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Effects of Dehairing Treatment on Gelatin Yield and Quality from Bovine Hides

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Abstract

Purpose

Hides are the by-product of slaughter houses which are mostly used for leather production. In Canada, the hides are either disposed of with other slaughter waste or sold at a very low price. Dehairing of hides is a prerequisite for either leather or gelatin production from it. Therefore, the effect of hide dehairing method on subsequent gelatin extraction and quality was investigated.

Methods

Bovine hides (BH) were dehaired using either 5% acetic acid (AA), 10% calcium hydroxide (CH), 0.02% keratinase (KTN), 2.5% papain (PP), or not at all (control; CT), with control BH subsequently treated with 5% AA (CTAA).

Results

Mean bovine hide gelatin (BHG) yields (dry basis) were 11.37%, 54.25%, 45.07%, 18.88% and 55.02% for CT, AA, CTAA, CH, and KTN, respectively. Gel strength was highest in AA followed by the CTAA, CT and CH and KTN treatments. The molecular weight (MW) distribution pattern showed that dehairing of BH with enzymes degraded the collagen extensively, increased proportions of low MW peptides that translated into low gel strength.

Conclusions

Acetic acid, which is extensively using in food industry, can be used to dehair BH as pre-treatment to extracting high quality gelatin.

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References

  1. Karim, A.A., Bhat, R.: Fish gelatin: properties, challenges, and prospects as an alternative to mammalian gelatins. Food Hydrocoll. 23, 563–576 (2009). https://doi.org/10.1016/j.foodhyd.2008.07.002

    Article  Google Scholar 

  2. Ahmed, M.A., Al-Kahtani, H.A., Jaswir, I., Tarboush, H.A., Ismail, E.A.: Extraction and characterization of gelatin from camel skin (potential halal gelatin) and production of gelatin nanoparticles. Saudi J Biol Sci 27, 1596–1601 (2020). https://doi.org/10.1016/j.sjbs.2020.03.022

    Article  Google Scholar 

  3. GMIA. Gelatin Handbook. Gelatin Manufacturers Institute of America. New York (2012) http://www.gelatin-gmia.com/ html/qanda.html [retrieved: 22.09.2012]

  4. Saravanabhavan, S., Rao, J.R., Nair, B.U., Ramasami, T.: An eco-efficient rationalized leather process. J. Chem. Technol. Biotechnol. 82, 971–984 (2007). https://doi.org/10.1002/jctb.1727

    Article  Google Scholar 

  5. Barbooti, M.M., Raouf, S.R., Al-Hamada, F.H.K.: Optimization of production of food grade gelatin from bovine hide wastes. Eng. Technol. 26, 240–253 (2008)

    Google Scholar 

  6. Riffel, A., Ortolan, S., Brandelli, A.: De–hairing activity of extracellular proteases produced by keratinolytic bacteria. J. Chem. Technol. Biotechnol. 78, 855–859 (2003). https://doi.org/10.1002/jctb.828

    Article  Google Scholar 

  7. De Souza, F.R., Gutterres, M.: Application of enzymes in leather processing: a comparison between chemical and coenzymatic processes. Braz. J. Chem. Eng. 29, 473–481 (2012). https://doi.org/10.1590/S0104-66322012000300004

    Article  Google Scholar 

  8. Traversa, E., Machado-Santelli, G.M., Velasco, M.V.R.: Histological evaluation of hair follicle due to papain’s depilatory effect. Int. J. Pharm 335, 163–166 (2007). https://doi.org/10.1016/j.ijpharm.2007.01.020

    Article  Google Scholar 

  9. Sompie, M., Surtijono, S.E., Pontoh, J.H.W., Lontaan, N.N.: The effects of acetic acid concentration and extraction temperature on physical and chemical properties of pig skin gelatin. Procedia Food Sci. 3, 383–388 (2015). https://doi.org/10.1016/j.profoo.2015.01.042

    Article  Google Scholar 

  10. Binsi, P.K., Shamasundar, B.A., Dileep, A.O., Badii, F., Howell, N.K.: Rheological and functional properties of gelatin from the skin of Big eye snapper (Priacanthus hamrur) fish: influence of gelatin on the gel-forming ability of fish mince. Food Hydrocoll. 23, 132–145 (2009). https://doi.org/10.1016/j.foodhyd.2007.12.004

    Article  Google Scholar 

  11. Snowden, J.: Collagen and method for producing same. International publication number WO 03/097694 A1. (2003) World Intellectual Property Organization International Bureau. https://patentscope.wipo.int.

