Microbial, Nutritional, and Antioxidant Stability of Fruit and Vegetables Discards Treated with Sodium Metabisulfite During Aerobic and Anaerobic Storage

Abstract

Fruits and vegetables are a rich source of natural antioxidants; therefore their discards can be viewed as a functional feed ingredient in animal nutrition. The aim of present study was to examine the effects of sodium metabisulfite (SMB) on microbial, nutritional, and antioxidant stability of fruit and vegetable discards (FVD) under laboratory- and large-scale conditions. Initially, FVD were mixed without or with 6 g SMB/kg biomass, aerobically challenged for 7 days, and then stored anaerobically up to 28 days. Under both aerobic and anaerobic conditions, negligible loss of the nutrient constituents was evident in SMB-treated FVD. Conversely, the rapid rise in the microbial population of FVD (without SMB) resulted in biomass deterioration and substantial dry matter loss and sugar exhaustion. Although the prolonged storage of SMB-treated FVD resulted in the moderate loss of carotenoids, total phenolics and DPPH radical scavenging activity slightly changed. Overall, a series of laboratory- and large-scale experiments demonstrated the effectiveness of SMB in conserving the nutrient constituents and the antioxidant capacity of FVD under aerobic and anaerobic storage, which might enable a viable route to the effective utilization of these discards as a functional ingredient for animal feed applications.

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References

  1. 1.

    Sagar, N.A., Pareek, S., Sharma, S., Yahia, E.M., Lobo, M.G.: Fruit and vegetable waste: bioactive compounds, their extraction, and possible utilization. Compr. Rev. Food Sci. Food Saf. 17, 512–531 (2018)

    Google Scholar 

  2. 2.

    Angulo, J., Mahecha, L., Yepes, S.A., Yepes, A.M., Bustamante, G., Jaramillo, H., Valencia, E., Villamil, T., Gallo, J.: Nutritional evaluation of fruit and vegetable waste as feedstuff for diets of lactating Holstein cows. J. Environ Manage. 95, 210–214 (2012)

    Google Scholar 

  3. 3.

    Romero-Huelva, M., Ramos-Morales, E., Molina-Alcaide, E.: Nutrient utilization, ruminal fermentation, microbial abundances, and milk yield and composition in dairy goats fed diets including tomato and cucumber waste fruits. J. Dairy Sci. 95, 6015–6026 (2012)

    Google Scholar 

  4. 4.

    Karam, M.C., Petit, J., Zimmer, D., Djantou, E.B., Scher, J.: Effects of drying and grinding in production of fruit and vegetable powders: A review. J. Food Eng. 188, 32–49 (2016)

    Google Scholar 

  5. 5.

    Ahmadi, F., Lee, Y.H., Lee, W.H., Oh, Y.K., Park, K., Kwak, W.S.: Preservation of fruit and vegetable discards with sodium metabisulfite. J. Environ. Manage. 224, 113–121 (2018)

    Google Scholar 

  6. 6.

    Arvanitoyannis, I.S., 2008. Waste management for the food industries. Academic Press – Elsevier Ltd., Oxford, UK.

    Google Scholar 

  7. 7.

    Mirabella, N., Castellani, V., Sala, S.: Current options for the valorization of food manufacturing waste: a review. J. Clean. Prod. 65, 28–41 (2014)

    Google Scholar 

  8. 8.

    Ahmadi, F., Lee, Y.H., Lee, W.H., Oh, Y.K., Park, K., Kwak, W.S.: Long-term anaerobic conservation of fruit and vegetable discards without or with moisture adjustment after aerobic preservation with sodium metabisulfite. Waste Manage. 87, 258–267 (2019)

    Google Scholar 

  9. 9.

    Weinberg, Z.G., Muck, R.: New trends and opportunities in the development and use of inoculants for silage. FEMS Microbiol. Rev. 19, 53–68 (1996)

    Google Scholar 

  10. 10.

    Bacenetti, J., Fusi, A.: The environmental burdens of maize silage production: influence of different ensiling techniques. Anim. Feed Sci. Technol. 204, 88–98 (2015)

    Google Scholar 

  11. 11.

    Eliyahu, D., Shaani, Y., Yosef, E., Ben-Meir, Y., Nikbachat, M., Solomon, R., Mabjeesh, S.J., Weinberg, Z.G., Miron, J.: Effect of ensiling pomegranate pulp with solid additives on chemical composition, intake and digestibility by sheep. Small Rumin. Res. 131, 93–98 (2015)

    Google Scholar 

  12. 12.

