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Efficient Biocatalytic Conversion of Stranded Green Macroalgal Biomass Using a Specific Cellulases-Based Cocktail

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Abstract

A specific macroalgal biomass decomposing fungus SL1 newly isolated and identified as Aspergillus fumigatus was found to be an efficient cellulases producer. This strain when cultivated on the whole green macroalgae as sole carbon source permitted the production of specific cellulases (specific activities of 30 and 33 U/mg of proteins for endoglucanase and β-glucosidase, respectively) different from commercial ones as shown by zymography. The application of the produced cellulases-based enzymatic cocktail for the saccharification of alkali pretreated Ulva sp. biomass yielded of 58%. This saccharification rate was optimized using response surface methodology (RSM). An increase of 36% in saccharification yield was obtained under optimized conditions (13 U CMCase, 4% substrate and 135 rpm agitation) which agreed with model predictions. The biocatalytic conversion using specific fungal cellulases may be a promising approach for the biodegradation of stranded macroalgae and its valorization mostly for bioethanol production.

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Acknowledgements

The authors gratefully acknowledge the Tunisian Ministry of Higher Education and Scientific Research - University of Carthage (Tunisia) for financial support (project LR11ES24) and for concession of a research grant for Nesrine Ben Yahmed’s Ph.D. We express our gratitude to Maher Chelly who helped to the determination of the preliminary range of the saccharification variables.

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  1. Laboratoire LIP-MB INSAT, LR11ES26, Université de Carthage, INSAT-BP 676, Centre urbain nord, 1080, Carthage Cedex, Tunisia

    Nesrine Ben Yahmed, Nadia Berrejeb, Mohamed Amine Jmel, Souhir Jazzar, M. Nejib Marzouki & Issam Smaali

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  1. Nesrine Ben Yahmed
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  2. Nadia Berrejeb
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  3. Mohamed Amine Jmel
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Ben Yahmed, N., Berrejeb, N., Jmel, M.A. et al. Efficient Biocatalytic Conversion of Stranded Green Macroalgal Biomass Using a Specific Cellulases-Based Cocktail. Waste Biomass Valor 11, 211–222 (2020). https://doi.org/10.1007/s12649-018-0397-4

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