Abstract
The goal of this study was to isolate and characterize a new bacterium capable of tolerating high concentrations of toxic organic solvents. The production of several extracellular secondary metabolites by Pseudomonas aeruginosa IBBPo16 cells was investigated using a combination of cultural, biochemical and molecular methods. A gram-negative bacterium, P. aeruginosa strain IBBPo16 (GenBank KT315654) was isolated from Poeni oily sludge by enrichment cultures method. Based on 16S rRNA gene sequence, isolated bacterium exhibited 100% similarity with other P. aeruginosa strains from nucleotide database. P. aeruginosa IBBPo16 was able to tolerate 100% cyclohexane, n-hexane, n-decane, styrene, 40% ethylbenzene, and 5% toluene. Toluene and ethylbenzene were more toxic for this bacterium, as compared with cyclohexane, n-hexane, n-decane, and styrene. In the genomic DNA extracted from P. aeruginosa IBBPo16 cells alkane hydroxylase (alkB1), xylene monooxygenase (xylM), naphthalene dioxygenase (ndoM), hydrophobic/amphiphilic efflux 1 (HAE1), and rhamnosyltransferase 1 (rhlAB) genes were detected. P. aeruginosa IBBPo16 produced some extracellular secondary metabolites, such as rhamnolipid surfactant, pyocyanin, pyorubin and pyoverdin pigments, which are well recognized for their numerous potential applications.
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The study was funded by Project No. RO1567-IBB05/2017 from the Institute of Biology Bucharest of Romanian Academy. The author is grateful to Ana Dinu for technical support.
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Stancu, M.M. Production of Some Extracellular Metabolites by a Solvent-Tolerant Pseudomonas aeruginosa Strain. Waste Biomass Valor 9, 1747–1755 (2018). https://doi.org/10.1007/s12649-017-9967-0
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DOI: https://doi.org/10.1007/s12649-017-9967-0