    Hafner, S.D., Howard, C., Muck, R.E., Franco, R.B., Montes, F., Green, P.G., Mitloehner, F., Trabue, S.L., Rotz, C.A.: Emission of volatile organic compounds from silage: Compounds, sources, and implications. Atmos. Environ. 77, 827–839 (2013)

    Google Scholar 

  13. 13.

    Robinson, P.H., Swanepoel, N., Heguy, J.M., Price, T., Meyer, D.M.: ‘Shrink’ losses in commercially sized corn silage piles: quantifying total losses and where they occur. Sci. Total Environ. 542, 530–539 (2016)

    Google Scholar 

  14. 14.

    Borreani, G., Tabacco, E., Schmidt, R., Holmes, B., Muck, R.: Silage review: Factors affecting dry matter and quality losses in silages. J. Dairy Sci. 101, 3952–3979 (2018)

    Google Scholar 

  15. 15.

    Mahmoud, A.A.T., Hassan, G.M., Hassan, A.M.S., Latif, A.K.M.A., Ramadan, M.F.: Demonstrating adverse effects of a common food additive (sodium sulfite) on biochemical, cytological and histopathological parameters in tissues of albino Wister rats. Eur. J. Integr. Med. 7, 234–242 (2015)

    Google Scholar 

  16. 16.

    Bratzler, J., Cowan, R., Swift, R.: Grass silage preservation with sodium metabisulfite. J. Anim. Sci. 15, 163–176 (1956)

    Google Scholar 

  17. 17.

    Cowan, R.L., Bratzler, J.W., Keck Jr, E., Swift, R.W., Alderman, G., Washko, J.B., 1956. Further experiments with sodium bisulfite as a preservative for grass silage. J. Anim. Sci. 15, 1188–1198 (1956).

    Google Scholar 

  18. 18.

    Truong, H.H., Neilson, K.A., McInerney, B.V., Khoddami, A., Roberts, T.H., Liu, S.Y., Selle, P.H.: Sodium metabisulphite enhances energy utilisation in broiler chickens offered sorghum-based diets with five different grain varieties. Anim. Feed Sci. Technol. 219, 159–174 (2016)

    Google Scholar 

  19. 19.

    Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P., Smith, F.: Colorimetric method for determination of sugars and related substances. Anal. Chem. 28, 350–356 (1956)

    Google Scholar 

  20. 20.

    Chaney, A.L., Marbach, E.P.: Modified reagents for determination of urea and ammonia. Clin. Chem. 8, 130–132 (1962)

    Google Scholar 

  21. 21.

    Porter, M., Murray, R.: The volatility of components of grass silage on oven drying and the inter-relationship between dry-matter content estimated by different analytical methods. Grass and Forage Sci. 56, 405–411 (2001)

    Google Scholar 

  22. 22.

    Weißbach, F., Strubelt, C.: Correcting the dry matter content of maize silages as a substrate for biogas production. Landtechnik 63, 82–83 (2008)

    Google Scholar 

  23. 23.

    Barker, S.B., Summerson, W.H.: The colorimetric determination of lactic acid in biological material. J. Biol. Chem. 138, 535–554 (1941)

    Google Scholar 

  24. 24.

    Chandrasekara, A., Shahidi, F.: Content of insoluble bound phenolics in millets and their contribution to antioxidant capacity. J. Agric. Food Chem. 58, 6706–6714 (2010)

    Google Scholar 

  25. 25.

    Singleton, V.L., Rossi, J.A.: Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Viticult. 16, 144–158 (1965)

    Google Scholar 

  26. 26.

    Silva, K.D.R.R., Sirasa, M.S.F.: Antioxidant properties of selected fruit cultivars grown in Sri Lanka. Food Chem. 238, 203–208 (2018)

    Google Scholar 

  27. 27.

    de Carvalho, L.M.J., Gomes, P.B., de Oliveira Godoy, R.L., Pacheco, S., do Monte, P.H.F., de Carvalho, J.L.V., Nutti, M.R., Neves, A.C.L., Vieira, A.C.R.A., Ramos, S.R.R., : Total carotenoid content, α-carotene and β-carotene, of landrace pumpkins (Cucurbita moschata Duch): A preliminary study. Food Res. Int. 47, 337–340 (2012)

    Google Scholar 

  28. 28.

    Brand-Williams, W., Cuvelier, M., Berset, C.: Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci. Technol. 28, 25–30 (1995)

    Google Scholar 

  29. 29.

    Leong, L., Shui, G.: An investigation of antioxidant capacity of fruits in Singapore markets. Food Chem. 76, 69–75 (2002)

    Google Scholar 

  30. 30.

