Abstract
This study attempted to explore whether miR-363-3p play a role in the isoflurane (ISO)-mediated protective effect of cardiomyocyte injury induced by hypoxia/reoxygenation (H/R). A myocardial cell injury model was established, and the different preconditioning ISO concentrations were screened and determined. The miR-363-3p level was detected by RT-qPCR. The effects of miR-363-3p on proliferation and apoptosis of H9c2 cells were detected by CCK-8 assay and flow cytometry. Myocardial injury indexes were determined by enzyme-linked immunosorbent assay (ELISA). The interaction of miR-363-3p with the 3′-UTR of the KLF2 gene was confirmed by luciferase reporter gene assay. ISO pretreatment can reduce the up-regulation of miR-363-3p after H/R injury. ISO pretreatment reduces the inhibition of cell viability and the promotion of cell apoptosis induced by H/R stimuli, while the overexpression of miR-363-3p counteracts the protective effect of ISO pretreatment. Meanwhile, ISO pretreatment also reduced the level of markers of H/R-induced myocardial injury. Moreover, luciferase reporter analysis showed that KLF2 was the downstream target gene of miR-363-3p. ISO pretreatment may alleviate H/R-induced cardiomyocyte injury by regulating miR-363-3p.
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Data Availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author upon reasonable request.
References
Borosch S, Dahmen E, Beckers C, Stoppe C, Buhl EM, Denecke B, Goetzenich A, Kraemer S (2017) Characterization of extracellular vesicles derived from cardiac cells in an in vitro model of preconditioning. J Extracell Vesicles 6(1):1390391. https://doi.org/10.1080/20013078.2017.1390391
Canale M, Marisi G, Passardi A, Scarpi E, Ulivi P (2019) Detection of a circulating MicroRNA custom panel in patients with metastatic colorectal cancer. J Vis Exp (145). https://doi.org/10.3791/58615
Gao C, Qian H, Shi Q, Zhang H (2020) MicroRNA-363-3p serves as a diagnostic biomarker of acute myocardial infarction and regulates vascular endothelial injury by targeting KLF2. Cardiovasc Diagn Ther 10(3):421–430. https://doi.org/10.21037/cdt-19-700
Ge ZD, Li Y, Qiao S, Bai X, Warltier DC, Kersten JR, Bosnjak ZJ, Liang M (2018) Failure of isoflurane cardiac preconditioning in obese type 2 diabetic mice involves aberrant regulation of microRNA-21, endothelial nitric-oxide synthase, and mitochondrial complex I. Anesthesiology 128(1):117–129. https://doi.org/10.1097/ALN.0000000000001926
Ghalehnoei H, Bagheri A, Fakhar M, Mishan MA (2020) Circulatory microRNAs: promising non-invasive prognostic and diagnostic biomarkers for parasitic infections. Eur J Clin Microbiol Infect Dis 39(3):395–402. https://doi.org/10.1007/s10096-019-03715-8
Honemann C, Hagemann O, Doll D (2013) Inhalational anaesthesia with low fresh gas flow. Indian J Anaesth 57(4):345–350. https://doi.org/10.4103/0019-5049.118569
Jamnicki-Abegg M, Weihrauch D, Pagel PS, Kersten JR, Bosnjak ZJ, Warltier DC, Bienengraeber MW (2005) Isoflurane inhibits cardiac myocyte apoptosis during oxidative and inflammatory stress by activating Akt and enhancing Bcl-2 expression. Anesthesiology 103(5):1006–1014. https://doi.org/10.1097/00000542-200511000-00015
Kersten JR (2011) Anesthetic preconditioning: an anesthesiologist's tale 1997. Anesthesiology 114 (1):162–166. https://doi.org/10.1097/ALN.0b013e3181fe4971
Kim SJ, Kim W, Woo JS, Ha SJ, Kang WY, Hwang SH, Kang DG, Lee SU, Cho SK, Im JS, Kim W (2012) Effect of myocardial protection of intracoronary adenosine and nicorandil injection in patients undergoing non-urgent percutaneous coronary intervention: a randomized controlled trial. Int J Cardiol 158(1):88–92. https://doi.org/10.1016/j.ijcard.2011.01.011
Li T, Wu W, You Z, Zhou R, Li Q, Zhu D, Li H, Xiang X, Irwin MG, Xia Z, Liu J (2012) Alternative use of isoflurane and propofol confers superior cardioprotection than using one of them alone in a dog model of cardiopulmonary bypass. Eur J Pharmacol 677(1–3):138–146. https://doi.org/10.1016/j.ejphar.2011.12.030
Liao Y, Li H, Pi Y, Li Z, Jin S (2019) Cardioprotective effect of IGF-1 against myocardial ischemia/reperfusion injury through activation of PI3K/Akt pathway in rats in vivo. J Int Med Res 47(8):3886–3897. https://doi.org/10.1177/0300060519857839
Liu HJ, Liu B (2018) Inhibition of microRNA-23 contributes to the isoflurane-mediated cardioprotection against oxidative stress. Cardiovasc Toxicol 18(5):450–458. https://doi.org/10.1007/s12012-018-9455-1
