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High Ethanol and Acetaldehyde Inhibit Glutamatergic Transmission in the Hippocampus of Aldh2-Knockout and C57BL/6N Mice: an In Vivo and Ex Vivo Analysis

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Abstract

We aimed to investigate whether ethanol (EtOH) and acetaldehyde (AcH) can affect glutamate and its receptors GluN1 and GluA1 in the hippocampus of Aldh2-knockout (Aldh2-KO) and C57BL/6N (wild-type (WT)) mice. To do this, we first examined the effect of local administration of EtOH (100 mM, 200 mM, and 500 mM) and AcH (100 μM, 200 μM, and 500 μM) on extracellular glutamate levels in freely moving mice. Retrodialysis of 200 mM and 500 mM EtOH into the hippocampus of WT and Aldh2-KO mice produced significant decreases in extracellular glutamate levels (p < 0.05). A dose of 500 mM EtOH induced a greater decrease in Aldh2-KO mice (p < 0.05) than in WT mice, indicating the action of AcH. Similarly, perfusion of 200 μM and 500 μM AcH decreased glutamate in Aldh2-KO mice (p < 0.05), but this decrease was not seen in WT mice at any AcH dose. Second, we tested whether the EtOH- and AcH-induced decrease in glutamate was associated with decreases in GluN1 and GluA1 expression, as measured by real-time PCR and Western blot. We found a significant decrease in GluN1 (p < 0.05) and GluA1 (p < 0.05) subunits after a high dose of EtOH (4.0 g/kg) and AcH (200 mg/kg) in WT mice. However, a 2.0 g/kg dose of EtOH did not produce a consistent decrease in GluN1 or GluA1 between messenger RNA and protein. In Aldh2-KO mice, all three doses of EtOH (1.0 g/kg, 2.0 g/kg, and 4.0 g/kg) and AcH (50 mg/kg, 100 mg/kg, and 200 mg/kg) decreased GluN1 expression (p < 0.05), while moderate-to-high doses of EtOH (2.0 g/kg and 4.0 g/kg) and AcH (100 mg/kg and 200 mg/kg) decreased GluA1 expression (p < 0.05). Together, these in vivo and ex vivo data suggest that EtOH and AcH decrease extracellular glutamate in the hippocampus of mice with a concomitant decrease in GluN1 and GluA1 subunits, but these effects require relatively high concentrations and may, therefore, explain the consequences of EtOH intoxication.

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References

  • Al-Rejaie S, Dar MS (2006) Behavioral interaction between nicotine and ethanol: possible modulation by mouse cerebellar glutamate. Alcohol Clin Exp Res 30:1223–1233

    PubMed  Google Scholar 

  • Ariwodola OJ, Weiner JL (2004) Ethanol potentiation of GABAergic synaptic transmission may be self-limiting: role of presynaptic GABA(B) receptors. J Neurosci 24:10679–10686

    CAS  PubMed  PubMed Central  Google Scholar 

  • Bäckström P, Hyytiä P (2004) Ionotropic glutamate receptor antagonists modulate cue-induced reinstatement of ethanol-seeking behavior. Alcohol Clin Exp Res 28:558–565

    PubMed  Google Scholar 

  • Carboni S, Isola R, Gessa GL, Rossetti ZL (1993) Ethanol prevents the glutamate release induced by N-methyl-D-aspartate in the rat striatum. Neurosci Lett 152:133–136

    CAS  PubMed  Google Scholar 

  • Cha YM, Li Q, Wilson WA, Swartzwelder HS (2006) Sedative and GABAergic effects of ethanol on male and female rats. Alcohol Clin Exp Res 30:113–118

    CAS  PubMed  Google Scholar 

  • Choi DW (1988) Glutamate neurotoxicity and diseases of the nervous system. Neuron 1:623–634

    CAS  PubMed  Google Scholar 

  • Chu B, Anantharam V, Treistman SN (1995) Ethanol inhibition of recombinant heteromeric NMDA channels in the presence and absence of modulators. J Neurochem 65:140–148

