Waste and Biomass Valorization

, Volume 7, Issue 4, pp 677–689 | Cite as

Production of Bio-Based Hydrogen Enriched Methane from Waste Glycerol in a Two Stage Continuous System

  • Athanasios S. Dounavis
  • Ioanna Ntaikou
  • Maria Kamilari
  • Gerasimos Lyberatos
Original Paper

Abstract

In the present work, a continuous process was developed aiming at the production of bio-based hydrogen-enriched methane, from waste glycerol (WG) in a two-stage reactor system. In the first step, biohydrogen production was studied, using an attached mixed acidogenic consortium in an up-flow column bioreactor. Cylindrical porous ceramic beads with a surface area of 600 m2L−1 were used as attachment matrix of bacterial cells. The hydrogen yield, the substrate consumption and the distribution of soluble metabolites were investigated for two different substrate concentrations in the feed, i.e. 20 and 25 g WG/L. SEM pictures of the biofilm formed on the ceramic beads revealed that bacilli dominated in the reactor. Subsequently, RISA methodology showed that Klebsiella sp. and Clostridium sp. were among the dominant microorganisms. In parallel, a methanogenic reactor was started up and operated in continuous mode using initially commercial glycerol, and subsequently WG as carbon sources. In the sequel, the effluent of the hydrogenic reactor was fed to the methanogenic reactor (constituting thus the second stage in a two-stage process), and the effect of organic loading on the methane yield was studied. It was shown that the reactor managed to generate up to 73 % of the theoretically expected methane based on COD removal, corresponding to 256.0 ± 2.6 L CH4/kg WG. Moreover, simulation of the experimental data of the methanogenic reactor via the Anaerobic Digestion Model ADM1 revealed that the model was able to successfully describe the performance of the digester, even under dynamic conditions.

Keywords

Waste glycerol Biohydrogen Methane 16S rRNA RISA ADM1 

Notes

Acknowledgments

The study was partially financed by GSRT in the framework of the project BIOREF, 09SYN-81-715 as well as by the project “PEFYKA”, which is implemented in the context of the Action “Development proposals of Research Organizations- KRIPIS”, funded by the Operational Programme “Competitiveness and Entrepreneurship” (OPCE-II), Priority Axis (PA) 1, “Creation and Development of Innovation Supported by Research and Technological Development” and the “Regional Operational Programmes (ROP)” in the 3 Regions of transitional support of the National Strategic Reference Framework (NSRF) 2007–2013. The Public Expenditure has been co-financed by the European Regional Development Fund (ERDF), the European Union and Greek national Funds. The authors would like to thank Dr. Stefanos Dailianis for his contribution in the statistical data analysis as well as Dr.rer.nat. Constantin Flytzanis for providing the lab equipment for the RISA methodology.

