Waste and Biomass Valorization

, Volume 8, Issue 4, pp 1343–1350 | Cite as

Evaluation of Waste of the Cheese Industry for the Production of Aroma of Roses (Phenylethyl Alcohol)

  • L. Conde-Báez
  • J. Castro-Rosas
  • J. R. Villagómez-Ibarra
  • J. B. Páez-Lerma
  • C. Gómez-AldapaEmail author
Original Paper


The whey is a by-product obtained during the manufacture of cheese. It includes three types of whey; sweet (SW), acid (AW) and curd (CW). These residues are rich in lactose and proteins. Practically, there are no studies on the use of the three types of whey, as a substrate of micro-organisms for the production of phenylethyl alcohol (PEA). We assessed the production of PEA by Kluyveromyces marxianus using SW, AW and CW as substrate. Whey pH was adjusted to 4.8, pasteurized at 63 °C/30 min and inoculated with K. marxianus (1 × 106 CFU mL−1). The inoculated whey was embedded in agitation (180 rpm) at 30 °C for 96 h. The identification and quantification of PEA was performed by gas chromatography. In addition, it was determined the percentage of (α–β) lactose by molecular structure with nuclear magnetic resonance. K. marxianus was capable of producing PEA in the three types of whey (SW, AW and CV) in maximum concentrations of 0.96, 0.70, and 0.47 g L−1, respectively. For SW (maximum concentration of PEA), it was found a concentration of β-lactose 82.35 %. Produced by the cheese industry, whey could be used as an alternative for the production of PEA by K.marxianus.


Phenylethyl alcohol Dairy waste NMR Lactose Kluyveromyces marxianus 



Thanks to the National Council for Science and Technology (CONACYT) for the scholarship given (263643) and the producer of dairy PROUNILAC for the support access to biological samples.


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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.Área Académica de Química (AAQ), Instituto de Ciencias Básicas e Ingeniería (ICBI), Ciudad del ConocimientoUniversidad Autónoma del Estado de Hidalgo (UAEH)Mineral de la ReformaMexico
  2. 2.Departamento de Ingeniería Química-BioquímicaInstituto Tecnológico de DurangoDurangoMexico

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