Abstract
Methamphetamine (MA) and 3,4-methylenedioxymethamphetamine (MDMA) are empathogen (entactogen) psychoactive designer drugs which are mainly used for recreational purposes. Both MA and MDMA are central nervous system stimulants which are classified as monoamine neurotransmitter reuptake inhibitors. They have strong cytotoxic effects on dopaminergic and serotonergic neurons. Neurotoxicities of MA and MDMA by glial activation have been shown. The present work has investigated and measured cytotoxic, necrotic and apoptotic, and autophagic effects of MA and MDMA on U-87 MG (glial) and B104-1–1 (neuronal) cell lines by janus green, ethidium bromide/acridine orange, and monodansylcadaverine/propidium iodide staining to evaluate and compare their effects on glial and neuronal cells, respectively. The results of the present work showed that: (1) MDMA induced more potent mitochondrial toxicity, stronger necrotic and autophagic effects than MA in both B104-1–1 (neuronal) and U-87 MG (glial) cell lines; (2) although MDMA induced stronger apoptotic effect than MA on U-87 MG cell line, it had equal apoptotic effect on B104-1–1 cell line with MA; and (3) MDMA induced more potent mitochondrial toxicity, stronger necrotic, apoptotic, and autophagic effects than MA in B104-1–1 cell line than U-87 MG cell line.
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This work has been supported by Iran University of Medical Sciences & Health Services (Grant no. 14776, January 19, 2020).
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1) Dr. Asieh Hosseini cultured the cell lines, wrote the Introduction and Materials & Methods of the manuscript, and plotted some graphs. 2) Mr. Seyed Mohammad-Hossein Shetab-Boushehri helped in preparation of solutions and reagents, measurement of protein concentration, photography from the cells, and plotting some graphs. 3) Dr. Seyed Vahid Shetab-Boushehri incubated the cell lines with methamphetamine and MDMA, measured cytotoxicity, necrosis, apoptosis, and autophagy, and wrote Results and Discussion of the Manuscript.
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The study was ethically approved by Iran University of Medical Sciences ethics committee and performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments (Approval ID: IR.IUMS.REC.1398.1093).
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Hosseini, A., Shetab-Boushehri, S.MH. & Shetab-Boushehri, S.V. Evaluation of Cytotoxic, Necrotic, Apoptotic, and Autophagic Effects of Methamphetamine and 3,4-Methylenedioxymethamphetamine on U-87 MG (Glial) and B104-1–1 (Neuronal) Cell Lines. Neurotox Res 40, 1499–1515 (2022). https://doi.org/10.1007/s12640-022-00543-1
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DOI: https://doi.org/10.1007/s12640-022-00543-1