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Optimization of Enzymatic Pretreatments to Obtain Fermentable Sugars from Fruit and Vegetable Waste

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Abstract

Biofuels production from organic waste requires an efficient hydrolysis to produce fermentable sugars. Physicochemical and biological pretreatment corresponds to good alternative to improve biofuel yield. Nonetheless, physical treatments can result in a negative energy balance and chemical treatments can generate inhibitors for a fermentative process, as well as difficulties in the recycling of organic matter. Thus, enzymatic pretreatments lead to fast and ecofriendly processes for conversion of waste biomass into monomeric units. In this work, fruit and vegetable wastes were hydrolyzed applying three types of enzymatic complexes: Viscozyme® L and a mixture of Multifect® B and Naturalzyme 40 XLTM. Production of reducing sugars as a temperature function and enzyme concentration was optimized by response surface analysis. Enzymatic complexes revealed high hydrolysis yield. Viscozyme® L application is highlighted; obtaining a hydrolysis higher than with the mixture of Multifect® B and Naturalzyme 40 XLTM (80% and 60% respectively) and being a promising treatment for the development of an efficient saccharification process of fruit and vegetable wastes. The optimum application conditions for Viscozyme® L were of 0.24 ppm and 40 °C and were validated with an error rate of 5%. For the mixture of Multifect® B and Naturalzyme 40 XLTM with Pectinase/Polygalacturonase activity, an optimum 0.93 ppm and 41 °C was found; these were validated with an error of 6%. Therefore, cellulases, xylanases and hemicellulases (Viscozyme® L) assured the hydrolysis of food wastes obtaining better available sugars for successive fermentative processes.

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Acknowledgements

Authors acknowledge to Universidad Nacional de Colombia for funding the project "Evaluation of enzymatic hydrolysis of organic waste to produce biofuels" within which this work was carried out. Hermes Code: 36096, through the "National Call for Projects to Strengthen Research, Creation, and Innovation of Universidad Nacional de Colombia 2016–2018".

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  1. Department of Agricultural Engineering and Food, Faculty of Agricultural Sciences, Universidad Nacional de Colombia - Sede Medellin, Carrera 65 N° 59A – 110, Medellin, Colombia

    Michael A. Cabas Candama, Sara Duque Martinez & Edith M. Cadena Chamorro

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  1. Michael A. Cabas Candama
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Correspondence to Sara Duque Martinez.

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Cabas Candama, M.A., Duque Martinez, S. & Cadena Chamorro, E.M. Optimization of Enzymatic Pretreatments to Obtain Fermentable Sugars from Fruit and Vegetable Waste. Waste Biomass Valor 11, 5991–6002 (2020). https://doi.org/10.1007/s12649-019-00821-8

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