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High Titer Ethanol Production from Combined Alkaline/Alkaline Hydrogen Peroxide Pretreated Bamboo at High Solid Loading

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Abstract

Enzymatic hydrolysis and fermentation at high solid loading is generally required for the production of high titer ethanol from lignocellulosic biomass at a relatively low cost. In this paper, bamboo pretreated by alkaline and alkaline hydrogen peroxide was subjected to downstream enzymatic hydrolysis and ethanol fermentation at high solid loading for the production of high titer ethanol. The effects of solid loading (5–25% w/v) and enzyme dosage (10–50 mg protein/g glucan) on sugar conversion and ethanol production during separate enzymatic hydrolysis and fermentation (SHF) and simultaneous enzymatic saccharification and co-fermentation (SSCF) processes were investigated. During the fermentation process, the pentose-hexose fermenting Saccharomyces cerevisiae LF1 strain was used. The results showed that the increase of solid loading decreased sugar conversion during enzymatic hydrolysis. At relatively high solid loadings (≥ 20% w/v), SSCF resulted in higher sugar conversion and final ethanol titer compared to SHF. With this proposed process, an ethanol concentration of 68.2 g/L, along with approximately 83.5% of glucan and 73.8% of xylan conversions, could be reached through SSCF at 20% solid loading with an enzyme dosage of only 20 mg protein/g glucan.

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Acknowledgements

The authors greatly acknowledge the support from National Natural Science Foundation of China (Grant No. 21506105). The authors are grateful to Novozymes for kindly providing the enzyme preparations.

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Authors and Affiliations

  1. Department of Biochemistry & Molecular Biology, Michigan State University, 603 Wilson Road, East Lansing, MI, 48824, USA

    Zhaoyang Yuan

  2. Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, China

    Weiqi Wei

  3. State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China

    Guodong Li

  4. Department of Chemical & Biological Engineering, University of British Columbia, 2360 East Mall, Vancouver, BC, V6T 1Z4, Canada

    Nuwan Sella Kapu

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  1. Zhaoyang Yuan
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Yuan, Z., Wei, W., Li, G. et al. High Titer Ethanol Production from Combined Alkaline/Alkaline Hydrogen Peroxide Pretreated Bamboo at High Solid Loading. Waste Biomass Valor 11, 2795–2805 (2020). https://doi.org/10.1007/s12649-019-00638-5

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