Abstract
Polyethylene glycol (PEG) 2000/phosphate salt based aqueous two-phase systems (ATPS) were used to recover phenolic compounds with antioxidant activity from wastes of eucalyptus wood veneers. Combinations of two phosphate salts (K2HPO4 or mixtures of K2HPO4 and KH2PO4) with PEG 2000 were studied in order to understand the influence of pH and type of salt on phenols partition. Each ATPS was studied under different conditions (polymer/salt ratio, temperature, volume ratio and solid–liquid ratio). In all the ATPS studied, it was found that phenols had preference for the PEG-rich phase and it was demonstrated that the highest phenols recovery was favored by a high polymer/salt ratio, high temperature and a high volume ratio. The maximum total phenols yield in the top phase, 1.89 mg gallic acid equivalent/100 mg oven dried wood, was attained at 65 °C and a solid/liquid ratio of 0.5:10 using a PEG 2000/K2HPO4 ATPS. However, higher partition coefficients were attained for PEG 2000/K2HPO4–KH2PO4 ATPS. It was found that at low pH values higher partition of phenolic compounds was achieved. Extract analysis by RP-HPLC–ESI-TOF confirmed the presence of various phenolics compounds with antioxidant activity such as ellagic acid and quercetin 3-O-rhamnoside.
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References
Pokorný, J., Trojáková, L., Takácsová, M.: The use of natural antioxidants in food products of plant origin. In: Pokorný, J., Yanishlieva, N., Gordon, M. (eds.) Antioxidants in Food. Practical Applications, pp. 355–372. CRC Press, Boca Ratón, New York (2001)
Vázquez, G., Fernández-Agulló, A., Gómez-Castro, C., Freire, M.S., Antorrena, G., González-Álvarez, J.: Response surface optimization of antioxidans extraction from chestnut (Castanea sativa) bur. Ind. Crop. Prod. 35, 126–134 (2012)
Routray, W., Orsat, V.: MAE of phenolic compounds from blueberry leaves and comparison with other extraction methods. Ind. Crop Prod. 58, 36–45 (2014)
Jokić, S., Velić, D., Bilić, M., Bucić-Kojić, A., Planinić, M., Tomas, S.: Modelling of the process of solid-liquid extraction of total polyphenols from soybeans. Czech. J. Food Sci. 28, 206–212 (2010)
Piwowarska, N., González-Álvarez, J.: Extraction of antioxidants from forestry biomass: kinetics and optimization of extraction conditions. Biomass Bioenerg. 43, 42–51 (2012)
Zuorro, A., Lavecchia, R.: Spent coffee grounds as a valuable source of phenolic compounds and bioenergy. J. Clean. Prod. 34, 49–56 (2012)
Zuorro, A., Maffei, G., Lavecchia, R.: Reuse potential of artichoke (Cynara scolimus L.) waste for the recovery of phenolic compounds and bioenergy. J. Clean. Prod. 34, 279–284 (2016)
Albertsson, P.: Partition of Cell Particles and Macromolecules. Wiley, New York (1986)
Benavides, J., Rito-Palomares, M.: Practical experiences from the development of aqueous two-phase processes for the recovery of high value biological products. J. Chem. Technol. Biotechnol. 83, 133–142 (2008)
Goja, M.A., Yang, H., Cui, M., Li, C.: Aqueous two-phase extraction advances for bioseparation. J. Bioproces Biotechniq. 4, 1–8 (2014)
Haraguchi, L.H., Mohamed, R.S., Loh, W., Pessoa Filho, P.A.: Phase equilibrium and insulin partitioning in aqueous two-phase systems containing block copolymers and potassium phosphate. Fluid Ph. Equilibr. 215, 1–15 (2004)
Cavalcanti, M.T.H., Carneiro-da-Cunha, M.G., Brandi, I.V., Porto, T.S., Pessoa, A.: Continuous extraction of α-toxin from a fermented broth of Clostridium perfringens Type A in perforated rotating disc contactor using aqueous two-phase PEG–phosphate system. Chem. Eng. Process. Process Intensif. 47, 1771–1776 (2008)
