Skip to main content

Advertisement

SpringerLink
Log in

Obtaining and Characterization of Mesophilic Bacterial Consortia from Tropical Sludges Applied on Biohydrogen Production

  • Original Paper
  • Published:
Waste and Biomass Valorization Aims and scope Submit manuscript

Abstract

The biohydrogen production from different tropical sludges of biologic treatment plants was investigated in anaerobic batch reactors fed with sucrose in concentrations similar to food wastewaters. The tropical sludges tested were: I—granular sludge from a upflow anaerobic sludge blanket (UASB) reactor treating brewery wastewater; II—facultative anaerobic sludge from sedimentation pond treating domestic sewage; III—granular sludge from UASB reactor treating domestic sewage. The anaerobic batch reactors fed with 2 g L−1 of sucrose at pH 5.5 and 37 °C showed higher production of H2 than all tropical sludges tested. The maximum yield was 2.0 mol H2 mol of sucrose−1. The intermediary products during fermentation were acetic and butyric acids. Therefore, the bioproduction probably followed both the acetic acid and butyric acid route. A wide diversity of hydrogen producing bacteria identified as Clostridium sp., Bacillus megaterium, Staphylococcus sp., Bacillus subtilis and Lactobacillus sp. was observed by phylogenetic analysis. Tropical sludges from biologic treatment plants can be applied on biohydrogen production.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Song, X., Dai, Y., Fan, Q.L., Li, X.H., Fan, Y.T., Hou, H.W.: Effects of pretreatment method of natural bacteria source on microbial community and bio-hydrogen production by dark fermentation. Int. J. Hydrog. Energy 37, 5631–5636 (2012)

    Article  Google Scholar 

  2. Mohanapriya, C., Krishnaven, M.: Gas chromatography analysis for hydrogen production from Bacillus subtilis MKMP. Int. J. Chem. Anal. Sci. 4, 182–184 (2013)

    Article  Google Scholar 

  3. Maintinguer, S.I., Fernandes, B.S., Saavedra, N., Duarte, I., Adorno, M.A.T., Varesche, M.B.A.: Fermentative hydrogen production with xylose by Clostridium and Klebsiella species in anaerobic batch reactors. Int. J. Hydrog. Energy 36, 13508–13517 (2011)

    Article  Google Scholar 

  4. Lorencini, P., Siqueira, M.R., Maniglia, B.C., Tapia, D.R., Maintinguer, S.I., Reginatto, V.: Biohydrogen production from liquid and solid fractions of sugarcane bagasse after optimized pretreatment with hydrochloric acid. Waste Biomass Valor. 7(5), 1017–1029 (2016)

    Article  Google Scholar 

  5. Martinelli, L.A., Filoso, S.: Expansion of sugarcane ethanol production in brazil: environmental and social challenges. Ecol. Appl. 18(4), 885–898 (2008)

    Article  Google Scholar 

  6. Rudorff, B.F.T., Aguiar, D.A., Silva, W.F., Sugawara, L.M., Adami, M., Moreira, M.A.: Studies on the rapid expansion of sugarcane for ethanol production in São Paulo State (Brazil) using Landasat Data. Remote Sens. 2, 1057–1076 (2010)

    Article  Google Scholar 

  7. Nonino, C.A. Maiores usinas de álcool são da região de Ribeirão Preto. COPERCANA. (2009)http://www.copercana.com.br/index.php?xvar=ver-ultimas&id=3354

  8. United States Department of Agriculture. National Agricultural Statistics Service, 2015. Florida Citrus Statistics 2013–2014, USDA-NASS. https://www.nass.usda.gov/Statistics_by_State/Florida/Publications/Citrus/fcs/2013-14/fcs1314.pdf. Accessed 28 Apr 2016

