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Production and Recovery of Exo-polygalacturonase from Umbu (Spondias tuberosa) Residue

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Abstract

The objective of the present work was to produce exo-polygalacturonase by solid-state fermentation of the dehydrated peels of umbu (Spondias tuberosa) using Aspergillus niger CCT 0916 and then, using a biphasic aqueous system, recover the enzyme. The peels were dehydrated at 55 °C to obtain the dried residue and then used to produce the exo-polygalacturonase using Aspergillus niger CCT 0916 and solid-state fermentation. During the 72 h fermentation, the pH, reducing sugars, and the exo-polygalacturonase activity were tested. Then the extracted enzyme was recovered with a biphasic aqueous system composed of PEG/potassium phosphate. The umbu dried residue showed a maximum peak of enzymatic activity with values of 17.30 U/g after 44 h of fermentation. The enzyme extraction using the dried umbu residue yielded a maximum PGA (Activity polygalacturonase) of 32.59 U/g. The biphasic aqueous system composed of PEG8000 and potassium phosphate salts yielded the highest recovery of 97.14%.

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Acknowledgements

The authors acknowledge the State University of Paraíba and the Department of Chemistry.

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Authors and Affiliations

  1. Department of Chemistry, State University of Paraíba, Campina Grande, PB, Brazil

    Ângela Maria Santiago & Pablícia Oliveira Galdino

  2. Department of Chemical Engineering, Federal University of Campina Grande, Campina Grande, PB, Brazil

    Líbia de Sousa Conrado Oliveira & Paloma Lima de Oliveira

  3. Department of Chemical Engineering, Federal University of Rio Grande Do Norte, Natal, RN, Brazil

    Raphael Lucas Jacinto Almeida & Newton Carlos Santos

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  1. Ângela Maria Santiago
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ÂMS: Conceptualization, Methodology, Writing- Original draft preparation, Funding acquisition. LSCO: Funding acquisition, Reviewing and Editing PLO: Data Curation. RLJA: Statistic, Visualization. NCS: Software, Statistic. POG: Supervision Writing.

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Santiago, Â.M., de Sousa Conrado Oliveira, L., de Oliveira, P.L. et al. Production and Recovery of Exo-polygalacturonase from Umbu (Spondias tuberosa) Residue. Waste Biomass Valor 13, 1101–1115 (2022). https://doi.org/10.1007/s12649-021-01551-6

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