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Waste Euryale ferox Salisb. Leaves as a Potential Source of Anthocyanins: Extraction Optimization, Identification and Antioxidant Activities Evaluation

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Abstract

In this study, the waste leaves of Euryale ferox Salisb. as a potential source of anthocyanins was investigated. Ultrasound-assisted technique with Box–Behnken design was applied for optimization of the anthocyanins extraction from Euryale ferox leaves. As a result, the optimal extraction condition was as follows: ultrasound temperature was 40 °C, ultrasound time was 38 min, ethanol concentration was 62% (v/v) and solvent-to-solid ratio was 27 mL/g. And the maximum yield of anthocyanins was 281.56 ± 3.02 mg/100 g under the optimum extraction parameters. The experimental data was close to the predicted anthocyanins yields of 289.45 mg/100 g and significantly higher than the yield of 181.81 ± 1.94 mg/100 g obtained by conventional solvent extraction. Moreover, the result of HPLC-QTOF-MS/MS analysis identified nineteen compounds in the purified anthocyanins extracts. And the anthocyanins extracts exhibited high scavenging activities on α,α-diphenyl-β-picrylhydrazyl and 2,2′-azinobis- (3-ethylbenzthiazoline-6-sulphonic acid) radicals. Results implied that Ultrasound-assisted extraction was a suitable method for anthocyanins extraction from Euryale ferox leaves with good antioxidant activities, and Euryale ferox leaves could be a potential source of natural anthocyanins used as antioxidant in functional food or medicine.

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Acknowledgements

This work was supported by National Key Technology Research and Development Program of the Ministry of Science and Technology of China during the “12th Five-Year Plan” (2011BAI04B06), the Funds of Innovative Research Team in Research on Resource Chemistry of Traditional Chinese Medicine of Jiangsu High Education Institution of China (2011), and “Six Talent Peaks Program” of Jiangsu Province of China (2009).

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Authors and Affiliations

  1. Department of Pharmaceutical Analysis, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210028, Jiangsu, China

    Cheng-Ying Wu

  2. Department of Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing, 210028, Jiangsu, China

    Cheng-Ying Wu

  3. The School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, Jiangsu, China

    Hong Wang, Xiu-He Fan, Wei Yue & Qi-Nan Wu

  4. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing, 210023, Jiangsu, China

    Qi-Nan Wu

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Wu, CY., Wang, H., Fan, XH. et al. Waste Euryale ferox Salisb. Leaves as a Potential Source of Anthocyanins: Extraction Optimization, Identification and Antioxidant Activities Evaluation. Waste Biomass Valor 11, 4327–4340 (2020). https://doi.org/10.1007/s12649-019-00762-2

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