Abstract
After acid hydrolysis with \(\hbox {H}_2\hbox {SO}_4\) from olive pruning, subsequent fermentations were carried out in order to compare two non-traditional yeasts: Candida guilliermondii and Pichia stipitis. During the fermentations, sugar uptake as well as ethanol and xylitol production were determined. However, both yeast employed for the biotransformations showed different behaviours; C. guilliermondii produced ethanol from D-glucose and xylitol from D-xylose but, in contrast, P. stipitis only was able to produce ethanol from hexoses and pentoses although, due to the inhibitors amount (acetic acid and polyphenols mainly), it required a detoxification step. To solve this problem, activated charcoal treatment as well as a vacuum evaporation process (concentration ratio 2.7) were performed as physical detoxification methods with positive results. The maximum ethanol and xylitol yields (\(Y_{P/S}\)) (calculated on consumed sugars) obtained with C. guilliermondii were 0.38 and 0.31 kg \(\hbox {kg}^{-1}\) respectively; while P. stipitis was able to produce 0.33 kg of ethanol per kg of fermentable sugar.
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Acknowledgments
The authors acknowledge the financial support of “Consejería de Economía, Innovación y Ciencia” from Andalusia Regional Government (Project AGR-6509) (Spain) and the Programa Ciência sem Fronteiras do Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Brazil).
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Moya, A.J., Mateo, S., Puentes, J.G. et al. Comparing Bioalcohols Production from Olive Pruning Biomass by Biotechnological Pathway with Candida guilliermondii and Pichia stipitis . Waste Biomass Valor 7, 1369–1375 (2016). https://doi.org/10.1007/s12649-016-9531-3
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DOI: https://doi.org/10.1007/s12649-016-9531-3