  9. Maintinguer, S.I., Fernandes, B.S., Saavedra, N., Duarte, I., Adorno, M.A.T., Varesche, M.B.A.: Fermentative hydrogen production by microbial consortium. Int. J. Hydrog. Energy 33, 4309–4317 (2008)

    Article  Google Scholar 

  10. Lin, C.Y., Cheng, C.H.: Fermentative hydrogen production from xylose using anaerobic mixed microflora. Int. J. Hydrog. Energy 31, 832–840 (2006)

    Article  Google Scholar 

  11. Das, D., Veziroglu, T.N.: Hydrogen production by biological processes: a survey of literature. Int. J. Hydrog. Energy 26, 13–28 (2001)

    Article  Google Scholar 

  12. Levin, B., Pitt, L., Love, M.: Biohydrogen production: prospects and limitations to practical application. Int. J. Hydrog. Energy 29, 173–185 (2004)

    Article  Google Scholar 

  13. Oh, S.E., Ginkel, S.V., Logan, B.E.: The relative effectiveness of pH control and heat treatment for enhancing biohydrogen gas production. Environ. Sci. Technol. 37, 5186–5190 (2003)

    Article  Google Scholar 

  14. Del Nery, V.: PhD Thesis. Use of anaerobic sludge immobilized in gel in the study of the departure of reactors with upflow sludge blanket. Engineering School of São Carlos, São Paulo University, São Carlos, São Paulo State, Brazil (1987)

  15. Rossi, D.M., Costa, J.B., Souza, E.A., Peralba, M.C.R., Samios, D., Ayub, M.A.Z.: Comparison of different pretreatment methods for hydrogen Production using environmental microbial on residual glycerol from biodiesel. Int. J. Hydrog. Energy 36, 4814–4819 (2011)

    Article  Google Scholar 

  16. Widdel, F., Pfennig, N.:. Genus Desulfobacter. In Bergey’s Manual of Systematic Bacteriology, p. 1599 (1984)

  17. Dhole, V., Kadam, V.: Advantages of the TOGA-Transformer Oil Gas Analyzer Involving Headspace-GC Analysis and a DGA System. Application note 10348. Thermo Fisher Scientific, Nasik (2012)

    Google Scholar 

  18. Adorno, M.A.T., Hirasawa, J.S., Varesche, M.B.A.: Development and validation of two methods to quantify volatile acids (C2-C6) by GC/FID: headspace (automatic and manual) and liquid-liquid extraction (LLE). Am. J. Anal. Chem. 5, 406–414 (2014)

    Article  Google Scholar 

  19. Dubois, M., Gilles, K.A.K.A., Hamilton, J.K.J., Rebers, P.A., Smith, F.: Colorimetric method fordetermination of sugars and related substances. Anal. Chem. 28, 350–356 (1956)

    Article  Google Scholar 

  20. Herbert, D., Philipps, O.S., Strang, R.E.: Carbohydrate analysis. Methods Enzymol. 5B, 265–277 (1971)

    Google Scholar 

  21. American Public Health Association/American Water Works Association/Water Environment Federation (APHA/AWWA/WEF) (2005) Standard Methods for the Examination of Water and Wastewater, 21st edn. American Public Health Association, Washington DC

    Google Scholar 

  22. Griffiths, R.I., Whiteley, A.S., O’Donnell, A.G.: Rapid method for coextration of DNA and RNA from natural environments for analysis of ribosomal DNA and rRNA-based microbial community composition. Appl. Environ. Microbiol. 66, 5488–5491 (2000)

    Article  Google Scholar 

  23. Nubel, U., Engelen, B., Felske, A., Snaidr, J., Wieshuber, A., Amann, R.I., Ludwig, W., Backhaus, H.: Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacilluspolymyxa detected by temperature gradient gel electrophoresis. J. Bacteriol. 178(19), 5636–5643 (1996)

