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Comparison of Antioxidant, Antiradical and Antibacterial Activities of Mistletoe (Viscum album L.) Fruits and Leaves Growing on Different Host Tree Genus

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Abstract

Mistletoe is a hemi-parasitic shrub and grows on different host trees and used as a remedy for some disease therapies. In this study, mistletoe fruits and leaves growing on different host tree genera were compared in terms bioactivity and antibacterial efficiency. For this purpose, mistletoe samples were collected from seven different tree genera (black locust, wild pear, pine, hawthorn, willow, apricot and almond) and removed their fruits and leaves. At the beginning, the most suitable solvent for the extraction was determined using optimization procedure of simplex lattice mixture design. The most effective solvent showing the highest total phenolic content (TPC) and antiradical activity (ARA) was determined as ethanol:water (92.5:7.5) for the fruit and ethanol:water (30:70) for the leaf extraction. TPC values of the leaf and fruit samples ranged between 7 and 21.2 and 3.2–6.9 mg GAE/g, respectively. The results showed significant differences based on the tree genus and the highest antioxidant and antiradical activities were monitored for the black locust tree while the lowest values were in willow tree. For antibacterial activity, the highest inhibition zone was obtained from wild pear leaf extract for Salmonella Typhimurium. Mistletoe leave extracts showed a remarkable superiority bioactive performance than those of the mistletoe fruits for all tree genus.

Graphical Abstract

Experimental design showing the workflow for the bioactive performance of mistletoe fruit and leaf collected from different tree genus.

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Acknowledgements

We would like to thank to Scientific Research Project Unit of Erciyes University for financial support.

Funding

This study was supported as financially by Scientific Research Project Unit of Erciyes University (Project Code: FYL-2020-10193).

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Authors and Affiliations

  1. Department of Field Crops, Faculty of Agriculture, Erciyes University, Kayseri, Turkey

    Beyza Çiftci & Mahmut Kaplan

  2. Department of Agricultural Biotechnology, Faculty of Agriculture, Erciyes University, Kayseri, Turkey

    Kevser Karaman

Authors
  1. Beyza Çiftci
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  2. Kevser Karaman
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  3. Mahmut Kaplan
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Contributions

BC: Experimental analysis, Data analysis, KK: Co-supervision, Experimental design and analysis, Optimization, Writing,Review and Editing. MK: Supervision, Sample collection, Experimental design and analysis Writing, Editing.

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Correspondence to Beyza Çiftci.

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Çiftci, B., Karaman, K. & Kaplan, M. Comparison of Antioxidant, Antiradical and Antibacterial Activities of Mistletoe (Viscum album L.) Fruits and Leaves Growing on Different Host Tree Genus. Waste Biomass Valor 15, 2819–2832 (2024). https://doi.org/10.1007/s12649-023-02307-0

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