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Mango Peel as Source of Antioxidants and Pectin: Microwave Assisted Extraction

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Abstract

In this study, we evaluated the microwave-assisted extraction of pectin and polyphenols from mango (Mangifera indica) peels var. Ataulfo using several extraction conditions (time and temperature). Under best extraction conditions, an extract was prepared from dehydrated and pulverized mango peels and fractionated by liquid chromatography on Amberlite XAD-16, until obtain an polyphenols-rich ethanolic fraction which was evaporated to recover the total polyphenols as fine powder. Chemical and antioxidant properties were measured. The dry peels represent only 2.66 % of the fresh mango, however this extraction method was shown to significantly act upon yield of pectin (3.88–10.43 % dry weight). Also, this material is an important source of polyphenols (11.66 mg g−1 dry peels) with higher antioxidant capacity (70.31, 91.46 and 86.46 % of inhibition to DPPH, ABTS and Lipid oxidation inhibition, respectively) at 75 ppm. Based in our findings a good recovery yield was obtained. Fine characteristics of the pectin and polyphenols make them an important biomaterial that can be exploited and used in food, pharmaceutical and other industries.

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References

  1. Masmoudi, M., Besbes, S., Abbes, F., Robert, C., Paquot, M., Blecker, C., et al.: Pectin extraction from lemon by-product with acidified data juice: effect of extraction conditions on chemical composition of pectins. Food Bioprocess Technol. 5(2), 687–695 (2012)

    Article  Google Scholar 

  2. Berardini, N., Fezer, R., Conrad, J., Beifuss, U., Carle, R., Schieber, A.: Screening of mango (Mangifera indica L.) cultivars for their contents of flavonol O- and xanthone C-glycosides, anthocyanins, and pectin. J. Agric. Food Chem. 53(5), 1563–1570 (2005)

    Article  Google Scholar 

  3. Berardini, N., Knödler, M., Schieber, A., Carle, R.: Utilization of mango peels as source of pectin and polyphenolics. Innov. Food Sci. Emerg. Technol. 6(4), 442–452 (2005)

    Article  Google Scholar 

  4. Levigne, S., Ralet, M.C., Thibault, J.F.: Characterization of pectins extracted from fresh sugar beet under different conditions using an experimental design. Carbohydr. Polym. 49(2), 145–153 (2002)

    Article  Google Scholar 

  5. Pérez-Martínez, J.D., Sánchez-Becerril, M., Ornelas-Paz, J.J., González-Chávez, M.M., Ibarra-Junquera, V., Escalante-Minakata, P.: The effect of extraction conditions on the chemical characteristics of pectin from Opuntia ficus indica cladode flour. J. Polym. Environ. 21(4), 1040–1051 (2013)

    Article  Google Scholar 

  6. Robles-Sánchez, R.M., Islas-Osuna, M.A., Astiazaran-Garcia, H., Vásquez-Ortiz, F.A., Martin-Belloso, O., Goristein, S., et al.: Quality index, consumer acceptability, bioactive compounds, and antioxidant activity of fresh cut “Ataulfo” mangoes (Mangifera indica L.) as affected by low-temperatures storage. JFS S Sens. Food Qual. 4(3), 126–134 (2009)

    Google Scholar 

  7. Moalemiyan, M., Ramaswamy, H.S., Maftoonazad, N.: Pectin-based edible coating for shel life extensión of Ataulfo mango. J. Food Process Eng. 35(4), 572–600 (2011)

    Article  Google Scholar 

  8. Kansci, G., Koubala, B.B., Lape, M.I.: Effect of ripening on the composition and the suitability for jam processing of different varieties of mango (Mangifera indica). Afr. J. Biotechnol. 2(9), 301–306 (2003)

    Article  Google Scholar 

  9. Koubala, B.B., Kansci, G., Mbome, L.I., Crépeau, M.J., Thibault, J.F., Ralet, M.C.: Efect of extraction conditions on some physicochemical characteristics of pectin from “Améliorée” and “Mango” mango peels. Food Hydrocoll. 22(7), 1345–1351 (2008)