    CAS  PubMed  Google Scholar 

  • Cortese BM, Phan KL (2005) The role of glutamate in anxiety and related disorders. CNS Spectr 10:820–830

    PubMed  Google Scholar 

  • Dildy JE, Leslie SW (1989) Ethanol inhibits NMDA-induced increases in free intracellular Ca2+ in dissociated brain cells. Brain Res 499:383–387

    CAS  PubMed  Google Scholar 

  • Ding ZM, Engleman EA, Rodd ZA, McBride WJ (2012) Ethanol increases glutamate neurotransmission in the posterior ventral tegmental area of female Wistar rats. Alcohol Clin Exp Res 36:633–640

    CAS  PubMed  Google Scholar 

  • Doble A (1999) The role of excitotoxicity in neurodegenerative disease: implications for therapy. Pharmacol Ther 81:163–221

    CAS  PubMed  Google Scholar 

  • Ericson M, Molander A, Löf E, Engel JA, Söderpalm B (2003) Ethanol elevates accumbal dopamine levels via indirect activation of ventral tegmental nicotinic acetylcholine receptors. Eur J Pharmacol 467:85–93

    CAS  PubMed  Google Scholar 

  • Faingold CL, N’Gouemo P, Riaz A (1998) Ethanol and neurotransmitter interactions—from molecular to integrative effects. Prog Neurobiol 55:509–535

    CAS  PubMed  Google Scholar 

  • Fischer W, Franke H, Illes P (2003) Effects of acute ethanol on the Ca2+ response to AMPA in cultured rat cortical GABAergic nonpyramidal neurons. Alcohol Alcohol 38(5):394–399

    CAS  PubMed  Google Scholar 

  • Grant KA, Lovinger DM (1995) Cellular and behavioral neurobiology of alcohol: receptor-mediated neuronal processes. Clin Neurosci 3(3):155–164

    CAS  PubMed  Google Scholar 

  • Harris RA (1999) Ethanol actions on multiple ion channels: which are important? Alcohol Clin Exp Res 23:1563–1570

    CAS  PubMed  Google Scholar 

  • Harrison NL, Skelly MJ, Grosserode EK, Lowes DC, Zeric T, Phister S, Salling MC (2017) Effects of acute alcohol on excitability in the CNS. Neuropharmacology 122:36–45

    CAS  PubMed  PubMed Central  Google Scholar 

  • Hicklin TR, Wu PH, Radcliffe RA, Freund RK, Goebel-Goody SM, Correa PR, Proctor WR, Lombroso PJ, Browning MD (2011) Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. PNAS 108:6650–6655

    CAS  PubMed  Google Scholar 

  • Hinoi E, Takarada T, Ueshima T, Tsuchihashi Y, Yoneda Y (2004) Glutamate signaling in peripheral tissues. Eur J Biochem 271:1–13

    CAS  PubMed  Google Scholar 

  • Hoffman PL, Rabe CS, Moses F, Tabakoff B (1989) N-methyl-D-aspartate receptors and ethanol: inhibition of calcium flux and cyclic GMP production. J Neurochem 52:61937–61940

    Google Scholar 

  • Izumi Y, Nagashima K, Murayama K, Zorumski CF (2005) Acute effects of ethanol on hippocampal long-term potentiation and long-term depression are mediated by different mechanisms. Neuroscience 136(2):509–517

    CAS  PubMed  Google Scholar 

  • Jamal M, Ameno K, Uekita I, Kumihashi M, Wang W, Ijiri I (2007) Catalase mediates acetaldehyde formation in the striatum of free-moving rats. Neurotoxicology 28:1245–1248

    CAS  PubMed  Google Scholar 

  • Jamal M, Ameno K, Miki T, Wang W, Kumihashi M, Isse T, Kawamoto T, Kitagawa K, Nakayama K, Ijiri I, Kinoshita H (2009) Cholinergic alterations following alcohol exposure in the frontal cortex of Aldh2-deficient mice models. Brain Res 1295:37–43

