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Sophorolipids Production by Candida bombicola ATCC 22214 and Its Potential Application in Soil Bioremediation

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Abstract

The sophorolipids are biosurfactants composed by a disaccharide sophorose linked by a hydroxyl group of a fatty acid. Due to their surface-active properties, sophorolipids have a great potential for using in different industrial sectors. They are synthesized by various strains of the genus Candida in the presence of hydrophilic and hydrophobic sources. In this study, it was evaluated the production of sophorolipids by Candida bombicola ATCC 22214 using sugar cane molasses, sugar cane juice, sucrose or glucose as hydrophilic source and chicken fat or sunflower oil as hydrophobic source. The sophorolipids were characterized and applied in bioremediation of soils contaminated with lubricating oil. The production of sophorolipids was 39.81 g L−1 in the optimized condition of 75 g L−1 of chicken fat, 77.5 g L−1 of glucose, 2.5 g L−1 of yeast extract and without urea. The sophorolipids produced present the diacetylated lactonic form, exhibiting the surface tension properties of 35 mN/m and of CMC 65 mg L−1. The sophorolipids showed great emulsification index in lubricant oil, toluene and n-heptane, suggesting the potential application in bioremediation of lubricating-oil-contaminated soils.

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Acknowledgments

The authors like to thank the financial support provided by CAPES(PNPD), FINEP, Fundação Araucária—Brazil, and the Laboratório de Espectroscopia (ESPEC) e Laboratório de Análise de Materiais e Moléculas (LAMM) for the analyses.

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Authors and Affiliations

  1. Departamento de Bioquímica e Biotecnologia, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445 Km 380, Campus Universitário, Cx. Postal 10.011, Londrina, Paraná, CEP 86.057-970, Brazil

    Tamires Minucelli, Renato Márcio Ribeiro-Viana, Cristiani Baldo & Maria Antonia Pedrine Colabone Celligoi

  2. Departamento de Química, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445 Km 380, Campus Universitário, Cx. Postal 10.011, Londrina, Paraná, CEP 86.057-970, Brazil

    Dionísio Borsato

  3. Departamento de Microbiologia, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445 Km 380, Campus Universitário, Cx. Postal 10.011, Londrina, Paraná, CEP 86.057-970, Brazil

    Galdino Andrade & Martha Viviana Torres Cely

  4. Departamento Tecnologia de Alimentos, Universidade Tecnológica Federal do Paraná, Avenida dos Pioneiros, 3131, Londrina, Paraná, CEP 86036-370, Brazil

    Marcos Roberto de Oliveira

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  1. Tamires Minucelli
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  2. Renato Márcio Ribeiro-Viana
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  8. Maria Antonia Pedrine Colabone Celligoi
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Correspondence to Maria Antonia Pedrine Colabone Celligoi.

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Minucelli, T., Ribeiro-Viana, R.M., Borsato, D. et al. Sophorolipids Production by Candida bombicola ATCC 22214 and Its Potential Application in Soil Bioremediation. Waste Biomass Valor 8, 743–753 (2017). https://doi.org/10.1007/s12649-016-9592-3

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