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Harvesting of Botryococcus sp. Biomass from Greywater by Natural Coagulants

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Abstract

Harvesting of Botryococcus sp. from greywater by Moringa oleifera and Strychnos potatorum seed flours was optimized by response surface methodology. Three factors including dosage (10–60 mg L−1), settling time (30–120 min), and pH (6–9) were investigated. Optimal operating parameters were obtained with dosage of 35 mg L−1, pH of 7.5, and settling time of 75 min, which demonstrated predicted and experimental recovery of 86.80 and 85.60% for M. oleifera, and 41.33 and 38.30% for S. potatorum, with R2 coefficient of 0.947 and 0.953, respectively. The interaction between the factors was significant (p < 0.05) at the optimal operating parameters. Scanning electron microscope (SEM) observed that the surface appearance of M. oleifera seed flour was smoother compared to S. potatorum, which reflects the higher potential of M. oleifera seed flour to adhere to microalgae cells. Natural coagulants have the potential as an alternative technique for harvesting microalgae from greywater.

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Acknowledgements

The authors are grateful for the financial support provided by the Fundamental Research Grant Scheme (FRGS) Vot 1453 from the Ministry of Education, Malaysia. We also thankful to get a support from ORICC UTHM through research grants STG Vot U659 and IGSP Vot U682.

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Correspondence to Radin Maya Saphira Radin Mohamed or Adel A. Al-Gheethi.

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Hauwa, A., Mohamed, R.M.S.R., Al-Gheethi, A.A. et al. Harvesting of Botryococcus sp. Biomass from Greywater by Natural Coagulants. Waste Biomass Valor 9, 1841–1853 (2018). https://doi.org/10.1007/s12649-017-9958-1

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  • DOI: https://doi.org/10.1007/s12649-017-9958-1

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