    CAS  PubMed  Google Scholar 

  • Jamal M, Ameno K, Ruby M, Miki T, Tanaka N, Nakamura Y, Kinoshita H (2013) Ethanol- and acetaldehyde-induced cholinergic imbalance in the hippocampus of Aldh2-knockout mice does not affect nerve growth factor or brain-derived neurotrophic factor. Brain Res 1539:41–47

    CAS  PubMed  Google Scholar 

  • Jamal M, Ameno K, Miki T, Tanaka N, Ito A, Ono J, Takakura A, Kumihashi M, Kinoshita H (2016a) Ethanol and acetaldehyde differentially alter extracellular dopamine and serotonin in Aldh2-knockout mouse dorsal striatum: a reverse microdialysis study. Neurotoxicology 52:204–209

    CAS  PubMed  Google Scholar 

  • Jamal M, Ameno K, Tanaka N, Ito A, Takakura A, Kumihashi M, Kinoshita H (2016b) Ethanol and acetaldehyde after intraperitoneal administration to Aldh2-knockout mice-reflection in blood and brain levels. Neurochem Res 41:1029–1034

    CAS  PubMed  Google Scholar 

  • Jin C, Woodward JJ (2006) Effects of 8 different NR1 splice variants on the ethanol inhibition of recombinant NMDA receptors. Alcohol Clin Exp Res 30:673–679

    CAS  PubMed  Google Scholar 

  • Kanai Y, Hediger MA (2003) The glutamate and neutral amino acid transporter family: physiological and pharmacological implications. Eur J Pharmacol 479:237–247 Review

    CAS  PubMed  Google Scholar 

  • Khakpai F, Zarrindast MR, Nasehi M, Haeri-Rohani A, Eidi A (2013) The role of glutamatergic pathway between septum and hippocampus in the memory formation. EXCLI J 12:41–51

    PubMed  PubMed Central  Google Scholar 

  • Kirihara Y, Takechi M, Kurosaki K, Kobayashi Y, Kurosawa T (2013) Anesthetic effects of a mixture of medetomidine, midazolam and butorphanol in two strains of mice. Exp Anim 62:173–180

    CAS  PubMed  PubMed Central  Google Scholar 

  • Kitagawa K, Kawamoto T, Kunugita N, Tsukiyama T, Okamoto K, Yoshida A, Nakayama K, Nakayama K (2000) Aldehyde dehydrogenase (ALDH) 2 associates with oxidation of methoxyacetaldehyde; in vitro analysis with liver subcellular fraction derived from human and Aldh2 gene targeting mouse. FEBS Lett 476:306–311

    CAS  PubMed  Google Scholar 

  • Krystal JH, Petrakis IL, Mason G, Trevisan L, D’Souza DC (2003) N-methyl-D-aspartate glutamate receptors and alcoholism: reward, dependence, treatment, and vulnerability. Pharmacol Ther 99:79–94

    CAS  PubMed  Google Scholar 

  • Lau CG, Takeuchi K, Rodenas-Ruano A, Takayasu Y, Murphy J, Bennett MV, Zukin RS (2009) Regulation of NMDA receptor Ca2+ signalling and synaptic plasticity. Biochem Soc Trans 37(Pt 6):1369–1374

    CAS  PubMed  PubMed Central  Google Scholar 

  • Lima-Landman MT, Albuquerque EX (1989) Ethanol potentiates and blocks NMDA-activated single-channel currents in rat hippocampal pyramidal cells. FEBS Lett 247(1):61–67

    CAS  PubMed  Google Scholar 

  • Lof E, Chau PP, Stomberg R, Soderpalm B (2007) Ethanol-induced dopamine elevation in the rat—modulatory effects by subchronic treatment with nicotinic drugs. Eur J Pharmacol 555:139–147

    PubMed  Google Scholar 

  • Lovinger DM (1993) High ethanol sensitivity of recombinant AMPA-type glutamate receptors expressed in mammalian cells. Neurosci Lett 159:83–87

    CAS  PubMed  Google Scholar 

  • Lovinger DM, Roberto M (2013) Synaptic effects induced by alcohol. Curr Top Behav Neurosci 13:31–86

