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Nitrogen Reduction in Conjunction with Polyhydroxyalkanoates Production Using Mixed Enterobacter Strains

Abstract

In our previous study, textile wastewater (TW) treated with Enterobacter strain TS1L was improved dramatically after biological degradation. However, the nitrogen concentration of the treated water exceeded the threshold value in Thailand, resulting in eutrophication. Therefore, this study aimed to reduce the nitrogen content of treated TW (TTW) by re-treating it with Enterobacter. The nitrogen content of re-treated TW (RTW) was reduced from 110 to 33 mg/L at pH 7, 35 °C and 72 h of incubation with mixed Enterobacter strains. The properties of the RTW met the criteria of the Water Quality Standard (Thailand). Mixed strains accumulated medium-co-long chain length-polyhydroxyalkanoates (mcl-co-lcl-PHAs); 3(hydroxytetradecanoate-co-hydroxyhexadecanoate-co-hydroxyoctadecanoate); [3(HTD-co-HHD-co-HOD)] as also observed to be 14.79 (C14), 61.26 (C16) and 23.95 (C18) mol%, respectively, which was confirmed using gas chromatography–mass spectrometry (GC–MS) techniques. This is the first report of mixed Enterobacter strains with the ability to reduce nitrogen levels in conjunction with the production of mcl-co-lcl-PHAs, using TTW as substrate.

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Acknowledgements

The authors would like to acknowledge the Thailand Science Research and Innovation (TSRI) through the Royal Golden Jubilee PhD (RGJ-PHD) Program, Grant Number PHD/00073/2559, for RGJ-PHD. We also thank the Department of Chemistry, Faculty of Science, Thaksin University, Phatthalung Campus, Thailand, and Professor Dr Ken’ichiro Matsumoto.

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Correspondence to Kanokphorn Sangkharak.

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Rakkan, T., Chana, N. & Sangkharak, K. Nitrogen Reduction in Conjunction with Polyhydroxyalkanoates Production Using Mixed Enterobacter Strains. Waste Biomass Valor (2021). https://doi.org/10.1007/s12649-021-01601-z

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Keywords

  • Biomass
  • Enterobacter
  • Mcl-co-lcl-PHAs
  • Textile wastewater
  • Polyhydroxyalkanoates