References

  1. 1.
    Hora, T.S., Agarwal, A.K.: Experimental study of the composition of hydrogen enriched compressed natural gas on engine performance, combustion and emission characteristics. Fuel 160, 470–478 (2015)CrossRefGoogle Scholar
  2. 2.
    Alavandi, S., Agrawal, A.: Experimental study of combustion of hydrogen-syngas/methane fuel mixtures in a porous burner. Int. J. Hydrog. Energy 33, 1407–1415 (2008)CrossRefGoogle Scholar
  3. 3.
    Antonopoulou, G., Gavala, H.N., Skiadas, I.V., Angelopoulos, K., Lyberatos, G.: Biofuels generation from sweet sorghum: fermentative hydrogen production and anaerobic digestion of the remaining biomass. Biores. Technol. 99, 110–119 (2008)CrossRefGoogle Scholar
  4. 4.
    Antonopoulou, G., Stamatelatou, K., Venetsaneas, N., Kornaros, M., Lyberatos, G.: Biohydrogen and methane production from cheese whey in a two-stage anaerobic process. Ind. Eng. Chem. Res. 47, 5227–5233 (2008)CrossRefGoogle Scholar
  5. 5.
    Koutrouli, H.C., Kalfas, H., Gavala, H.N., Skiadas, I.V., Stamatelatou, K., Lyberatos, G.: Hydrogen and methane production through two-stage mesophilic anaerobic digestion of olive pulp. Bioresour. Technol. 100, 3718–3723 (2009)CrossRefGoogle Scholar
  6. 6.
    Cavinato, C., Bolzonella, D., Fatone, F., Cecchi, F., Pavan, P.: Optimization of two-phase thermophilic anaerobic digestion of biowaste for hydrogen and methane production through reject water recirculation. Bioresour. Technol. 102, 8605–8611 (2011)CrossRefGoogle Scholar
  7. 7.
    Cavinato, C., Giuliano, A., Bolzonella, D., Pavan, P., Cecchi, F.: Bio-hythane production from food waste by dark fermentation coupled with anaerobic digestion process: a long term pilot scale experience. Int. J. Hydrog. Energy 37, 11549–11555 (2011)CrossRefGoogle Scholar
  8. 8.
    Massanet-Nicolau, J., Dinsdale, R., Guwy, A., Shipley, G.: Use of real time gas production data for more accurate comparison of continuous single-stage and two-stage fermentation. Bioresour. Technol. 129, 561–567 (2013)CrossRefGoogle Scholar
  9. 9.
    Ntaikou, I., Antonopoulou, G., Lyberatos, G.: Biohydrogen production from biomass and wastes via dark fermentation: a review. Waste Biomass Valor 1, 21–39 (2010)CrossRefGoogle Scholar
  10. 10.
    Murarka, A., Dharmadi, Y., Yazdani, S.S., Gonzalez, R.: Fermentative utilization of glycerol by Escherichia coli and its implications for the production of fuels and chemicals. Appl. Environ. Microbiol. 74(4), 1124–1135 (2008)CrossRefGoogle Scholar
  11. 11.
    Serrano, A., Siles, J.A., Chica, A.F., Martin, M.A.: Improvement of mesophilic anaerobic co-digestion of agri-food waste by addition of glycerol. J. Environ. Manag. 140, 76–82 (2014)CrossRefGoogle Scholar
  12. 12.
    Böttger, E.C.: Approaches for identification of microorganisms. ASM News 62, 247–250 (1996)Google Scholar
  13. 13.
    Kolbert, C.P., Persing, D.H.: Ribosomal DNA sequencing as a tool for identification of bacterial pathogens. Curr. Opin. Microbiol. 2, 299–305 (1999)CrossRefGoogle Scholar
  14. 14.
    Patel, J.B.: 16S rRNA gene sequencing for bacterial pathogen identification in the clinical laboratory. Mol. Diagn. 6, 313–321 (2001)CrossRefGoogle Scholar
  15. 15.
    Bosshard, P.P., Zbinden, R., Abels, S., Böddinghaus, B., Altwegg, M., Böttger, E.C.: 16S rRNA Gene sequencing versus the API 20 NE system and the VITEK 2 ID-GNB card for identification of nonfermenting gram-negative bacteria in the clinical laboratory. J. Clin. Microb. 44(4), 1359–1366 (2006)CrossRefGoogle Scholar
  16. 16.
    Dounavis, A.S., Ntaikou, I., Lyberatos, G.: Production of biohydrogen from crude glycerol in an upflow column bioreactor. Biores. Technol. 198, 701–708 (2015)Google Scholar
  17. 17.
    Chen, C.C., Lin, C.Y., Lin, M.C.: Acid-base enrichment enhances anaerobic hydrogen production process. Appl. Microbiol. Biotechnol. 58, 224–228 (2002)CrossRefGoogle Scholar
