Synthesis of Silver Nanoparticles Using Extracts from Yerba Mate (Ilex paraguariensis) Wastes

  • Romina A. ArrecheEmail author
  • Gabriela Montes de Oca-Vásquez
  • Jose R. Vega-Baudrit
  • Patricia G. Vázquez
Original Paper


Synthesis of metallic nanoparticles by an eco-friendly and sustainable process is an important target to be developed in nanotechnology area. In the present work, two different commercial brands of yerba mate from Argentina and their wastes (PYM and TYM samples) were used for the preparation of aqueous extracts, in order to synthesize silver nanoparticles at room temperature (25 °C). The silver nanoparticles obtained were spherical, hexagonal and, triangular in shape with the average particle size of 50 nm and, shows a surface plasmon peak around 460 nm. The antimicrobial activity of the silver nanoparticles obtained with the extracts from yerba mate wastes was evaluated against E. coli and S. aureus. The minimum inhibitory concentrations required for E. coli were 7.66 and 17.66 µg ml−1 using the treatment T2YE and P2YE, respectively and, for S. aureus were 23.25 and 50.60 µg ml−1 for the treatment T2YE and P2YE, respectively. The study suggests that polyphenols present in I. paraguariensis leaf extract act as reducing agent and stabilizer of the nanoparticles.

Graphical Abstract


Yerba mate Wastes Silver nanoparticles Eco-friendly synthesis Transmission electronic microscopy Antibacterial activity 



The authors would like to express their thanks for the financial support provided by Laboratorio Nacional de Nanotecnología (LANOTEC), Centro Nacional de Alta Tecnología (CeNAT-CONARE), POLIUNA-UNIVERSIDAD NACIONAL de Costa Rica, National Scientific and Technical Research Council (CONICET) and National University of La Plata (UNLP). They also thank to Reynaldo Pereira for TEM micrographs, Universidad Nacional for the Dynamic light scattering (DLS) and Zeta potential analysis, and Yendry Corrales Ureña for AFM images.


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.CINDECA – Centro de Investigación y Desarrollo en Ciencias Aplicadas, ¨Dr. Jorge J. Ronco¨, CONICET – CIC – UNLPLa PlataArgentina
  2. 2.LANOTEC – National Laboratory of Nanotechnology, Centro Nacional de Alta Tecnología (CeNAT – CONARE)San JoseCosta Rica

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