  12. Schrieber, R., Gareis, H. (2007) From collagen to gelatin. In: Gelatine handbook: Theory and industrial practice. pp. 82–114. Weinheim Wiley–VCH

  13. AOAC: Official method of analysis of association of chemistry (2000) The Association of Official Analytical Chemists, Washington DC

  14. Shyni, K., Hema, G.S., Ninan, G., Mathew, S., Joshy, C.G., Lakshmanan, P.T.: Isolation and characterization of gelatin from the skins of skipjack tuna (Katsuwonus pelamis), dog shark (Scoliodon sorrakowah), and rohu (Labeo rohita). Food Hydrocoll. 39, 68–76 (2014). https://doi.org/10.1016/j.foodhyd.2013.12.008

    Article  Google Scholar 

  15. British Standards Institution: Methods for sampling and testing gelatin (physical and chemical methods). BSI, London (1975)

    Google Scholar 

  16. Roy, B.C., Das, C., Hong, H., Betti, M., Bruce, H.L.: Extraction and characterization of gelatin from bovine heart. Food Biosci. 20, 116–124 (2017). https://doi.org/10.1016/j.fbio.2017.09.004

    Article  Google Scholar 

  17. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685 (1970). https://doi.org/10.1038/227680a0

    Article  Google Scholar 

  18. Senthilvelan, T., Kanagaraj, J., Mandal, A.B.: Application of enzymes for dehairing of skins: cleaner leather processing. Clean Technol. Environ. Policy 14, 889–897 (2012). https://doi.org/10.1007/s10098-012-0458-5

    Article  Google Scholar 

  19. Wong, D.W.S.: Mechanism and theory in food chemistry. Van Nostrand Reinhold, New York (1989)

    Google Scholar 

  20. Liu, D.C., Lin, Y.K., Chen, M.T.: Optimum condition of extracting collagen from chicken feet and its characteristics. Asian-Australas. J. Anim. Sci. 14, 1638–1644 (2001). https://doi.org/10.5713/ajas.2001.1638

    Article  Google Scholar 

  21. Nalinanon, S., Benjakul, S., Visessanguan, W., Kishimura, H.: Improvement of gelatin extraction from bigeye snapper skin using pepsin-aided process in combination with protease inhibitor. Food Hydrocoll. 22, 615–622 (2008). https://doi.org/10.1016/j.foodhyd.2007.01.012

    Article  Google Scholar 

  22. Balti, R., Jridi, M., Sila, A., Souissi, N., Nedjar-Arroume, N., Guillochon, D., Nasri, M.: Extraction and functional properties of gelatin from the skin of cuttlefish (Sepia officinalis) using smooth hound crude acid protease-aided process. Food Hydrocoll. 25, 943–950 (2011). https://doi.org/10.1016/j.foodhyd.2010.09.005

    Article  Google Scholar 

  23. Jamilah, B., Harvinder, K.G.: Properties of gelatins from skins of fish–black tilapia (Oreochromis mossambicus) and red tilapia (Oreochromis nilotica). Food Chem. 77, 81–84 (2002). https://doi.org/10.1016/S0308-8146(01)00328-4

    Article  Google Scholar 

  24. Cole, C. G. B. Gelatin (2000) Encyclopedia of Food Science and Technology, Frederick, J. Francis (Eds) John Wiley & Sons New York pp 1183–1188

  25. Hafidz, R.M.: Chemical and functional properties of bovine and porcine skin gelatin. Int. Food Res. J. 18, 813–817 (2011)

    Google Scholar 

  26. Boran, G., Regenstein, J.M.: Optimization of gelatin extraction from silver carp skin. J. Food Sci. 74, E432–E441 (2009). https://doi.org/10.1111/j.1750-3841.2009.01328.x

    Article  Google Scholar 

  27. Kittiphattanabawon, P., Benjakul, S., Visessanguan, W., Shahidi, F.: Comparative study on characteristics of gelatin from the skins of brown banded bamboo shark and blacktip shark as affected by extraction conditions. Food Hydrocoll. 24, 164–171 (2010). https://doi.org/10.1016/j.foodhyd.2009.09.001

    Article  Google Scholar 

  28. Jridi, M., Nasri, R., Salem, R.B.S.B., Lassoued, I., Barkia, A., Nasri, M., Souissi, N.: Chemical and biophysical properties of gelatins extracted from the skin of octopus (Octopus vulgaris). LWT - Food Sci. Technol. 60, 881–889 (2015). https://doi.org/10.1016/j.lwt.2014.10.057

    Article  Google Scholar 

  29. Arnesen, J.A., Gildberg, A.: Preparation and characterisation of gelatine from the skin of harp seal (Phoca groendlandica). Bioresour. Technol. 82, 191–194 (2002). https://doi.org/10.1016/S0960-8524(01)00164-X

    Article  Google Scholar 

  30. Muyonga, J.H., Cole, C.G.B., Duodu, K.G.: Extraction and physico-chemical characterisation of Nile perch (Lates niloticus) skin and bone gelatin. Food Hydrocoll. 18, 581–592 (2004). https://doi.org/10.1016/j.foodhyd.2003.08.009

