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Characterization and Strain Improvement of Aerobic Denitrifying EPS Producing Bacterium Bacillus cereus PB88 for Shrimp Water Quality Management

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Abstract

A bacterial strain PB88 was isolated from intensive shrimp culture pond to study the denitrification process. On the basis of 16S rDNA analysis, strain PB88 was identified as Bacillus cereus PB88. It has the potential to remove 82.33 ± 3.24% \({\text{NO}}_{2}^{ - } {-} {\text{N}}\)in synthetic medium. The optimum pH, temperature and dissolved oxygen for the highest denitrification process of the PB88 were 8.0, 30 °C and 5.21 mg/l (150 rpm) respectively. PB88 harbour the genetic sequence of nitrite reductase (nirS) enzyme which is essential to complete aerobic denitrification process. One remarkable finding is that the experimental organism produced exopolysaccharide (EPS) during the denitrification process and EPS has the antibacterial property against shrimp pathogen Vibrio harveyi MTCC 7954 (inhibition zone of 5.21 mm) and Vibrio vulnificus MTCC 1145 (inhibition zone of 7.11 mm). Removal of \({\text{NO}}_{2}^{ - } {-} {\text{N}}\) in open base shrimp wastewater system were recorded as 98.51% by B. cereus PB88 and average shrimp body weight gained in treated system as 6 ± 0.54 to 8 ± 0.74 g within 7 days. Overall result indicated that B. cereus PB88 has the immense potential for the application in commercial shrimp culture as denitrifying agent.

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Acknowledgements

The authors are thankful to Mr. D. K. Chopra group CEO of Biostadt India Limited, Mumbai, India for his kind support and Mr. Pravas Singh Assistant professor, Department of Computer Science, Vidyasagar University for development of equation.

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Authors and Affiliations

  1. Department of Microbiology, Vidyasagar University, Midnapore, West Bengal, 721102, India

    Prasenjit Barman, Tanmay Paul, Keshab Chandra Mondal & Pradeep Kumar Das Mohapatra

  2. Biostadt India Limited, Poonam Chambers ‘A’ Wing, 6th Floor, Dr. A.B. Road, Worli, Mumbai, Maharashtra, 400018, India

    Partha Bandyopadhyay

  3. Department of Detergent and Chemical Technologies, Research and Development Center, Hayat Kimya, 41275, Gölcük, Kocaeli, Turkey

    Ahmet Kati

  4. Chemical Biology Labortory, Department of Sericulture, Raiganj University, Raiganj, Uttar Dinajpur, West Bengal, 733134, India

    Amit Kumar Mandal

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  1. Prasenjit Barman
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Correspondence to Pradeep Kumar Das Mohapatra.

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Barman, P., Bandyopadhyay, P., Kati, A. et al. Characterization and Strain Improvement of Aerobic Denitrifying EPS Producing Bacterium Bacillus cereus PB88 for Shrimp Water Quality Management. Waste Biomass Valor 9, 1319–1330 (2018). https://doi.org/10.1007/s12649-017-9912-2

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