Abstract
The Pax6 binds to promoter sequence elements of genes involved in immunological surveillance and interacts with Iba1, p53, Ras-GAP, and Sparc in the brain of mice. The Pax6 also affects the expression pattern of genes involved in neurogenesis and neurodegeneration. However, the expression and association of Pax6 in the brain under immunologically challenged conditions are still elusive. Therefore, it has been intended to analyze the association of Pax6 in the immunity of the brain using the immune-challenged Dalton’s lymphoma (DL) mice model. The expressions of Pax6, Iba1, and Tmem119 decreased, but expressions of Ifn-γ, Tnf-α, Bdnf, and Tgf-β increased in the brain of immune-challenged mice as compared to the control. The level of co-expression of Pax6 decreased in dual positive cells with Iba1, Tmem119, Sparc, p53, Bdnf, and Tgf-β in the brain of immune-challenged mice. Binding of Pax6 to multiple sites of the promoter sequences of Bdnf and Tgf-β indicates their Pax6-associated differential expression and association with immune responsive gene. The levels of binding of Pax6 to Tmem119, Iba1, Ifn-γ, and Tnf-α got altered during the immune-challenged state as compared to control. Results provide the first evidence of the association of Pax6 in brain-specific immunity.
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Acknowledgements
The authors are thankful to DBT-ISLS-BHU and Prof. A. K. Mishra, Department of Botany, BHU for real-time PCR facilities. The authors are very thankful to Dr. Amrita Bakshi, Department of Zoology, University of Delhi, and Dr. Suchi Gupta, Stem Cell Facility, All India Institute of Medical Science, Delhi, for English editing. The authors are grateful to Merck Millipore, Mumbai, for helping us with the Amnis Imagestreak Mark II Flow cytometer instrument facility. Technical and logistic help during the analysis of samples by Mr. Vishal Mehrotra, Miss Damini Vats, and Mrs. Debjani Kundu is gratefully acknowledged.
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The Department of Science and Technology-Science and Engineering Research Board, India (EMR/2016/006913) funded the research.
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Shashank Kumar Maurya: conceptualization, methodology, investigation, software, data curation, analysis, writing — original draft preparation; Rajnikant Mishra: conceptualization, supervision, writing — reviewing and editing.
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Maurya, S.K., Mishra, R. Co-expression and Interaction of Pax6 with Genes and Proteins of Immunological Surveillance in the Brain of Mice. Neurotox Res 40, 2238–2252 (2022). https://doi.org/10.1007/s12640-022-00562-y
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DOI: https://doi.org/10.1007/s12640-022-00562-y