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Valorization of Grapefruit By-Products as Solid Support for Solid-State Fermentation to Produce Antioxidant Bioactive Extracts

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Abstract

Solid-state fermentation is a microbial process carried out mostly on the surface of solid materials. Grapefruit by-products are solids that can be used as a substrate-support matrix for fungal growth in solid fermentation. On the other hand, with the fermentation, the valorization of grapefruit by-products can be done because of recovery of secondary metabolites such as antioxidants. Fermentations were done using Raimbault columns as bioreactors. Two mixture levels (50 and 70% moisture content) were evaluated. Fungal growth was analysed online by CO2 quantification for fermentation of grapefruit by-products with Aspergillus niger GH1. Ethanolic extracts were used for antioxidant analyses (FRAP, LOI and DPPH·radical-scavenging). A. niger GH1 showed better growth on grapefruit by-products at 70% moisture content. Recovery of antioxidant compounds and solids reduction (50% of solids loss) was also higher at 70% moisture content. These results suggest that solid-state fermentation has great potential for valorization of grapefruit by-products as a support and a carbon source to produce antioxidants.

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Acknowledgements

RLC thanks the Mexican Council for Science and Technology (CONACYT) Mexico for the financial support during his MSc studies.

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Correspondence to Cristóbal N. Aguilar.

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Larios-Cruz, R., Buenrostro-Figueroa, J., Prado-Barragán, A. et al. Valorization of Grapefruit By-Products as Solid Support for Solid-State Fermentation to Produce Antioxidant Bioactive Extracts. Waste Biomass Valor 10, 763–769 (2019). https://doi.org/10.1007/s12649-017-0156-y

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Keywords

  • Aspergillus niger GH1
  • CO2 production
  • Antioxidant extraction
  • Packed-bed bioreactor