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Black Carrot (Daucus carota ssp.) and Black Soybean (Glycine max (L.) Merr.) Anthocyanin Extract: A Remedy to Enhance Stability and Functionality of Fruit Juices by Copigmentation

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Abstract

Application of extracted black carrot anthocyanins (BCA) and black soybean anthocyanins (BSA) as colour enhancers was demonstrated by copigmenting with heat sensitive anthocyanins from plum, pomegranate, strawberry and jamun. Improved pigment stability was confirmed by thermal stability, chromacity studies and hyperchromic and bathochromic shifts. BCA (2%, 500 mg/L) caused largest hyperchromic shift in plum whereas maximum bathochromic shift (18–20 nm) was observed in strawberry. BSA (2%, 500 mg/L) caused largest hyperchromic shift in pomegranate whereas maximum bathochromic shift (22 nm) was observed in plum. Copigmented plum, jamun, strawberry and pomegranate juices copigmented with BCA achieved high half-life (t1/2) and lower rate constant (k) values. Copigmenting anthocyanins with other juice is an effective strategy to impart high thermal stability, improve colour retention and antioxidant activity.

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References

  1. Norberto, S., Sara, S., Manuela, M., Ana, F., Pintado, M.E., Conceicao, C.: Blueberry anthocyanins in health promotion: a metabolic overview. J. Funct. Foods 5(4), 1518–1528 (2013)

    Article  Google Scholar 

  2. MEYED (Fruit Juice Industry Corporation) Turkey fruit juice like products industry report (2011) http://www.meyed.org.tr

  3. Peng, H., Li, W., Li, H., Deng, Z., Zhang, B.: Extractable and non-extractable bound phenolic compositions and their antioxidant properties in seed coat and cotyledon of black soybean (Glycine max (L.) Merr.). J. Funct. Foods 32, 296–312 (2017)

    Article  Google Scholar 

  4. Wang, D., Ma, Y., Zhang, C., Zhao, X.: Thermal characterization of the anthocyanins from black soybean (Glycine max L.) exposed to thermogravimetry. LWT-Food Sci. Technol. 55(2), 645–649 (2014)

    Article  Google Scholar 

  5. Zhang, B., He, F., Zhou, P.P., Liu, Y., Dua, C.Q.: The color expression of copigmentation between malvidin-3-O-glucoside and three phenolic aldehydes in model solutions: the effects of pH and molar ratio. Food Chem. 199, 220–228 (2016)

    Article  Google Scholar 

  6. Wrolstad, R.E., Durst, R.W., Lee, J.: Tracking color and pigment changes in anthocyanin products. Trends Food Sci. Technol. 16, 423–428 (2005)

    Article  Google Scholar 

  7. Mazza, G., Miniati, E.: Anthocyanins in Fruits, Vegetables and Grains. CRC, Boca Raton (1993)

    Google Scholar 

  8. Souza, A.C.P., Gurak, P.D., Marczak, L.D.F.: Maltodextrin, pectin and soy protein isolate as carrier agents in the encapsulation of anthocyanins-rich extract from jaboticaba pomace. Food Bioprod. Process. 8, 123–129 (2016)

    Google Scholar 

  9. Khandare, V., Walia, S., Singh, M., Kaur, C.: Black carrot (Daucus carota ssp. sativus) juice: processing effects on antioxidant composition and color. Food Bioprod. Process. 89, 482–486 (2011)

    Article  Google Scholar 

  10. Singleton, V.L., Orthofer, R., Lamuela-Raventos, R.M.: Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent. Methods Enzymol. 299, 152–178 (1999)

    Article  Google Scholar 

  11. Benzie, I.F.F., Strain, J.J.: The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant” power: the FRAP assay. Anal. Biochem. 239, 70–76 (1996)

    Article  Google Scholar 

  12. Apak, R., Guclu, K., Ozyurek, M., Karademir, S.E.: Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J. Agric. Food Chem. 52, 7970–7981 (2004)

    Article  Google Scholar 

  13. Brand-Williams, W., Cuvelier, M.E., Berset, C.: Use of a free radical method to evaluate antioxidant activity. Lebensmittel-Wissenschaft und-Technologie 28, 25–30 (1995)

    Article  Google Scholar 

  14. Sanchez-Moreno, C., Larrauri, J.A., Saura-Calixto, F.: A procedure to measure the antiradical efficiency of polyphenols. J. Sci. Food Agric. 76, 270–276 (1998)