    Article  Google Scholar 

  10. Koubala, B.B., Kansci, G., Garnier, C., Thibault, J.F., Ralet, M.C.: Physicochemical properties of dietary fibres prepared from Ambarella (Spondias cytherea) and mango (Mangifera indica) peels. Food Bioprocess Technol. 6(2), 591–597 (2013)

    Article  Google Scholar 

  11. Ralet, M.C., Thibault, J.F.: Interchain heterogeneity of enzymatically deesterified lime pectins. Biomacromolecules 3(5), 917–925 (2002)

    Article  Google Scholar 

  12. Ridley, B.L., O’Neil, M.A., Mohnen, D.: Pectins: structure, biosynthesis, and oligogalacturonide-related signaling. Phyttochemistry 5(6), 929–967 (2001)

    Article  Google Scholar 

  13. Schols, H.A., Voragen, A.G.J.: Complex pectins: structure elucidation using enzymes. Prog. Biotechnol. 14, 3–19 (1996)

    Article  Google Scholar 

  14. Barrera, A.M., Ramírez, J.A., González-Cabriales, J.J., Vázquez, M.: Effect of pectins on the gelling properties of surimi from silver carp. Food Hydrocoll. 16(5), 441–447 (2002)

    Article  Google Scholar 

  15. Momin, M., Pundarikakshudu, K., Nagori, S.A.: Design and development of mixed film of pectin: ethyl cellulose for colon specific drug delivery of sennosides and triphala. Indian J. Pharm. Sci. 70(3), 338–343 (2008)

    Article  Google Scholar 

  16. Hwang, J.K.: Production of functional carbohydrates by the extrusion reactor. Food Sci. Biotechnol. 14(8), 445–449 (2001)

    Google Scholar 

  17. Rezzoug, S.A., Sannier, F., Maache-Rezzoug, Z., Allaf, K.: A thermomechanical preprocessing for pectin isolation form orange peel with optimization by response surface methodology. Int. J. Food Eng. 4(1), 1–18 (2008)

    Article  Google Scholar 

  18. Martinez-Avila, G.C.G., Wicker, L., Aguilar, C.N., Rodriguez-Herrera, R., Contreras-Esquivel, J.C.: Production of an acidic polygalacturonase from Aspergillus kawachii by solid state fermentation and their application for pectin extraction. Food Sci. Biotechnol. 18(3), 732–738 (2009)

    Google Scholar 

  19. Maran, J.P, Swathi, K., Jeevitha, P., Jayalakshmi, J., Ashvini, G.: Microwave-assisted extraction of pectic polysaccharide from waste mango peel. Carbohydr. Polym. 5(123), 67–71 (2015)

  20. AOAC: Official methods of analysis, 14th edn. Association of Official Analytical Chemists, Washington, DC (1984)

    Google Scholar 

  21. Dubois, M., Guilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F.: Colorimetric method for determination of sugars and related substances. Anal. Chem. 28(3), 350–356 (1956)

    Article  Google Scholar 

  22. Somogyi, M.: Notes on sugar determination. J. Biol. Chem. 70, 599–612 (1950)

    Google Scholar 

  23. Seeram, N., Lee, R., Hardy, M., Herber, D.: Rapid large scale purification of ellagitannins from pomegranate husk, a by-product of a commercial juice industry. Sep. Purif. Technol. 41(1), 49–55 (2005)

    Article  Google Scholar 

  24. Ascasio-Valdés, J.A., Aguilera-Carbó, A., Martínez-Hernández, J.L., Rodríguez-Herrera, R., Aguilar, C.N.: Euphorbia antisyphilitica residues as a new source of ellagic acid. Chem. Pap. 64(4), 528–532 (2010)

