Abstract
The high cost of oleaginous microalgae harvesting is a major factor limiting the industrialization of microalgae biodiesel. In order to obtain an efficient, environmentally friendly and cost-effective method for harvesting microalgae, this study used Citrobacter freundii (No. W4) and Mucor circinelloides for the harvest of oil-bearing microalgae Chlorella pyrenoidosa. The effects of flocculation conditions, including the initial pH, bacterial dosage, fungal dosage and glucose dosage were investigated, respectively. According to the results of response surface optimization, when the initial pH was 7.0, bacteria: microalgae ratio was 1.6:1, microalgae: fungi ratio was 333:1, the glucose concentration was 1.47 g/L, the flocculation efficiency of the actual sewage cultured C. pyrenoidosa attained 97.45%. The results showed that bacteria and fungi had a good potential for the harvest of microalgae.
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This work was supported by National Natural Science Foundation of China (No. 51878215), China Postdoctoral Science Foundation (2019M661265), Natural Science Foundation of Guangdong Province, China (2018A030313185) and Shenzhen Science and Technology Innovation Project (KJYY20171011144235970, JCYJ20170307150223308).
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Jiang, J., Jin, W., Tu, R. et al. Harvesting of Microalgae Chlorella pyrenoidosa by Bio-flocculation with Bacteria and Filamentous Fungi. Waste Biomass Valor 12, 145–154 (2021). https://doi.org/10.1007/s12649-020-00979-6
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DOI: https://doi.org/10.1007/s12649-020-00979-6