    Kim, D.O., Jeong, S.W., Lee, C.Y.: Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem. 81, 321–326 (2003)

    Google Scholar 

  31. 31.

    Institute., S. SAS User’s Guide. Version 9.1. SAS Institute Inc., Cary, NC. 2003.

    Google Scholar 

  32. 32.

    McDonald, P., Henderson, N., Heron, S.: The biochemistry of silage. Cambrian Printers Ltd., Aberystwyth, UK (1991)

    Google Scholar 

  33. 33.

    Spoelstra, S.F., Courtin, M.G., Vanbeers, J.A.C.: Acetic-acid bacteria can initiate aerobic deterioration of whole crop maize silage. J. Agric. Sci. 111, 127–132 (1988)

    Google Scholar 

  34. 34.

    Brüning, D., Gerlach, K., Weiß, K., Südekum, K.H.: Effect of compaction, delayed sealing and aerobic exposure on maize silage quality and on formation of volatile organic compounds. Grass and Forage Sci. 73, 53–66 (2018)

    Google Scholar 

  35. 35.

    Ashbell, G., Pahlow, G., Dinter, B., Weinberg, Z.: Dynamics of orange peel fermentation during ensilage. J. Appl. Microbiol. 63, 275–279 (1987)

    Google Scholar 

  36. 36.

    Driehuis, F., Elferink, S., Spoelstra, S.: Anaerobic lactic acid degradation during ensilage of whole crop maize inoculated with Lactobacillus buchneri inhibits yeast growth and improves aerobic stability. J. Appl. Microbiol. 87, 583–594 (1999)

    Google Scholar 

  37. 37.

    Divol, B., du Toit, M., Duckitt, E.: Surviving in the presence of sulphur dioxide: strategies developed by wine yeasts. Appl. Microbiol. Biotechnol. 95, 601–613 (2012)

    Google Scholar 

  38. 38.

    Alderman, G., Cowan, R., Bratzler, J., Swift, R.: Some chemical characteristics of grass and legume silage made with sodium metabisulfite. J. Dairy Sci. 38, 805–810 (1955)

    Google Scholar 

  39. 39.

    Bolin, H.R., Jackson, R.: Factors affecting sulfur dioxide binding in dried apples and apricots. J. Food Process. Pres. 9, 25–34 (1985)

    Google Scholar 

  40. 40.

    Ough, C.S., Were, L., 2005. Sulfur dioxide and sulfites. In Davidson, P.M., Sofos, J.N., Branen, A., (eds), Antimicrobials in Foods, Boca Raton, FL: CRC Press.

    Google Scholar 

  41. 41.

    Everette, J.D., Bryant, Q.M., Green, A.M., Abbey, Y.A., Wangila, G.W., Walker, R.B.: A thorough study of reactivity of various compound classes towards the Folin-Ciocalteu reagent. J. Agric. Food Chem. 58, 8139–8144 (2010)

    Google Scholar 

  42. 42.

    Cisneros-Zevallos, L.: The use of controlled postharvest abiotic stresses as a tool for enhancing the nutraceutical content and adding-value of fresh fruits and vegetables. J. Food Sci. 68, 1560–1565 (2003)

    Google Scholar 

  43. 43.

    Kays, S.J.: Stress in harvested products. In: Kays, S.J. (ed.) Postharvest physiology of perishable plant products, pp. 335–407. Exon Press, Athens, GA (1997)

    Google Scholar 

  44. 44.

    Reyes, L.F., Villarreal, J.E., Cisneros-Zevallos, L.: The increase in antioxidant capacity after wounding depends on the type of fruit or vegetable tissue. Food Chem. 101, 1254–1262 (2007)

    Google Scholar 

  45. 45.

    Reyes, L.F., Cisneros-Zevallos, L.: Wounding stress increases the phenolic content and antioxidant capacity of purple-flesh potatoes. J. Agric. Food Chem. 51, 5296–5300 (2003)

    Google Scholar 

  46. 46.

    Sánchez-Moreno, C., Larrauri, J.A., Saura-Calixto, F.: Free radical scavenging capacity of selected red, rose and white wines. J. Sci. Food Agric. 79, 1301–1304 (1999)

    Google Scholar 

  47. 47.

    Wijngaard, H.H., Rößle, C., Brunton, N.: A survey of Irish fruit and vegetable waste and by-products as a source of polyphenolic antioxidants. Food Chem. 116, 202–207 (2009)

    Google Scholar 

  48. 48.

