Optimization of Process Parameters for Efficient Bioconversion of Thermo-chemo Pretreated Manihot esculenta Crantz YTP1 Stem to Ethanol

  • P. Selvakumar
  • S. Kavitha
  • P. Sivashanmugam
Original Paper


The production of value-added products from lignocellulosic waste has been of great interest for economic as well as environmental concerns. Also, lignocellulosic waste constitutes an attractive renewable feedstock for bioethanol production due to their abundance, availability and low cost. Ethanol production from Manihot esculenta Crantz YTP1 stem was investigated through consolidated bioprocessing (CBP) using Cellulomonas fimi MTCC 24 and Zymomonas mobilis MTCC 92. CBP integrates enzyme production, saccharification (of pre-treated biomass) and fermentation in a single reactor. Screening and optimization of pre-treatment with different acids namely CH3COOH, HNO3, and combined acids (CH3COOH + HNO3) in different dosages, temperature and hydrolysis time, were evaluated for efficient de-lignification of 85.0 ± 3.2% and maximum cellulose release of 87.45 ± 2.2% of biomass. The pre-treatment data obtained were statistically validated using one-way ANOVA. Process parameters such as pH, temperature, agitation and time on the co-production of cellulase and ethanol were statistically optimized by response surface methodology (RSM) and artificial neural network (ANN). The maximum cellulase of 11.63 ± 1.23 IU/mL and ethanol of 9.39 ± 0.33 g/L were obtained in pre-treated biomass. This study demonstrates that M. esculenta Crantz YTP1 stem is an alternative feedstock, and these optimized conditions could be successfully used for commercial production of ethanol using C. fimi MTCC 24 and Z. mobilis MTCC 92 through CBP.


Manihot esculenta Crantz YTP1 Biomass pretreatment Kinetic studies Cellulase Ethanol production Response surface methodology Artificial neural network 


Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

12649_2018_244_MOESM1_ESM.docx (1.3 mb)
Supplementary material 1 (DOCX 1294 KB)


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© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Chemical EngineeringNational Institute of TechnologyTiruchirappalliIndia

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