Liu H, Li G, Zhao W, Hu Y (2016) Inhibition of miR-92a may protect endothelial cells after acute myocardial infarction in rats: role of KLF2/4. Med Sci Monit 22:2451–2462. https://doi.org/10.12659/msm.897266
Manavski Y, Abel T, Hu J, Kleinlutzum D, Buchholz CJ, Belz C, Augustin HG, Boon RA, Dimmeler S (2017) Endothelial transcription factor KLF2 negatively regulates liver regeneration via induction of activin A. Proc Natl Acad Sci USA 114(15):3993–3998. https://doi.org/10.1073/pnas.1613392114
Murry CE, Jennings RB, Reimer KA (1986) Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardium. Circulation 74(5):1124–1136. https://doi.org/10.1161/01.cir.74.5.1124
Oshima Y, Sakamoto S, Yamasaki K, Mochida S, Funaki K, Moriyama N, Otsuki A, Endo R, Nakasone M, Takahashi S, Harada T, Minami Y, Inagaki Y (2016) Desflurane inhalation before ischemia increases ischemia-reperfusion-induced vascular leakage in isolated rabbit lungs. Springerplus 5(1):2031. https://doi.org/10.1186/s40064-016-3741-9
Peng RR, Liang ZG, Chen KH, Li L, Qu S, Zhu XD (2021) Nomogram based on lactate dehydrogenase-to-albumin ratio (LAR) and platelet-to-lymphocyte ratio (PLR) for predicting survival in nasopharyngeal carcinoma. J Inflamm Res 14:4019–4033. https://doi.org/10.2147/JIR.S322475
Steijlen AS, Jansen KM, Albayrak A, Verschure DO, Van Wijk DF (2018) A novel 12-lead electrocardiographic system for home use: development and usability testing. JMIR Mhealth Uhealth 6(7):e10126. https://doi.org/10.2196/10126
Uhlig C, Bluth T, Schwarz K, Deckert S, Heinrich L, De Hert S, Landoni G, Serpa Neto A, Schultz MJ, Pelosi P, Schmitt J, Gama de Abreu M (2016) Effects of volatile anesthetics on mortality and postoperative pulmonary and other complications in patients undergoing surgery: a systematic review and meta-analysis. Anesthesiology 124(6):1230–1245. https://doi.org/10.1097/ALN.0000000000001120
Xu P, Ji L, Tian S, Li F (2018) Clinical effects of tanshinone IIA sodium sulfonate combined with trimetazidine and levocarnitine in the treatment of AVMC and its effects on serum TNF-alpha, IL-18 and IL-35. Exp Ther Med 16(5):4070–4074. https://doi.org/10.3892/etm.2018.6671
Yuan J, Fu X (2021) MicroRNA-21 mediates the protective role of emulsified isoflurane against myocardial ischemia/reperfusion injury in mice by targeting SPP1. Cell Signal 86:110086. https://doi.org/10.1016/j.cellsig.2021.110086
Zhang X, Dong S, Qin Y, Bian X (2015) Protective effect of erythropoietin against myocardial injury in rats with sepsis and its underlying mechanisms. Mol Med Rep 11(5):3317–3329. https://doi.org/10.3892/mmr.2015.3155
Zhang Y, He N, Feng B, Ye H (2020) Exercise mediates heart protection via non-coding RNAs. Front Cell Dev Biol 8:182. https://doi.org/10.3389/fcell.2020.00182
Zhang B, Jiang H, Dong Z, Sun A, Ge J (2021) The critical roles of m6A modification in metabolic abnormality and cardiovascular diseases. Genes Dis 8(6):746–758. https://doi.org/10.1016/j.gendis.2020.07.011
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YHG, CBW, BQC, and YGL carried out the concepts, design, and definition of intellectual content. CBW, DML, LZ, and LYZ provided assistance for data acquisition, data analysis, and statistical analysis. All authors performed the experiment. YHG and CBW draft of the manuscript. JM revised the manuscript critically for important intellectual content. All authors have read and approved the content of the manuscript.
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12640_2022_584_MOESM1_ESM.tif
Supplementary file1: Overexpression of miR-363-3p on cell viability and apoptosis of H/R-induced H9c2 cells. (A) Transfection of miR-363-3p mimic could further increase the miR-363-3p expression in H/R group. (B) Transfection of miR-363-3p mimic further reduced the cell viability of H/R-induced cells. (C) Transfection of miR-363-3p mimic further increased the cell apoptosis of H/R-induced cells. ***P < 0.001 vs control group, ##P < 0.01, ###P < 0.001 vs HR group. (TIF 159 KB)
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Ge, Y., Wang, C., Cui, B. et al. Isoflurane Preconditioning May Attenuate Cardiomyocyte Injury Induced by Hypoxia/Reoxygenation Possibly by Regulating miR-363-3p. Neurotox Res 40, 1895–1901 (2022). https://doi.org/10.1007/s12640-022-00584-6
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DOI: https://doi.org/10.1007/s12640-022-00584-6