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Effect of Oleic Acid on Neuronal Zinc Signals

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Abstract

The culture of olive trees has been spreading worldwide with huge areas being used for this purpose. This activity, in addiction to that of the olive oil industries, has resulted in the formation of much biomass and effluents contaminated with high concentrations of organic compounds. Oleic acid (OA) is a monounsaturated compound, abundant in olive oil and almonds and used for the production of energy, cosmetics, nutritional products and pharmaceuticals, with many nutritional and medicinal benefits. However, it may be present in toxic amounts in industrial effluents of the food sector. There is an increasing incidence of neurological diseases associated with excessive neuronal zinc release and also with the production of reactive oxygen species (ROS), mainly in mitochondria. This work addresses the impact of oleic acid in neuronal zinc movements and compares OA induced zinc and ROS signals. The measurements were performed using the fluorescent zinc and ROS indicators Newport Green and H2DCFDA, respectively, and oleic acid concentrations in the 10 μM – 100 μM range. The measured zinc and ROS signals, which include autofluorescence, were both enhanced in the presence of the higher concentrations of oleic acid. In general, their amplitude increased with the OA concentration but, upon washout, the zinc changes were reversible while the ROS signals were maintained. These results suggest that there is a correlation between both types of cellular changes.

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Acknowledgements

We thank CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal, for providing the rat brains. Work funded by strategic Project UID/NEU/04539/2013.

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Authors and Affiliations

  1. Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3004-516, Coimbra, Portugal

    J. L. Alves & A. S. C. Figueira

  2. CIEPQPF – Chemical Engineering Processes and Forest Products Research Center, Department of Chemical Engineering, University of Coimbra, Rua Sílvio Lima, 3030-790, Coimbra, Portugal

    I. L. Lopes & R. M. Quinta-Ferreira

  3. LIP – Laboratory of Instrumentation and Experimental Particles Physics and Physics Department, University of Coimbra, P-3004-516, Coimbra, Portugal

    P. J. Mendes

  4. Center for Neurosciences and Cell Biology and Physics Department, University of Coimbra, Rua Larga, 3004-516, Coimbra, Portugal

    M. E. Quinta-Ferreira

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  1. J. L. Alves
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  5. R. M. Quinta-Ferreira
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  6. M. E. Quinta-Ferreira
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Correspondence to J. L. Alves.

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Alves, J.L., Figueira, A.S.C., Lopes, I.L. et al. Effect of Oleic Acid on Neuronal Zinc Signals. Waste Biomass Valor 12, 3019–3025 (2021). https://doi.org/10.1007/s12649-021-01350-z

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  • DOI: https://doi.org/10.1007/s12649-021-01350-z

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