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Degradation of Rice Straw in the Presence of ZnO Nanoparticles and Cellulase Production with the Help of Streptomycetes Species

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Abstract

This study aims to determine how ZnO nanoparticles affect rice straw during solid-state fermentation and cellulase production. The zinc oxide (ZnO) nanoparticles of concentration 1 mg/gm was supplemented in a solid-state fermentation medium with the isolated actinobacterial species Streptomycetes calvus for cellulase production. It was also noticed that the addition of the nanoparticles catalyzed the biodegradation of rice straw complex structure and facilitates cellulase production. So, higher cellulase activities were observed in the enzymes produced in the presence of the ZnO, i.e., 0.38 IU/ml, followed by 0.27 IU/ml in control (without nanoparticles). Further, XRD, SEM and FTIR clearly showed the degradation of lignocellulose and morphological changes in rice straw structure during fermentation. Additionally, higher FPases activities and saccharification percentage indicated that nanoparticle-mediated cellulases would be a better option for further applications.

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Acknowledgements

The authors are thankful to the Central Research Facility (CRF), IIT Delhi, for providing sophisticated instrumentation facility support for analysis. The authors also thank the Head of CRDT for providing basic facilities. The authors are grateful to MHRD and IIT Delhi for fellowship support.

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The authors have not disclosed any funding from external sources.

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Authors and Affiliations

  1. Agri-Nanotechnology Laboratory, Centre for Rural Development and Technology (CRDT), Indian Institute of Technology Delhi, New Delhi, 110016, India

    Zoya Javed, Gyan Datta Tripathi, Meghana Gattupalli & Kavya Dashora

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  1. Zoya Javed
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  2. Gyan Datta Tripathi
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  3. Meghana Gattupalli
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  4. Kavya Dashora
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Contributions

All the authors contributed to the conceptualization of the work. ZJ and GDT contributed to in vitro studies, data collection, result analysis and writing of the manuscript. MG Review and editing KD Supervised, proof reading, review and editing.

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Correspondence to Kavya Dashora.

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Javed, Z., Tripathi, G.D., Gattupalli, M. et al. Degradation of Rice Straw in the Presence of ZnO Nanoparticles and Cellulase Production with the Help of Streptomycetes Species. Waste Biomass Valor 15, 3045–3053 (2024). https://doi.org/10.1007/s12649-023-02374-3

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