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Production of Biodiesel from Waste Frying Oil Using Whole Cell Biocatalysts: Optimization of Effective Factors

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Abstract

Using whole cell as a catalyst of enzymatic transesterification is a method to produce biodiesel with fewer drawbacks and at a lower cost. In the present study, biodiesel was produced by the reaction of waste frying oil with methanol in the presence of Rhizopus oryzae PTCC 5174 in both immobilized and free forms. Effect of four influencing factors including temperature, methanol to oil molar ratio, amount of biomass, and shaking speed was investigated. Response surface methodology was used to find the optimum conditions of the transesterification reaction. Each factor was set at three levels and the yield of the biodiesel was considered as the response. A quadratic equation was well-fitted with the experimental data. The optimum condition in immobilized and free forms was: 1 g microorganism for both forms, temperature of 44 and 45 °C, 5:1 and 4.7:1 methanol to oil molar ratio, and 150 and 160 rpm shaking speed, respectively.

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Acknowledgments

Authors would like to thank the research office of Sharif University of Technology for its financial support.

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Authors and Affiliations

  1. Department of Chemical and Petroleum Engineering, Sharif University of Technology, Azadi Ave., P.O. Box 11155-9465, Tehran, Iran

    Fariba Ghaderinezhad, Hamid-Reza Kariminia & Soheila Yaghmaei

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  1. Fariba Ghaderinezhad
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  2. Hamid-Reza Kariminia
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  3. Soheila Yaghmaei
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Correspondence to Hamid-Reza Kariminia.

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Ghaderinezhad, F., Kariminia, HR. & Yaghmaei, S. Production of Biodiesel from Waste Frying Oil Using Whole Cell Biocatalysts: Optimization of Effective Factors. Waste Biomass Valor 5, 947–954 (2014). https://doi.org/10.1007/s12649-014-9314-7

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