Tuna Condensate Waste with Molasses as a Renewable Substrate for Antifungal Compounds by Streptomyces philanthi RL-1-178 Against Aflatoxingenic B1 (AFB1) Aspergillus flavus

  • Sawai BoukaewEmail author
  • Wanida Petlamul
  • Poonsuk Prasertsan
Original Paper


This study aims to utilize tuna condensate as a renewable resource for production of antifungal compounds by Streptomyces philanthi RL-1-178 against aflatoxingenic B1 (AFB1) Aspergillus flavus. Among ten isolates of A. flavus tested, the strain PSRDC-4 was the most toxigenic strain (1432 ppb of AFB1) and aggressive to S. philanthi (85.9% inhibition). The effective dose (100% inhibition) of the culture filtrate RL-1-178 was at 10.0% (v/v) with 1 h exposure time. The antifungal compounds, identified by GC–MS analysis, were consisted of 105 components with 2,4-imidazolidinedione (31.2%) followed by acetic acid (25.27%) was the most abundant. The optimum condition for growth and production of antifungal compounds from S. philanthi RL-1-178 was as following; tuna condensate of 15,000 mg L−1 COD, the initial pH at 7.0, incubation temperature at 30 °C and supplemented with 8 g L−1 of molasses. Therefore, tuna condensate exhibited a high potential to be utilized as an alternative medium for antifungal production by S. philanthi RL-1-178.


A. flavus Antifungal activity Molasses S. philanthi Tuna condensate Waste utilization 



This research study was financially supported by the Agricultural Research Development Agency (Public Organization) (PRP5905021490) and Thailand Research Fund (RTA6080010).


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Sawai Boukaew
    • 1
    Email author
  • Wanida Petlamul
    • 1
  • Poonsuk Prasertsan
    • 2
  1. 1.College of Innovation and ManagementSongkhla Rajabhat UniversitySongkhlaThailand
  2. 2.Research and Development OfficePrince of Songkla UniversitySongkhlaThailand

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