Porto, T.S., Medeiros e Silva, G.M., Porto, C.S., Cavalcanti, M.T.H., Neto, B.B., Lima-Filho, J.L., Converti, A., Porto, A.L.F., Pessoa Jr., A.: Liquid–liquid extraction of proteases from fermented broth by PEG/citrate aqueous two-phase system. Chem. Eng. Process. Process Intensif. 47, 716–721 (2008)
Ravindra Babu, B., Rastogi, N.K., Raghavarao, K.S.M.S.: Liquid–liquid extraction of bromelain and polyphenol oxidase using aqueous two-phase system. Chem. Eng. Process. Process Intensif. 47, 83–89 (2008)
Aguilar, O., Rito-Palomares, M.: Aqueous two-phase systems strategies for the recovery and characterization of biological products from plants. J. Sci. Food Agric. 90, 1385–1392 (2010)
Hasmann, F.A., Santos, V.C., Gurpilhares, D.B., Pessoa-Junior, A., Roberto, I.C.: Aqueous two-phase extraction using thermoseparating copolymer: a new system for phenolic compounds removal from hemicelullosic hydrolysate. J. Chem. Technol. Biotechnol. 83, 167–173 (2008)
Wang, H., Dong, Y., Xiu, Z.: Microwave-assisted aqueous two-phase extraction of piceid, resveratrol and emodin from polygonum cuspidatum by ethanol/ammonium sulphate systems. Biotechnol. Lett. 30, 2079–2084 (2008)
Wu, X., Liang, L., Zou, Y., Zhao, T., Zhao, J., Li, F., Yang, L.: Aqueous two-phase extraction, identification and antioxidant activity of anthocyanins from mulberry (Morus atropurpurea Roxb.). Food Chem. 129, 443–453 (2011)
Gómez-Loredo, A., Benavides, J., Rito-Palomares, M.: Partition behavior of fucoxanthin in ethanol-potassium phosphate two-phase systems. J. Chem. Technol. Biotechnol. 89, 1637–1645 (2014)
Xavier, L., Freire, M.S., Vidal-Tato, I., González-Álvarez, J.: Aqueous two-phase systems for the extraction of phenolic compounds from eucalyptus (Eucalyptus globulus) wood industrial wastes. J. Chem. Technol. Biotechnol. 89, 1772–1778 (2014)
Xavier, L., Freire, M.S., Vidal-Tato, I., González-Álvarez, J.: Application of aqueous two phase systems based on polyethylene glycol and sodium citrate for the recovery of phenolic compounds from eucalyptus wood. Maderas-Cienc. Tecnol. 17(3), 345–354 (2015)
Rosa, P.A.J., Ferreira, I.F., Azevedo, A.M., Aires-Barros, M.R.: Aqueous two-phase systems: a viable platform in the manufacturing of biopharmaceuticals. J. Chromatogr. A 1217, 2269–2305 (2010)
Peng, Q., Li, Z., Li, Y.: Thermodynamics of potassium hydrogen phosphate-potassium dihydrogen phosphate-polyethylene glycol aqueous two-phase systems. Fluid. Ph. Equilibr. 95, 341–357 (1994)
Singleton, V.L., Rossi, J.A.: Colorimetry of total phenolics with phosphomolybdic—phosphotungstic acid reagents. Am. J. Enol. Vitic. 16, 144–158 (1965)
Rowell, M.R., Pettersen, R., Tshabalala, M.A.: Cell wall chemistry. In: Rowell, M.R. (ed.) Handbook of Wood Chemistry and Wood Composites, pp. 44–45. CRC Press, New York (2013)
Shen, S., Chang, Z., Liu, H.: Three-liquid-phase extraction systems for separation of phenol and p-nitrophenol from wastewater. Sep. Purif. Technol. 49, 217–222 (2006)
Cláudio, A.F.M., Ferreira, A.M., Freire, C.S.R., Silvestre, A.J.D., Freire, M.G., Coutinho, J.A.P.: Optimization of the gallic acid extraction using ionic-liquid-based aqueous two-phase systems. Sep. Purif. Technol. 97, 142–149 (2012)
Salic, A., Tusek, A., Fabek, D., Rukavina, I., Zelic, B.: Aqueous two-phase extraction of polyphenols using a microchannel system—process optimization and intensification. Food Technol. Biotechnol. 49, 495–501 (2011)