    CAS  PubMed  PubMed Central  Google Scholar 

  • Lovinger DM, White G, Weight FF (1989) Ethanol inhibits NMDA-activated ion current in hippocampal neurons. Science 243:1721–1724

    CAS  PubMed  Google Scholar 

  • Lovinger DM, White G, Weight FF (1990) NMDA receptor-mediated synaptic excitation selectively inhibited by ethanol in hippocampal slice from adult rat. J Neurosci 10(4):1372–1379

    CAS  PubMed  PubMed Central  Google Scholar 

  • Malenka RC, Nicoll RA (1999) Long-term potentiation—a decade of progress? Science 285:1870–1874

    CAS  PubMed  Google Scholar 

  • Meldrum BS (2000) Glutamate as a neurotransmitter in the brain: review of physiology and pathology. J Nutr 130(4S Suppl):1007S–1015S Review

    CAS  PubMed  Google Scholar 

  • Miladinovic T, Nashed MG, Singh G (2015) Overview of glutamatergic dysregulation in central pathologies. Biomolecules 5:3112–3141

    CAS  PubMed  PubMed Central  Google Scholar 

  • Mizoi Y, Tatsuno Y, Adachi J, Kogame M, Fukunaga T, Fujiwara S, Hishida S, Ijiri I (1983) Alcohol sensitivity related to polymorphism of alcohol-metabolizing enzymes in Japanese. Pharmacol Biochem Behav 18(Suppl 1):127–133

    PubMed  Google Scholar 

  • Moghaddam B, Bolinao ML (1994) Biphasic effect of ethanol on extracellular accumulation of glutamate in the hippocampus and the nucleus accumbens. Neurosci Lett 178:99–102

    CAS  PubMed  Google Scholar 

  • Möykkynen T, Korpi ER (2012) Acute effects of ethanol on glutamate receptors. Basic Clin Pharmacol Toxicol 111:4–13 Review

    PubMed  Google Scholar 

  • Muggironi G, Fois GR, Diana M (2013) Ethanol-derived acetaldehyde: pleasure and pain of alcohol mechanism of action. Front Behav Neurosci 7:87

    CAS  PubMed  PubMed Central  Google Scholar 

  • Nie Z, Madamba SG, Siggins GR (1994) Ethanol inhibits glutamatergic neurotransmission in nucleus accumbens neurons by multiple mechanisms. J Pharmacol Exp Ther 271:1566–1573

    CAS  PubMed  Google Scholar 

  • Novitskaya YA, Dravolina OA, Zvartau EE, Danysz W, Bespalov AY (2010) Interaction of blockers of ionotropic NMDA receptors and metabotropic glutamate receptors in a working memory test in rats. Neurosci Behav Physiol 40(7):807–811

    CAS  PubMed  Google Scholar 

  • Paxinos G, Franklin KBJ (2001) The Mouse Brain in Stereotaxic Coordinates, Seconded., Academic Press, San Diego, California

  • Piepponen TP, Kiianmaa K, Ahtee L (2002) Effects of ethanol on the accumbal output of dopamine, GABA and glutamate in alcohol-tolerant and alcohol-nontolerant rats. Pharmacol Biochem Behav 74:21–30

    CAS  PubMed  Google Scholar 

  • Quertemont E, Didone V (2006) Role of acetaldehyde in mediating the pharmacological and behavioral effects of alcohol. Alcohol Res Health 29:258–265 Review

    PubMed  PubMed Central  Google Scholar 

  • Quertemont E, Tambour S (2004) Is ethanol a pro-drug? The role of acetaldehyde in the central effects of ethanol. Trends Pharmacol Sci 25:130–134

    CAS  PubMed  Google Scholar 

  • Quertemont E, Linotte S, de Witte P (2002) Differential taurine responsiveness to ethanol in high- and low-alcohol sensitive rats: a brain microdialysis study. Eur J Pharmacol 444:143–150

    CAS  PubMed  Google Scholar 

  • Rawat AK (1974) Brain levels and turnover rates of presumptive neurotransmitters as influenced by administration and withdrawal of ethanol in mice. J Neurochem 22:915–922