    Google Scholar 

  25. Ventura, J., Belmares, R., Aguilera, A., Gutiérrez, G., Rodríguez, R., Aguilar, C.N.: Fungal biodegradation of tannins from creosote bush (Larrea tridentata) and tar bush (Flourensia cernua) for gallic and ellagic acid production. Food. Technol. Biotechnol. 46(2), 213–217 (2008)

    Google Scholar 

  26. Molyneux, P.: The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol. 26(2), 211–219 (2004)

    Google Scholar 

  27. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C.: Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26(9–10), 1231–1237 (1999)

    Article  Google Scholar 

  28. Starzynska-Janiszewska, A., Stodolak, B., Jamróz, M.: Antioxidant properties of extracts from fermented and cooked seeds of Polish cultivars of Lathyrus sativus. Food Chem. 109, 285–292 (2008)

    Article  Google Scholar 

  29. Martínez-Ávila, G., Aguilera-Carbó, A., Rodríguez-Herrera, R., Aguilar, C.: Fungal enhancement of the antioxidant properties of grape waste. Ann. Microbiol. 62(3), 923–930 (2012)

    Article  Google Scholar 

  30. Fishman, M.L., Chau, H.K., Hoagland, P.D., Hotchkiss, A.T.: Microwave-assisted extraction of lime pectin. Food Hydrocoll. 20, 1170–1177 (2006)

    Article  Google Scholar 

  31. Berardini, N., Knodler, M., Schieber, A., Carle, R.: Utilization of mango peels as a source of pectin and polyphenolics. Innov. Food Sci. Emerg. Technol. 6, 442–452 (2005)

    Article  Google Scholar 

  32. Dorta, E., Lobo, M.G., Gonzalez, M.: Reutilization of mango byproducts: study of the effect of extraction solvent and temperature on their antioxidant properties. J. Food Sci. 77(1), 80–88 (2012)

    Article  Google Scholar 

  33. Sáyago-Ayerdi, S.G., Moreno-Hernández, C.L., Montalvo-González, E., Garcia-Magaña, M.L., Mata-Montes, M., Lluís-Torres, J., et al.: Mexican Ataulfo mango (Mangifera indica L.) as a source of hydrolyzable tannins. Analysis by MALDI-TOF/TOF MS. Food Res. Int. 51(1), 188–194 (2013)

    Article  Google Scholar 

  34. Sultana, B., Hussain, Z., Asif, M., Munir, A.: Investigation on the antioxidant activity of leaves, peels, stems bark and kernel of mango (Mangifera indica L.). J/Food Sci. 77(8), 849–852 (2012)

    Google Scholar 

  35. Sirisakulwat, S., Nagel, A., Sruamsiri, P., Carle, R., Neidhart, S.: Yield and quality of pectins extractable from the peels of thai mango cultivars depending on fruit ripeness. J. Agric. Food Chem. 56(22), 10727–10738 (2008)

    Article  Google Scholar 

  36. Hana, K., Jeong, Y.M., Hyeonji, K., Dong-Sun, L., Moonjae, C., Hyung-Kyoon, C., et al.: Antioxidant and antiproliferative activities of mango (Mangifera indica L.) flesh and peel. Food Chem. 121(2), 429–436 (2010)

    Article  Google Scholar 

Download references

Acknowledgments

We gratefully acknowledge the financial support received by the National Council of Science and Technology (CONACYT, México), and the technical support of the Geoscience Center (UNAM-Juriquilla, Qro., Méx.) and CoyoteFoods Biopolymer and Biotechnology (Saltillo, Coah., Méx.) for the realization of this project.

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Correspondence to Cristobal N. Aguilar.

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Rojas, R., Contreras-Esquivel, J.C., Orozco-Esquivel, M.T. et al. Mango Peel as Source of Antioxidants and Pectin: Microwave Assisted Extraction. Waste Biomass Valor 6, 1095–1102 (2015). https://doi.org/10.1007/s12649-015-9401-4

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