that confirm the innate nature of fibrosis fibroblasts (SMF-F). Further, the CTGF expression level in SMF-F was significantly suppressed by BME in co-culture conditions against controls (BMF). Considered together, this suggests that the cell therapeutic candidate of BME could be used in treating OSMF.


Buccal epithelial cells Connective tissue growth factor Fibroblasts Oral submucosal fibrosis 



SRTE is fully supported by Sri Sringeri Sharada Peetam.

Compliance with Ethical Standards

Conflict of interest

The author discloses no conflict of interest.


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Copyright information

© The Association of Oral and Maxillofacial Surgeons of India 2017

Authors and Affiliations

  • Sanjay Gottipamula
    • 1
  • Sudarson Sundarrajan
    • 2
  • Aditya Moorthy
    • 3
  • Sriram Padmanabhan
    • 2
  • K. N Sridhar
    • 1
    • 2
    Email author
  1. 1.Sri Research for Tissue Engineering Pvt. Ltd, Shankara Research CentreRangadore Memorial HospitalBangaloreIndia
  2. 2.Cancyte Research Pvt. LtdRangadore Memorial HospitalBangaloreIndia
  3. 3.Oral and Maxillofacial DepartmentRangadore Memorial HospitalBangaloreIndia

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