Waste and Biomass Valorization

, Volume 9, Issue 3, pp 369–377 | Cite as

Development of a Novel Micro-Aerobic Cultivation Strategy for High Potential CotA Laccase Production

  • Nadia A. Samak
  • Jianhua Hu
  • Kefeng Wang
  • Chen Guo
  • Chunzhao Liu
Original Paper


Recently, bacterial laccases has drawn researchers’ interest due to their ability to overcome high pH and salt concentration conditions compared to fungal laccases. Here we report a novel micro-aerobic cultivation strategy for enhancing CotA laccase expression and study its application for dye decolorization. Micro-aerobic cultivation of Escherichia coli BL21 (DE3) strain carrying pT7-FLAG-MAT-TAG-1-CotA had significantly enhanced CotA laccase activity up to 13903 U/L. The most unique findings of this investigation are that micro-aerobic cultivation strategy enhanced the reactive oxygen species production which consequently led to the over expression of CotA laccase gene. Malachite green, Crystal violet, Congo red and Bromophenol blue were efficiently decolorized by using purified CotA laccase without presence of any mediators at pH 6 and 9. These results provide a great platform for the dynamic production and application of bacterial laccase in industry.


Bacillus subtilis Bacterial laccase CotA IPTG Micro-aerobic condition ROS 



The funding of this study was supported by the National Key Technology Research and Development Program of China (No. 2015BAK45B01), the National Natural Science Foundation of China (No. 21476242), and the National Basic Research Program (973 Program) of China (No. 2013CB733600). We would like to acknowledge the Chinese Government for the financial support of the Ph. D scholarship awarded to Nadia A. Samak.


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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Nadia A. Samak
    • 1
    • 2
  • Jianhua Hu
    • 1
    • 2
  • Kefeng Wang
    • 1
    • 2
  • Chen Guo
    • 1
  • Chunzhao Liu
    • 1
  1. 1.State Key Laboratory of Biochemical Engineering & Key Laboratory of Green Process and Engineering, Institute of Process EngineeringChinese Academy of SciencesBeijingPeople’s Republic of China
  2. 2.University of Chinese Academy of SciencesBeijingPeople’s Republic of China

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