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Potential of Chestnut Wastes for Cosmetics and Pharmaceutical Applications

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Abstract

The aqueous extraction of Castanea sativa underutilized parts (leaves, burs, husks) was proposed to obtain different soluble and insoluble fractions. The optimum extraction and concentration conditions were selected based on previously published studies. The extracts from leaves and husks were concentrated by membranes and those from burs were refined by adsorption–desorption to concentrate the active phenolic compounds showing radical scavenging capacity. A preliminary screening was made in order to explore the extracts’ pharmaceutical potential, evaluating the cell viability against representative human tumoral cells and the potential as a cosmetic ingredient was also evaluated for their inhibitory activities against collagenase, elastase and tyrosinase. In addition, chestnut husk ashes were used as an alternative source for potash production suitable to formulate potassium soaps with interesting mechanical properties.

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Abbreviations

A549:

Epithelial lung adenocarcinoma cells

ABTS:

2,2′-Azinobis (3-ethyl-benzothiazoline-6-sulfonate)

BT474:

Breast cancer HER2+ cells

CB:

Chestnut burs

CBCE:

Chestnut burs concentrated extract

CBE:

Chestnut burs extract

CH:

Chestnut leaves

CHACE:

Chestnut husks ashes concentrated of extract

CHAE:

Chestnut husks ashes of extract

CHCE:

Chestnut husks concentrated extract

CHE:

Chestnut husks extract

CLCE:

Chestnut leaves concentrated extract

CLE:

Chestnut leaves extract

CsL:

Castanea sativa Leaves

DMSO:

Dimethyl sulfoxide

ECM:

Enzymes of the extracellular matrix

EGCG:

Epigallocatechin galate

GAE:

Gallic acid equivalent

HCT-116:

Colon carcinoma cells

HEL:

Eritroleukemia cells

HL60:

Acute promyelocytic leukemia cells

ICP-MS:

Inductively coupled plasma mass spectrometry

KG1:

Acute myeloid leukemia cells

L-DOPA:

3,4-Dihydroxy-l-phenylalanine

MCF7:

Breast cancer ER+ cells

MDA-MB-231:

Breast cancer cells

MMP-1:

Human recombinant collagenase

MMPs:

Matrix metalloproteinases

MTT:

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

NCIH460:

Lung cancer cells

PBS:

Phosphate buffer saline

PSN1:

Pancreatic adenocarcinoma cells

ROS:

Reactive oxygen species

SKBr3:

Breast cancer HER2+ cells

T98G:

Caucasian human glioblastoma cells

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Acknowledgements

The authors are grateful to the Xunta de Galicia (FEADER 2016/23B and CINBIO). Both projects were partially funded by the FEDER Program of the European Union (“Unha maneira de facer Europa”). M.D.T. thanks Spanish Ministry of Economy and Competitiveness for her postdoctoral grant (IJCI-2016-27535). N.F.F. and S.G. thank CINBIO (Centro singular de investigación de Galicia accreditation 2016–2019).

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Vigo (Campus Ourense), Edificio Politécnico, As Lagoas, 32004, Ourense, Spain

    N. Flórez-Fernández, M. D. Torres, S. Gómez & H. Domínguez

  2. Amarelante Sociedade Cooperativa Galega. A Corredoira, 32781, Manzaneda, Ourense, Spain

    S. Couso

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  1. N. Flórez-Fernández
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Correspondence to M. D. Torres.

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Flórez-Fernández, N., Torres, M.D., Gómez, S. et al. Potential of Chestnut Wastes for Cosmetics and Pharmaceutical Applications. Waste Biomass Valor 11, 4721–4730 (2020). https://doi.org/10.1007/s12649-019-00784-w

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