  18. 18.
    Owens, J.M., Chynoweth, D.P.: Biochemical methane potential of municipal solid waste (MSW) components. Water Sci. Technol. 27, 1–14 (1993)CrossRefGoogle Scholar
  19. 19.
    Skiadas, I.V., Lyberatos, G.: The periodic anaerobic baffled reactor. Water Sci. Technol. 38, 401–408 (1998)CrossRefGoogle Scholar
  20. 20.
    APHA, AWWA & WEF: Standard Methods for the Examination of Water and Wastewater. 19th ed. Washington, DC (1995)Google Scholar
  21. 21.
    Miskin, I.P., Farrimond, P., Head, I.M.: Identification of novel bacterial lineages as active members of microbial populations in a freshwater sediment using a rapid RNA extraction procedure and RT-PCR. Microbiology 145, 1977–1987 (1999)CrossRefGoogle Scholar
  22. 22.
    Wattanaphon, H.T., Ciesielski S., Pisutpaisal N.: Determining Microbial Dynamics of Polyhydroxyalkanoates—Producing Consortium in Waste Glycerol using RISA Technique. International Conference on Environmental and Computer Science IPCBEE vol. 19 (2011)Google Scholar
  23. 23.
    Altschul, S.F., Gish, W., Miller, W., Myers, E.W., Lipman, D.J.: Basic local alignment search tool. J. Mol. Biol. 215, 403–410 (1990)CrossRefGoogle Scholar
  24. 24.
    Batstone, D.J., Keller, J., Angelidaki, I., Kalyuzhnyi, S., Pavlostathis, S.G., Rozzi, A., Sanders, W., Siegrist, H., Vavilin, V.: Anaerobic Digestion Model No. 1 (ADM1) IWA Scientific and Technical Report No. 13. IWA Publishing, London (2002)Google Scholar
  25. 25.
    Vlassis, T. Valorization of glycerol via energy recovery processes: biogas, biohydrogen and/or electricity generations in microbial fuel cells. PhD thesis, University of Patras. 149–162 (2011)Google Scholar
  26. 26.
    Antonopoulou, G., Gavala, H.N., Skiadas, I.V., Lyberatos, G.: Modeling of fermentative hydrogen production from sweet sorghum extract based on modified ADM1. Int. J. Hydrog. Energy 37, 191–208 (2012)CrossRefGoogle Scholar
  27. 27.
    Antonopoulou, G., Gavala, H.N., Skiadas, I.V., Lyberatos, G.: ADM1-based modeling of methane production from acidified sweet sorghum extract in a two stage process. Biores. Technol. 106, 10–19 (2012)CrossRefGoogle Scholar
  28. 28.
    Ntaikou, I., Gavala, H.N., Lyberatos, G.: Modeling of fermentative hydrogen production from the bacterium Ruminococcus albus: definition of metabolism and kinetics during growth on glucose. Int. J. Hydrog. Energy 34, 3697–3709 (2009)CrossRefGoogle Scholar
  29. 29.
    Reichert, P.: User Manual of AQUASIM 2.0 for the Identification and Simulation of Aquatic Systems. Swiss Federal Institute for Environmental Science and Technology, Dubendorf (1998)Google Scholar
  30. 30.
    Akutsu, Y., Lee, D.-Y., Li, Y.-Y., Noike, T.: Hydrogen production potentials and fermentative characteristics of various substrates with different heat-pretreated natural microflora. Int. J. Hydrog. Energy 34, 5365–5372 (2009)CrossRefGoogle Scholar
  31. 31.
    Drozdzynska, A., Leja, K., Czaczyk, K.: Biotechnological production of 1,3-propanediol from crude glycerol. Biotechnologia 92(1), 92–100 (2011)CrossRefGoogle Scholar
  32. 32.
    Chadwick, L.J., Irgens, R.L.: Hydrogen gas production by an Ectothiorhodospira vacuolata strain. Appl. Environ. Microbiol. 57, 594–596 (1991)Google Scholar
  33. 33.
    Tsuihiji, H., Yamazaki, Y., Kamikubo, H., Imamoto, Y., Kataoka, M.: Cloning and characterization of nif structural and regulatory genes in the purple sulfur bacterium, Halorhodospira halophila. J. Biosci. Bioeng. 101, 263–270 (2006)CrossRefGoogle Scholar
  34. 34.
    Zheng, Z.-m., Guo, N.-n., Hao, J., Cheng, K.-K., Sun, Y., Liu, D.-h.: Scale-up of micro-aerobic 1,3-propanediol production with Klebsiella pneumonia CGMCC 1.6366. Process Biochem. 44, 944–948 (2009)CrossRefGoogle Scholar
  35. 35.
