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Production, Purification, Characterization, and Application of Halotolerant and Thermostable Endoglucanase Isolated from Pycnoporus sanguineus

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Abstract

Agribusiness is the main branch of the Brazilian economy. Every year, millions of tons of agro-industrial waste are generated and improperly disposed on soil and water, generating social and environmental impacts. However, these wastes can be used as substrate for fungal growth and to produce enzymes of biotechnological interest (hydrolases, proteases, lipases). In this study, we present the production of an endoglucanase from Pycnoporus sanguineus (the first enzyme isolated from this organism) by solid-state fermentation of agro-industrial wastes. Endoglucanase was produced after 72 fermentation hours, and was isolated via ethanol fractionation and ion exchange chromatography. The isolated enzyme had an optimum temperature of 50 °C, thermostability between 30 and 60 °C, optimum pH of 5.0, stability at pH values from 5.0 to 8.0, KM of 3.18 ± 0.005 mg/mL, and kcat of 4.53 s−1. It also displayed halophilic properties. Additionally, endoglucanase was able to saccharify three kinds of agro-industrial waste in 24 h without any chemical or physical pretreatment. Rice husk, wheat bran, and sugarcane bagasse produced 273, 192, and 155 mg/mL of reducing sugars after enzymatic hydrolysis, respectively. Thus, enzyme shows to be a promising option for obtaining fermentable sugars from agro-industrial waste for the production of second-generation ethanol.

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  1. Institute of Chemistry and Biotechnology, Federal University of Alagoas, A. C. Simões Campus, (UFAL), Maceió, Alagoas, 57072-900, Brazil

    Dávida Maria Ribeiro Cardoso dos Santos, Fabiana Albuquerque, Tatielle Pereira Silva, Alexsandra Nascimento Ferreira, Sônia Salgueiro Machado & Hugo Juarez Vieira Pereira

  2. 2Laboratório de Associações Micorrizicas - LAMIC, Departamento de Microbiologia, Universidade Federal de Viçosa (UFV), Avenida Ph Rolfs S/N, Viçosa, Minas Gerais-Mg, 36570-000, Brazil

    José Maria Rodrigues da Luze

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dos Santos, D.M.R.C., Albuquerque, F., Silva, T.P. et al. Production, Purification, Characterization, and Application of Halotolerant and Thermostable Endoglucanase Isolated from Pycnoporus sanguineus. Waste Biomass Valor 14, 3211–3222 (2023). https://doi.org/10.1007/s12649-023-02175-8

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