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Biodegradation and Detoxification of Malachite Green Dye by Extracellular Laccase Expressed from Fusarium oxysporum

Waste and Biomass Valorization volume 13, pages 2511–2518 (2022)Cite this article

Abstract

The present study evaluated the potential of biodegrading the toxic synthetic dye malachite green (MG) by an enzymatic approach. Extracellular laccase derived a fungal strain Fusarium oxysporum HUIB02 was used in the direct biodegradation of MG. Time-course study showed that the crude laccase successfully removed ≥ 80% MG after 20 h of treatment. The presence of Cu2+ ions enhanced MG degradation, while cation Fe2+ and anions including Cl− and I− reduced the degradation efficiency. The optimal temperature was 40 °C. Supplementation of mediators including syringaldehyde (SA), 1-hydroxybenzotriazole (HBT), and vanillin (VA) improved the MG degradation up to 99%. Under the optimal reaction conditions, the MG degradation efficiency of laccase reached ≥ 90% when MG concentration was ≤ 100 mg/L. At a higher concentration (1000 mg/L of MG), the enzyme still removed 88.1% the present dye. The detoxification evaluation confirmed the nullification of MG toxicity on microorganism after the enzymatic treatment. Hence, this enzymatic degradation approach is promising for the treatment of effluents containing MG.

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Funding

This research was funded by Vietnam National Foundation for Science and Technology Development (NAFOSTED) under Grant Number 106.04-2018.51. The authors also acknowledge the partial support of Hue University under the Core Research Program, Grant No. NCM.DHH.2020.11.

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Authors and Affiliations

  1. Institute of Biotechnology, Hue University, Hue, 49000, Vietnam

    Le Thi Kim Thoa, Trinh Thi Phuong Thao, Nguyen Bao Hung, Hoang Tan Quang, Tran Thuy Lan & Nguyen Duc Huy

  2. Faculty of Applied Sciences, UCSI University, UCSI Heights, 56000, Cheras, Kuala Lumpur, Malaysia

    Kuan Shiong Khoo

  3. University of Science and Education, The University of Danang, Danang, 50000, Vietnam

    Vu Duc Hoang

  4. College of Environmental and Bioresource Sciences, Jeonbuk National University, Iksan, 570–752, Jeonbuk, South Korea

    Seung-Moon Park

  5. Chemical Engineering Discipline and Advanced Engineering Platform, School of Engineering, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500, Subang Jaya, Selangor Darul Ehsan, Malaysia

    Chien Wei Ooi

  6. Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, 43500, Semenyih, Selangor Darul Ehsan, Malaysia

    Pau Loke Show

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  1. Le Thi Kim Thoa
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  2. Trinh Thi Phuong Thao
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  10. Pau Loke Show
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  11. Nguyen Duc Huy
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by LTKT and NDH. The first draft of the manuscript was written by LTKT. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Pau Loke Show or Nguyen Duc Huy.

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Thoa, L.T.K., Thao, T.T.P., Hung, N.B. et al. Biodegradation and Detoxification of Malachite Green Dye by Extracellular Laccase Expressed from Fusarium oxysporum. Waste Biomass Valor 13, 2511–2518 (2022). https://doi.org/10.1007/s12649-022-01692-2

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  • DOI: https://doi.org/10.1007/s12649-022-01692-2

Keywords

  • Bioremediation
  • Detoxification
  • Fusarium oxysporum
  • Laccase
  • Malachite green