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Effects of Moderate-Intensity Interval Training on Gene Expression and Antioxidant Status in the Hippocampus of Methamphetamine-Dependent Rats

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Abstract

Methamphetamine (METH) can cause neurotoxicity and increase the risk of neurodegenerative disorders such as Alzheimer’s and Parkinson’s diseases. This study aimed to investigate the effect of moderate-intensity interval training (MIIT) on gene expression and antioxidant status of the hippocampus of METH-dependent rats. Thirty-two male Wistar rats were randomly divided into four equal groups (n = 8): saline, METH, MIIT, and METH + MIIT. METH was injected intraperitoneally at 5 mg/kg for 21 days. The MIIT(interval running) was performed on the treadmill 5 days a week for 8 weeks. Morris water maze test was performed to measure learning and memory. Then, the hippocampal tissue was extracted to evaluate changes in gene expression and biochemical enzymes. The data were analyzed using one-way and two-way ANOVA methods at p < 0.05. The results showed that METH injection significantly reduced spatial memory and antioxidant enzymes and increased the expression of α-synuclein (α-syn), cyclin-dependent kinase 5 (CDK5), tau, and phosphorylated tau (p-tau) genes compared to the saline group. MIIT significantly increased spatial memory and antioxidant enzymes. However, it reduced α-syn, CDK5, tau, and p-tau expression. Thus, this study depicted that methamphetamine-dependent rats with memory deficits have lower antioxidant enzyme levels and higher expression of α-syn, CDK5, tau, and p-tau genes, and that an 8-week MIIT may have beneficial effects on the memory impairments as well as antioxidant status and gene expression in male rats.

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Acknowledgements

The researchers express their gratitude to the staff of Neuroscience Research Center of Kerman University of Medical Sciences (KMUS).

Funding

Kerman University of Medical Sciences (KUMS). Neuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences (KUMS).

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Author notes

  1. Ahad Shafiei, Amir Hossein Haghighi & Roya Askari

    Present address: Department of Exercise Physiology, Faculty of Sport Sciences, Hakim Sabzevari University, Sabzevar, Iran

  2. Alireza Keyhani

    Present address: Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran

  3. Majid Asadi-Shekaari

    Present address: Neuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Kerman, Iran

Authors and Affiliations

  1. General Administration of Sport and Youth Kerman, Kerman, Iran

    Mahla Sadat Nabavizadeh

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  1. Ahad Shafiei
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  2. Amir Hossein Haghighi
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  3. Roya Askari
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Correspondence to Majid Asadi-Shekaari.

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Shafiei, A., Haghighi, A.H., Askari, R. et al. Effects of Moderate-Intensity Interval Training on Gene Expression and Antioxidant Status in the Hippocampus of Methamphetamine-Dependent Rats. Neurotox Res 40, 1455–1463 (2022). https://doi.org/10.1007/s12640-022-00532-4

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  • DOI: https://doi.org/10.1007/s12640-022-00532-4

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