Abstract
In this study, the combined strategy of using high solid load, on-site enzyme cocktails and surfactant was evaluated in saccharifications of hydrothermally pretreated sugarcane bagasse (HP-SB) and ethanol production. The hydrolyses were carried in fed-batch mode with a solid load of 10–40% (w/v) at time intervals of 12 h, using two homemade enzyme extracts (ES1 from Aspergillus niger monoculture and ES2 from A. niger, Trametes versicolor and Pleurotus ostreatus consortium), 10 FPU/gds of cellulase loading at 50 °C for 72 h. After optimization of solid loading, new saccharifications were performed with the addition of 5% (w/v) surfactant (Triton X-100). The HP of SB led to a significant reduction of 69.26% in hemicelluloses content, but also preserved the cellulose fraction in HP-SB. The increase of HP-SB load in hydrolysis from 10 to 35% significantly improved the release of total reducing sugars (TRS), with an increase of 188.54% in ES1 and 177.46% in ES2. The use of Triton X-100 in saccharifications of HP-SB (30% w/v) also positively contributed to TRS production, with an increase in TRS of 6.22% in ES1S and 24% in ES2S. The fermentation of the hydrolysate after surfactant-assisted hydrolysis of HP-SB (30% w/v) led to an ethanol yield of 81.70% for F1S and 88.03% for F2S. Results demonstrated that the integrated use of high solid load, low-cost on-site enzyme cocktail and surfactant (Triton X-100) can be a promising approach to improve the efficiency of bioconversion of lignocellulosic biomass to fermentable sugars.
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10 February 2022
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Acknowledgements
The authors are grateful to the Federal University of Uberlandia (UFU) and the Federal University of Ouro Preto (UFOP) for the technical support. This study was financed by Minas Gerais State Research Funding Agency (FAPEMIG grant numbers APQ-01360-14 and CEX-PPM-00645-17). The authors would also like to thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES), the Brazilian National Council for Scientific and Technological Development (CNPq) and the Brazilian Study and Project Funding Agency (FINEP) for funding this research. Patrísia de Oliveira Rodrigues is grateful to FAPEMIG for her Doctorate fellowship.
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POR: conceptualization, methodology, validation, formal analysis, investigation, writing—original draft and editing. FSM, VLC and LDS: methodology, validation, formal analysis. LVAG: methodology, formal analysis, resources, writing review. DP: methodology, formal analysis, resources, writing review, funding acquisition. MAB: conceptualization, methodology, formal analysis, resources, writing review & editing, supervision, project administration, funding acquisition.
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de Oliveira Rodrigues, P., Moreira, F.S., Cardoso, V.L. et al. Combination of High Solid Load, On-site Enzyme Cocktails and Surfactant in the hydrolysis of Hydrothermally Pretreated Sugarcane Bagasse and Ethanol Production. Waste Biomass Valor 13, 3085–3094 (2022). https://doi.org/10.1007/s12649-022-01685-1
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DOI: https://doi.org/10.1007/s12649-022-01685-1