Gomes, G.A., Azevedo, A.M., Aires-Barros, M.R., Prazeres, D.M.F.: Purification of plasmid DNA with aqueous two phase systems of PEG 600 and sodium citrate/ammonium sulfate. Sep. Purif. Technol. 65, 22–30 (2009)
Zhang, Y., Cremer, P.S.: Interactions between macromolecules and ions: the Hofmeister series. Curr. Opin. Chem. Biol. 10, 658–663 (2006)
Reis, I.A.O., Santos, S.B., Pereira, F.D.S., Sobral, C.R.S., Freire, M.G., Freitas, L.S., Soares, C.M.F., Lima, A.S.: Extraction and recovery of rutin from acerola waste using alcohol-salt-based aqueous two-phase systems. Separ. Sci. Technol. 49, 656–663 (2014)
Voros, N., Proust, P., Fredenslund, A.: Liquid-liquid phase equilibria of aqueous two-phase systems containing salts and polyethylene glycol. Fluid Ph. Equilibr. 90, 333–353 (1993)
Radojkovic, M., Zekovic, Z., Jokic, S., Vidovic, S., Lepojevic, Z., Milocevic, S.: Optimization of solid-liquid extraction of antioxidants from black mulberry leaves by response surface methodology. Food Technol. Biotechnol. 50(2), 167–176 (2012)
Fernández-Agulló, A., Freire, M.S., González-Álvarez, J.: Effect of the extraction technique on the recovery of bioactive compounds from eucalyptus (Eucalyptus globulus) wood industrial wastes. Ind. Crops Prod. 64, 105–113 (2015)
Santos, S.A.O., Vilela, C., Freire, C., Neto, C.P., Silvestre, A.J.D.: Ultra-high performance liquid chromatography coupled to mass spectrometry applied to the identification of valuable phenolic compounds from eucalyptus wood. J. Chromatogr. B 938, 65–74 (2013)
Conde, E., Cadahia, E., Garcia Vallejo, M.C., Diez, R.: Composición polifenólica de madera, corteza y hojas de Eucalyptus camaldulensis, E. globulus y E. rudis. Invest. Agrar. Sist. Recur. For. 5, 333–350 (1996)
Eyles, A., Davies, N.W., Mohammed, C.: Wound wood formation in Eucalyptus globulus and Eucalyptus nitens: anatomy and chemistry. Can. J. For. Res. 33, 2331–2339 (2003)
Moure, A., Cruz, J.M., Franco, D., Domínguez, J.M., Sineiro, J., Domínguez, H., Núñez, M.J., Parajó, J.C.: Natural antioxidants from residual sources. Food Chem. 72, 145–171 (2001)
Romani, A., Pinelli, P., Mulinacci, N., Vincieri, F.F., Tattini, M.: Identification and quantitation of polyphenols in leaves of Myrtus communis L. Chromatographia 49(1–2), 17–20 (1999)
Narayanan, B.A., Geoffroy, O., Willingham, M.C., Re, G.G., Nixon, D.W.: p53/p21(WAF1/CIP1) expression and its possible role in G1 arrest and apoptosis in ellagic acid treated cancer cells. Cancer Lett. 136, 215–221 (1999)
Mertens-Talcott, S.U., Talcott, S.T., Percival, S.S.: Low concentrations of quercetin and ellagic acid synergistically influence proliferation, cytotoxicity and apoptosis in MOLT-4 human leukemia cells 1–3. J. Nutr. 133, 2669–2674 (2003)
Acknowledgments
The authors gratefully acknowledge the support of the company Aserpal S.A. (Curtis, A Coruña, Spain) which provided the eucalyptus wood veneer trimmings used for carrying out this research. The authors also want to thank the EMUNDUS17 project for a grant awarded to Lucía Xavier.
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Xavier, L., Freire, M.S., Vidal-Tato, I. et al. Recovery of Phenolic Compounds from Eucalyptus globulus Wood Wastes using PEG/phosphate Aqueous Two-Phase Systems. Waste Biomass Valor 8, 443–452 (2017). https://doi.org/10.1007/s12649-016-9579-0
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DOI: https://doi.org/10.1007/s12649-016-9579-0