    Celinska, E.: Klebsiella spp as a 1,3-propanediol producer the metabolic engineering approach. Crit. Rev. Biotechnol. 32, 274–288 (2012)CrossRefGoogle Scholar
  36. 36.
    He, L., Zhao, X., Cheng, K., Sun, Y., Liu, D.: Isolation of microorganisms able to produce 1,3-propanediol and optimization of medium constituents for Klebsiella pneumoniae AJ4. App. Biochem. Biotechnol. 169, 312–326 (2013)CrossRefGoogle Scholar
  37. 37.
    Durgapal, M., Kumar, V., Yang, T.H., Lee, H.J., Seung, D., Park, S.: Production of 1,3-propanediol from glycerol using the newly isolated Klebsiella pneumoniae J2B. Biores. Technol. 159, 223–231 (2014)CrossRefGoogle Scholar
  38. 38.
    Xiao, Y., Zhang, X., Zhu, M., Tan, W.: Effect of the culture media optimization, pH and temperature on the biohydrogen production and the hydrogenase activities by Klebsiella pneumoniae ECU-15. Biores. Technol. 137, 9–17 (2013)CrossRefGoogle Scholar
  39. 39.
    Chookaew, T., O-Thong, S., Prasertsan, P.: Fermentative production of hydrogen and soluble metabolites from crude glycerol of biodiesel plant by the newly isolated thermotolerant Klebsiella pneumoniae TR17. Int. J. Hydrog. Energy 37, 13314–13322 (2012)CrossRefGoogle Scholar
  40. 40.
    Bernal, M., Tinoco, L.K., Torres, L., Malagon-Romero, D., Montoya, D.: Evaluating Colombian Clostridium spp. strains hydrogen production using glycerol as substrate. Electron. J. Biotechnol. 16. http://dx.doi.org/10.2225/vol16-issue2-fulltext-4 (2013)
  41. 41.
    Wong, Y.M., Wu, T.Y., Juan, J.C.: A review of sustainable hydrogen production using seed sludge via dark fermentation. Ren. Sust. Energy Rev. 34, 471–482 (2014)CrossRefGoogle Scholar
  42. 42.
    Baroi, G.N., Skiadas, I.V., Westermann, P., Gavala, H.N.: Continuous fermentation of wheat straw hydrolysate by Clostridium tyrobutyricum with in situ acids removal. Waste Biomass Valor. 6, 317–326 (2015)CrossRefGoogle Scholar
  43. 43.
    Yossan, S., O-Thong, S., Prasertsan, P.: Effect of initial pH, nutrients and temperature on hydrogen production from palm oil mill effluent using thermotolerant consortia and corresponding microbial communities. Int. J. Hydrog. Energy 37, 13806–13814 (2012)CrossRefGoogle Scholar
  44. 44.
    Zhang, C., Xing, X.-H.: Quantification of a specific bacterial strain in an anaerobic mixed culture for biohydrogen production by the aerobic fluorescence recovery (AFR) technique. Biochem. Eng. J. 39, 581–585 (2008)CrossRefGoogle Scholar
  45. 45.
    Rittmann, B.E., McCarty, P.L.: Environmental Biotechnology: Principles and Applications. McGraw-Hill International Edition, New York (2001)Google Scholar
  46. 46.
    Vlassis, T., Antonopoulou, G., Stamatelatou, K., Lyberatos, G.: Anaerobic treatment of glycerol for methane and hydrogen production. Glob. Nest J. 14, 149–156 (2012)Google Scholar
  47. 47.
    Vlassis, T., Stamatelatou, K., Antonopoulou, G., Lyberatos, G.: Methane production via anaerobic digestion of glycerol: a comparison of conventional (CSTR) and high-rate (PABR) digesters. J. Chem. Technol. Biotechnol. 88, 2000–2006 (2013)Google Scholar
  48. 48.
    Siles, J., Santos, M., Perez, A., Martin, A.: Anaerobic digestion of glycerol derived from biodiesel manufacturing. Biores. Technol. 100, 5609–5615 (2009)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Athanasios S. Dounavis
    • 1
    • 2
  • Ioanna Ntaikou
    • 2
  • Maria Kamilari
    • 3
  • Gerasimos Lyberatos
    • 2
    • 4
  1. 1.Department of Chemical EngineeringUniversity of PatrasPatrasGreece
  2. 2.Institute of Chemical Engineering and SciencesFoundation for Research and Technology Hellas (ICEHT/FORTH)PatrasGreece
  3. 3.Department of Biology, Division of Genetics, Cell and Developmental BiologyUniversity of PatrasPatrasGreece
  4. 4.School of Chemical EngineeringNational Technical University of AthensAthensGreece

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