    Article  Google Scholar 

  24. Maintinguer, S.I., Sakamoto, I.K., Adorno, M.A.T., Varesche, M.B.A.: Bacterial diversity from environmental sample applied to bio-hydrogen production. Int. J. Hydrog. Energy 40, 3180–3190 (2015)

    Article  Google Scholar 

  25. http://www.macrogen.com

  26. Abreu, A.A., Alves, J.I., Pereira, M.A., Sousa, D.Z., Alves, M.M.: Strategies to suppress hydrogen-consuming microorganisms affect macro and micro scale structure and microbiology of granular sludge. Biotechnol. Bioeng. 108, 1766–1775 (2011)

    Article  Google Scholar 

  27. Lay, J.Y., Li, Y.Y., Noike, T.: Developments of bacterial population and methanogenic activity in a laboratory-scale landfill bioreactor. Water Res. 32, 3673–3679 (1998)

    Article  Google Scholar 

  28. Wang, R., Zong, W., Qian, C., Wei, Y., Yu, R., Zhou, Z.: Isolation of Clostridium perfringens strain W11 and optimization of its biohydrogen production by genetic modification. Int. J. Hydrog. Energy 36, 12159–12167 (2011)

    Article  Google Scholar 

  29. Subudhi, S., Nayak, T., RamKumar, N., Vijayananth, P., Lal, B.: Impact of regulated pH on proto scale hydrogen production from xylose by an alcaline tolerant novel bacterial strain, Enterobacter cloacae DT-1. Int. J. Hydrog. Energy 38, 2728–2737 (2013)

    Article  Google Scholar 

  30. Liu, H., Zhang, T., Fang, H.H.P.: Thermophilic H2 productionfrom a cellulose-containing wastewater. Biotechnol. Lett. 25, 365–369 (2003)

    Article  Google Scholar 

  31. Jame, R., Vilímová, V., Lakatos, B., Vaereek, L.: The hydrogen production by anaerobic grown on glucose and glycerol. Acta Chim. Slov. 4, 145–157 (2011)

    Google Scholar 

  32. Khanal, S.K., Chen, W.H., Li, L., Sung, S.: Biological hydrogen production: effects and intermediate products. Int. J. Hydrog. Energy 29, 1123–1131 (2004)

    Google Scholar 

  33. Sá, L.R.V., Cammarota, M.C., Oliveira, T.C., Oliveira, E.M.M., Matos, A., Ferreira-Leitão, V.S.: Pentoses hexoses and glycerin as substrates for biohydrogen production: an approach for Brazilian biofuel integration. Int. J. Hydrog. Energy 38, 2986–2997 (2013)

    Article  Google Scholar 

  34. Castelló, E., Santos, C.G., Iglesias, T., Paolino, G., Wenzel, J., Borzacconi, L., Etchebehere, C.: Feasibility of biohydrogen production from cheese whey using a UASB reactor: links between microbial community and reactor performance. Int. J. Hydrog. Energy 34, 5674–5682 (2009)

    Article  Google Scholar 

  35. Kawagoshi, Y., Hino, N., Fujimoto, A., Nakao, M., Fujita, Y., Sugimur, S., Furukawa, K.: Effect of seed sludge conditioning on hydrogen fermentation and pH effect on bacterial community relevant to hydrogen production. J. Biosci. Bioeng. 100,(5), 524–530 (2005)

    Article  Google Scholar 

  36. Ratti, R.P., Botta, L.S., Sakamoto, I.K., Silva, E.L., Varesche, M.B.A.: Production of H2 from cellulose by rumen microorganisms:effects of inocula pre-treatment and enzymatic hydrolysis. Biotechnol. Lett. 36, 537–546 (2014)

    Article  Google Scholar 

  37. Zhao, L., Cao, G., Wang, A., Guo, W., Liu, B., Ren, H., Ren, N., Ma, F.: Enhanced bio-hydrogen production by immobilized Clostridium sp. T2 on a new biological carrier. Int. J. Hydrog. Energy 37, 162–166 (2012)

