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Biochemical Properties of Carbohydrate-Active Enzymes Synthesized by Penicillium chrysogenum Using Corn Straw as Carbon Source

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Abstract

Lignocellulosic material is an alternative, renewable and cheaper source of molecules to be applied in greener industrial processes. Its utilization for this purpose requests steps of pre-treatment and hydrolysis. Filamentous fungi are receiving attention as source of plant cell wall degrading enzymes to apply in lignocellulosic biomass hydrolysis. In the present study, a strain of Penicillium chrysogenum CCDCA10756 isolated from Brazilian Cerrado soil (Savannah like biome) was evaluated as a producer of plant cell wall degrading enzymes aiming industrial application. The fungus cultivated in the presence of corn straw as sole carbon source secreted cellulases (endo-β-1,4-glucanases, cellobiohydrolases, β-glucosidases), endo-β-1,4-xylanases, and pectinases. Endo-β-1,4-xylanases and pectinases presented earlier production reaching maximum values after 3 days of growth in comparison to cellulolytic activities mostly produced after 5 days. Cellobiohydrolases and endo-β-1,4-glucanases present maximal activity in acid pH (3 and 4) and at 50 °C, whereas β-glucosidase presents maximal activity at pH 5.0 and 60 °C. Pectinases showed maximum activity in pH 8 at 50 °C. Furthermore, endo-β-1,4-glucanases and cellobiohydrolases displayed remarkable thermostability at 40 °C. Lignin-derived compounds, trans-ferulic acid, 4-hydroxybenzoic and syringaldehyde inhibited cellobiohydrolases. Pectinolytic activity, instead, was improved in the presence of p-coumaric acid, trans-ferulic acid, and syringaldehyde.

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Acknowledgements

This work was supported by research grants from the University of Brasilia – UnB, CNPq, and FAPDF. Eliane F. Noronha is recipient of Brazilian Research Council (CNPq) research scholarship. Pedro R.V Hamann, and Alonso R.P. Ticona are recipient of CAPES doctoral degree scholarship, Sadia F. Ullah is the recipient of CNPq doctoral’s degree scholarship.

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  1. Luísa de M.B.Silva and Tainah C. Gomes have equally contributed to this work.

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  1. Cell Biology Department, Enzymology Laboratory, Universidade de Brasilia, Brasilia, 70910-900, Brazil

    Luísa de M. B. Silva, Tainah C. Gomes, Sadia F. Ullah, Alonso R. P. Ticona, Pedro R. V. Hamann & Eliane F. Noronha

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Silva, L.d.M.B., Gomes, T.C., Ullah, S.F. et al. Biochemical Properties of Carbohydrate-Active Enzymes Synthesized by Penicillium chrysogenum Using Corn Straw as Carbon Source. Waste Biomass Valor 11, 2455–2466 (2020). https://doi.org/10.1007/s12649-019-00589-x

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