    Article  Google Scholar 

  31. Bkhairia, I., Mhamdi, S., Jridi, M., Nasri, M.: New acidic proteases from Liza aurata viscera: Characterization and application in gelatin production. Int. J. Biol. Macromol. 92, 533–542 (2016). https://doi.org/10.1016/j.ijbiomac.2016.07.063

    Article  Google Scholar 

  32. Binsi, P.K., Nayak, N., Sarkar, P.C., Joshy, C.G., Ninan, G., Ravishankar, C.N.: Gelation and thermal characteristics of microwave extracted fish gelatin–natural gum composite gels. J. Food Sci. Technol. 54, 518–530 (2017). https://doi.org/10.1007/s13197-017-2496-9

    Article  Google Scholar 

  33. Nagarajan, M., Benjakul, S., Prodpran, T., Songtipy, P., Kishimura, H.: Characteristics and functional properties of gelatin from splendid squid (Loligo formosana) skin as affected by extraction temperatures. Food Hydrocoll. 29, 389–397 (2012). https://doi.org/10.1016/j.foodhyd.2012.04.001

    Article  Google Scholar 

  34. Jamilah, B., Tan, K.W., Umi-Hartina, M.R., Azizah, A.: Gelatins from three cultured freshwater fish skins obtained by liming process. Food Hydrocoll. 25, 1256–1260 (2011). https://doi.org/10.1016/j.foodhyd.2010.11.023

    Article  Google Scholar 

  35. Avena-Bustillos, R., Olsen, C., Olson, D., Chiou, B., Yee, E., Bechtel, P., Mchugh, T.: Water vapor permeability of mammalian and fish gelatin films. J. Food Sci. 71, E202–E207 (2006). https://doi.org/10.1111/j.1750-3841.2006.00016.x

    Article  Google Scholar 

  36. Zuniga, R.N., Aguilera, J.M.: Structure fracture relationships in gas-filled gelatin gels. Food Hydrocoll. 23, 1351–1357 (2009). https://doi.org/10.1016/j.foodhyd.2008.11.012

    Article  Google Scholar 

  37. Ahmad, M., Benjakul, S.: Characteristics of gelatin from the skin of unicorn leatherjacket (Aluterus monoceros) as influenced by acid pre-treatment and extraction time. Food Hydrocoll. 25, 381–388 (2011). https://doi.org/10.1016/j.foodhyd.2010.07.004

    Article  Google Scholar 

  38. Rawdkuen, S., Thitipramote, N., Benjakul, S.: Preparation and functional characterization of fish skin gelatin and comparison with commercial gelatin. Int. J. Food Sci. Technol. 48, 1093–1102 (2013). https://doi.org/10.1111/ijfs.12067

    Article  Google Scholar 

  39. Wasswa, J., Tang, J., Gu, X.: Utilization of fish processing by products in the gelatin industry. Food Rev. Int. 23, 159–174 (2007). https://doi.org/10.1080/87559120701225029

    Article  Google Scholar 

  40. Kaewruang, P., Benjakul, S., Prodpran, T.: Effect of phosphorylation on gel properties of gelatin from the skin of unicorn leatherjacket. Food Hydrocoll. 35, 694–699 (2014). https://doi.org/10.1016/j.foodhyd.2013.08.017

    Article  Google Scholar 

  41. Ninan, G., Jose, J., Abubacker, Z.: Preparation and characterization of gelatin extracted from the skins of rohu (Labeo rohita) and common carp (Cyprinus carpio). J. Food Process. Preserv. 35, 143–162 (2011). https://doi.org/10.1111/j.1745-4549.2009.00467.x

    Article  Google Scholar 

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Acknowledgements

The authors would like to thanks Alberta Livestock and Meat Agency (Grant No. 2011R030R) for financial support.

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Authors and Affiliations

  1. Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada

    Bimol C. Roy, Chamali Das, Hui Hong, Mirko Betti & Heather L. Bruce

  2. College of Food Science and Nutritional Engineering, China Agricultural University, Haidian, Beijing, 100091, China

    Hui Hong

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Contributions

Bimol C. Roy and Chamali Das jointly performed assays and laboratory work, and Bimol C. Roy wrote the manuscript. Heather L. Bruce performed experimental design, statistical analysis, and manuscript review and editing. Hui Hong assisted with FTIR data analyses and manuscript editing and review. Mirko Betti assisted with the Rheometer run, editing and review of the manuscript.

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Correspondence to Bimol C. Roy.

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Roy, B.C., Das, C., Hong, H. et al. Effects of Dehairing Treatment on Gelatin Yield and Quality from Bovine Hides. Waste Biomass Valor 13, 831–843 (2022). https://doi.org/10.1007/s12649-021-01582-z

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