    Chen, J.P., Tai, C.Y., Chen, B.H.: Effects of different drying treatments on the stability of carotenoids in Taiwanese mango (Mangifera indica L.). Food Chem. 100, 1005–1010 (2007)

    Google Scholar 

  49. 49.

    Aydin, E., Gocmen, D.: The influences of drying method and metabisulfite pre-treatment on the color, functional properties and phenolic acids contents and bioaccessibility of pumpkin flour. LWT-Food Sci. Technol. 60, 385–392 (2015)

    Google Scholar 

  50. 50.

    Dennison, D.B., Kirk, J.R.: Effect of trace metal fortification on the storage stability of ascorbic acid in a dehydrated model food system. J. Food Sci. 47, 1198–1200 (1982)

    Google Scholar 

  51. 51.

    Kleinschmit, D., Schmidt, R., Kung, L.: The effects of various antifungal additives on the fermentation and aerobic stability of corn silage. J. Dairy Sci. 88, 2130–2139 (2005)

    Google Scholar 

  52. 52.

    Ramesh, M.N., Wolf, W., Tevini, D., Jung, G.: Influence of processing parameters on the drying of spice paprika. J. Food Eng. 49, 63–72 (2001)

    Google Scholar 

  53. 53.

    Smith, W.C., Campbell, I.L.: Sodium metabisulphite as an additive in silage making. New Zeal. J. Agr. Res. 3, 1027–1037 (1960)

    Google Scholar 

  54. 54.

    Meiske, J.C., Prouty, R.M., Schuman, L.M., Scaletti, J.V.: Effect of sodium bisulfite additions to corn silages. J. Anim. Sci. 24, 705–710 (1965)

    Google Scholar 

  55. 55.

    Zhao, Y.P., Chang, K.C.: Sulfite and starch affect color and carotenoids of dehydrated carrots (Daucus carota) during storage. J. Food Sci. 60, 324–326 (1995)

    Google Scholar 

  56. 56.

    Gardner, P.T., White, T.A., McPhail, D.B., Duthie, G.G.: The relative contributions of vitamin C, carotenoids and phenolics to the antioxidant potential of fruit juices. Food Chem. 68, 471–474 (2000)

    Google Scholar 

  57. 57.

    Vibhakara, H.S.J., Gupta, D.K.D., Jayaraman, K.S., Mohan, M.S.: Development of a high-moisture shelf-stable grated carrot product using hurdle technology. J. Food Process. Preserv. 30, 134–144 (2006)

    Google Scholar 

  58. 58.

    Hymavathi, T.V., Khader, V.: Carotene, ascorbic acid and sugar content of vacuum dehydrated ripe mango powders stored in flexible packaging material. J. Food Compos. Anal. 18, 181–192 (2005)

    Google Scholar 

  59. 59.

    Mir, M.A., Nath, N.: Storage changes in fortified mango bars. J. Food Sci. Technol. 30, 279–287 (1993)

    Google Scholar 

  60. 60.

    Kalač, P.: The effects of silage feeding on some sensory and health attributes of cow’s milk: A review. Food Chem. 125, 307–317 (2011)

    Google Scholar 

  61. 61.

    Rodriguez-Amaya, D.: Changes in carotenoids during processing and storage of foods. Arch. Latinoam. Nutr. 49, 38–47 (1999)

    Google Scholar 

  62. 62.

    Baloch, W.A., Khan, S., Baloch, A.K.: Influence of chemical additives on the stability of dried tomato powder. Int. J. Food Sci. Technol. 32, 117–120 (1997)

    Google Scholar 

  63. 63.

    Nadeem, M., Ubaid, N., Qureshi, T.M., Munir, M., Mehmood, A.: Effect of ultrasound and chemical treatment on total phenol, flavonoids and antioxidant properties on carrot-grape juice blend during storage. Ultrason. Sonochem. 45, 1–6 (2018)

    Google Scholar 

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Acknowledgement

This study was performed with the financial support of the “Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ012507032019)” Rural Development Administration, Republic of Korea. This study was also supported by Konkuk University.

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Ahmadi, F., Lee, W.H., Oh, YK. et al. Microbial, Nutritional, and Antioxidant Stability of Fruit and Vegetables Discards Treated with Sodium Metabisulfite During Aerobic and Anaerobic Storage. Waste Biomass Valor 12, 347–357 (2021). https://doi.org/10.1007/s12649-020-00968-9

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Keywords

  • Antioxidant capacity
  • Carotenoid
  • Fruit and vegetable waste
  • Nutrient composition
  • Phenolics