Abstract
The cultivation of oyster mushrooms is becoming increasingly popular around the world due to their ability to transfer lignocellulosic waste into balanced food. Therefore, in this current study, we focus on the domestication of Pleurotus spp., Pleurotus eryngii var. ferulae, and Pleurotus eryngii var. elaeoselini on different media, cereal grains, and various lignocellulosic substrates. Results reveal that Malt Extract Agar is suitable as a medium for the domestication of the two strains in comparison with Potato Dextrose Agar. This result is proven by its high growth rate (64.33%) with Pleurotus eryngii var. elaeoselini and (52.42%) with Pleurotus eryngii var. ferulae. Lolium perenne grains have the shortest duration to generate a spawn for Pleurotus eryngii var. ferulae. Ten substrate formulas were evaluated in this study as follows T1 (100% wheat straw), T2 (100% cardboard), T3 (100% spent coffee grounds), T4 (100% olive pomace), T5 (50% wheat straw + 50% Cardboard); T6 (75% wheat straw + 25% Cardboard 5% wheat bran); T7 (50% wheat straw + 50% Coffee ground), T8 (75% wheat straw + 25% Coffee grounds + 5% wheat bran), T9 (50% Wheat straw + 50% Olive pomace), T10 (75% wheat straw + 25% Olive pomace + 5% wheat bran). For Pleurotus eryngii var. elaeoselini cultivation, T5 (50% wheat straw + 50% Cardboard) has the significantly highest number of primordia with 30 primordia after 27 days of incubation. Regarding Pleurotus eryngii var. ferulae, the primordia number was significantly high with T8 (75% wheat straw + 25% spent coffee grounds + 5% wheat bran), with about 16 primordia after 44 days.
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Acknowledgements
The authors would like to acknowledge Dr. Pierre Roux, a mycologist in the University of Pharmacia in Lyon, France.
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All authors jointly carried out the study; CJ Conceptualization, Methodology, Formal analysis, Investigation, interpretation, Data curation, Writing; AK Methodology, Validation, Review and editing; SAS: Supervision.
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Jaffali, C., Khadhri, A. & Aschi-Smiti, S. Domestication of Pleurotus eryngii Mycelium and Primordia Formation on Various Agricultural Residues. Waste Biomass Valor 15, 1113–1122 (2024). https://doi.org/10.1007/s12649-023-02239-9
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DOI: https://doi.org/10.1007/s12649-023-02239-9