    Article  Google Scholar 

  38. Hoffman, M.B., Lukoynov, D., Yang, Z.Y., Dean, D.R., Seefeldt, L.: Mehcanism of nitrogen fixation by nitrogenase: the next stage. Chem. Rev. 114, 4041–4062 (2014)

    Article  Google Scholar 

  39. Shida, G.M., Sader, L.T., Amorim, E.L.C., Sakamoto, I.K., Maintinguer, S.I., Saavedra, N.K., Varesche, M.B.A., Silva, E.L.: Performance and composition of bacterial communities in anaerobic fluidized bed reactors for hydrogen production: effects of organic loading rate and alkalinity. Int. J. Hydrog. Energy 37, 16925–16934 (2012)

    Article  Google Scholar 

  40. Terrazas-Hoyo, H., Portugal, M.E., Sánches, S.E., Ortiz-Hernandez, M.L.: Evaluation of the potential hydrogen production by diazotrophic Burkholderia species. Int. J. Hydrog. Energy 39, 3142–3151 (2014)

    Article  Google Scholar 

  41. Etchebehere, C., Castelló, E., Wnzel, J., Anzola-Rojas, M.P., Borzacconi, L., Buitrón, G., Cabrol, L., Carminato, V.M., Carrillo-Reyes, J., Cisneros-Pérez, C., Fuentes, L., Moreno-Andrade, I., Razo-Flores, E., Filippi, G.R., Tapia-Venegas, E., Toledo-Alarcón, J., Zaiat, M.: Microbial communities from 20 different hydrogen-producing reactors studied by 454 pyrosequencing. Appl. Microbiol. Biotechnol. 100(Issue 7), 3371–3384 (2016)

    Article  Google Scholar 

  42. Vary, P.S., Biedendieck, R., Fuerch, T., Meinhardt, F., Rohde, M., Deckwer, W.D., Jahn, D.: Bacillus megaterium—from simple soil bacterium to industrial protein production host. Appl. Microbiol. Biotechnol. 76, 957–967 (1998)

    Article  Google Scholar 

Download references

Acknowledgements

The authors acknowledge Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP—Process No. 2012/01318-01) and Fundação para o Desenvolvimento da Unesp (Fundunesp – Process 1001/2003) for financial support, and Cempeqc (Center for Monitoring and Researching the Quality of Fuels, Biofuels, Crude Oil, and Derivatives) for the laboratory facilities.

Author information

Authors and Affiliations

  1. CEMPEQC - Center for Monitoring and Research of the Quality of Fuels, Biofuels, Crude Oil, and Derivatives, Institute of Chemistry, Unesp, Araraquara, SP, 14801-970, Brazil

    Renan Pachiega, Rafael Rodrigues Hatanaka & José Eduardo de Oliveira

  2. IPBEN – Bioenergy Research Institute, Unesp, Rio Claro, SP, 13500-230, Brazil

    Renan Pachiega & Sandra Imaculada Maintinguer

  3. Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo, São Carlos, SP, 13563-120, Brazil

    Isabel Kimiko Sakamoto & Maria Bernadete Varesche

  4. UNIARA – University of Araraquara, Araraquara, SP, 14801-320, Brazil

    Sandra Imaculada Maintinguer

Authors
  1. Renan Pachiega
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Isabel Kimiko Sakamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Maria Bernadete Varesche
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rafael Rodrigues Hatanaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. José Eduardo de Oliveira
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Sandra Imaculada Maintinguer
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Renan Pachiega.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Pachiega, R., Sakamoto, I.K., Varesche, M.B. et al. Obtaining and Characterization of Mesophilic Bacterial Consortia from Tropical Sludges Applied on Biohydrogen Production. Waste Biomass Valor 10, 1493–1502 (2019). https://doi.org/10.1007/s12649-017-0185-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12649-017-0185-6

Keywords

Search

Navigation