    Article  Google Scholar 

  15. Müller-Maatsch, J., Bechtold, L., Schweiggert, R.M., Carle, R.: Copigmentation of pelargonidin derivatives in strawberry and red radish model solutions by the addition of phenolic fractions from mango peels. Food Chem. 213, 625–634 (2016)

    Article  Google Scholar 

  16. Castaneda-Ovando, A., Pacheco-Hernández, M.L., Páez-Hernández, M.E., Rodríguez, J.A., Galán-Vidal, C.A.: Chemical studies of anthocyanins: a review. Food Chem. 113, 859–871 (2009)

    Article  Google Scholar 

  17. Pan, Y.Z., Guana, Y., Wei, Z.F., Penga, X., Li, T.T., Qi, X.L., Zua, Y.G., Fu, Y.J.: Flavonoid C-glycosides from pigeon pea leaves as color and anthocyanin stabilizing agent in blueberry juice. Ind. Crops Prod. 58, 142–147 (2014)

    Article  Google Scholar 

  18. Jensen, M.B., Lopez-de-Dicastillo, B.C.A., Payet, R.M., Liu, X., Konczak, I.: Influence of copigment derived from Tasmania pepper leaf on Davidson’s plum anthocyanins. J. Food Sci. 76(3), 47–53 (2011)

    Article  Google Scholar 

  19. Kopjar, M., Piližota, V., Šubarić, D., Babić, J.: Copigmentation effect of phenolic compounds on red currant juice anthocyanins during storage. Croatian J. Food Sci. Technol. 1, 16–20 (2009)

    Google Scholar 

  20. Pacheco-Palencia, L.A., Talcott, S.T.: Chemical stability of acai fruit (Euterpe oleracea Mart.) anthocyanins as influenced by naturally occurring and externally added polyphenolic cofactors in model systems. Food Chem. 118(1), 17–25 (2010)

    Article  Google Scholar 

  21. Brouillard, R.: Origin of the exceptional colour stability of the Zebrina anthocyanin. Phytochemistry 20(1), 143–145 (1981)

    Article  Google Scholar 

  22. Hsiao, H.C.: Anthocyanin color enhancement by using catechin as copigements and stability during storage [M.S. thesis]. Department of Food Science and Technology. The Ohio State University, Columbus, Ohio (2014)

  23. Markovic, J.M.D., Ignjatovic, L.M., Markovic, D.A., Baranac, J.M.: Antioxidant capabilities of some organic acids and their copigments with malvin—part II. J. Electroanal. Chem. 553, 177–182 (2003)

    Article  Google Scholar 

  24. Markovic, J.M.D., Ignjatovic, L.M., Markovic, D.A., Baranac, J.M.: Antioxidant capabilities of some organic acids and their copigments with malvin—part I. J. Electroanal. Chem. 553, 169–175 (2003)

    Article  Google Scholar 

  25. Sari, P., Wijaya, C.H., Sajuthi, D., Supratman, U.: Colour properties, stability, and free radical scavenging activity of jambolan (Syzygium cumini) fruit anthocyanins in a beverage model system: natural and copigmented anthocyanins. Food Chem. 132, 1908–1914 (2012)

    Article  Google Scholar 

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Acknowledgements

The facilities and financial support provided by the ICAR-Indian Agricultural Research Institute (IARI), Department of Science and Technology (DST), Science and Engineering Research Board (SERB), Confederation of Indian Industry (CII) and Prathista Industries Limited under Prime Minister Fellowship Scheme to undertake the research work are highly acknowledged. The author was a recipient of Prime Minister’s Fellowship Scheme for Doctoral Research, a public–private partnership between Science and Engineering Research Board (SERB), Department of Science and Technology, Government of India and Confederation of Indian Industry (CII). The author’s host institute for research was ICAR-Indian Agricultural Research Institute and the partner company was Prathista Industries Ltd.

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Correspondence to Manoj Kumar.

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Kumar, M., Dahuja, A., Sachdev, A. et al. Black Carrot (Daucus carota ssp.) and Black Soybean (Glycine max (L.) Merr.) Anthocyanin Extract: A Remedy to Enhance Stability and Functionality of Fruit Juices by Copigmentation. Waste Biomass Valor 11, 99–108 (2020). https://doi.org/10.1007/s12649-018-0450-3

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