    CAS  PubMed  Google Scholar 

  • Reynolds JD, Brien JF (1994) Effects of acute ethanol exposure on glutamate release in the hippocampus of the fetal and adult guinea pig. Alcohol 11:259–267

    CAS  PubMed  Google Scholar 

  • Roberto M, Madamba SG, Stouffer DG, Parsons LH, Siggins GR (2004a) Increased GABA release in the central amygdala of ethanol-dependent rats. J Neurosci 45:10159–10166

    Google Scholar 

  • Roberto M, Schweitzer P, Madamba SG, Stouffer DG, Parsons LH, Siggins GR (2004b) Acute and chronic ethanol alter glutamatergic transmission in rat central amygdala: an in vitro and in vivo analysis. J Neurosci 24(7):1594–1603

    CAS  PubMed  PubMed Central  Google Scholar 

  • Ronald KM, Mirshahi T, Woodward JJ (2001) Ethanol inhibition of N-methyl-D-aspartate receptors is reduced by site-directed mutagenesis of a transmembrane domain phenylalanine residue. J Biol Chem 276:44729–44735

    CAS  PubMed  Google Scholar 

  • Sanacora G, Treccani G, Popoli M (2012) Towards a glutamate hypothesis of depression: an emerging frontier of neuropsychopharmacology for mood disorders. Neuropharmacology 62:63–77

    CAS  PubMed  Google Scholar 

  • Sanchis-Segura C, Borchardt T, Vengeliene V, Zghoul T, Bachteler D, Gass P, Sprengel R, Spanagel R (2006) Involvement of the AMPA receptor GluR-C subunit in alcohol-seeking behavior and relapse. J Neurosci 26:1231–1238

    CAS  PubMed  PubMed Central  Google Scholar 

  • Schummers J, Browning MD (2001) Evidence for a role for GABA(A) and NMDA receptors in ethanol inhibition of long-term potentiation. Brain Res 94(1–2):9–14

    CAS  Google Scholar 

  • Shimizu K, Matsubara K, Uezono T, Kimura K, Shiono H (1998) Reduced dorsal hippocampal glutamate release significantly correlates with the spatial memory deficits produced by benzodiazepines and ethanol. Neuroscience 83:701–706

    CAS  PubMed  Google Scholar 

  • Suzdak PD, Paul SM (1987) Ethanol stimulates GABA receptor-mediated Cl- ion flux in vitro: possible relationship to the anxiolytic and intoxicating actions of alcohol. Psychopharmacol Bull 23:445–451

    CAS  PubMed  Google Scholar 

  • Tabakoff B, Hoffman PL (1993) Ethanol, sedative hypnotics, and glutamate receptor function in brain and cultured cells. Behav Genet 23:231–236 Review

    CAS  PubMed  Google Scholar 

  • Tamminga CA, Southcott S, Sacco C, Wagner AD, Ghose S (2012) Glutamate dysfunction in hippocampus: relevance of dentate gyrus and CA3 signaling. Schizophr Bull 38:927–935

    PubMed  PubMed Central  Google Scholar 

  • Theile JW, Morikawa H, Gonzales RA, Morrisett RA (2008) Ethanol enhances GABAergic transmission onto dopamine neurons in the ventral tegmental area of the rat. Alcohol Clin Exp Res 32:1040–1048

    CAS  PubMed  PubMed Central  Google Scholar 

  • Tiwari V, Veeraiah P, Subramaniam V, Patel AB (2014) Differential effects of ethanol on regional glutamatergic and GABAergic neurotransmitter pathways in mouse brain. J Neurochem 128:628–640

    CAS  PubMed  Google Scholar 

  • Tsai G, Coyle JT (2002) Glutamatergic mechanisms in schizophrenia. Annu Rev Pharmacol Toxicol 42:165–179 Review

    CAS  PubMed  Google Scholar 

  • Wall TL, Horn SM, Johnson ML, Smith TL, Carr LG (2000) Hangover symptoms in Asian Americans with variations in the aldehyde dehydrogenase (ALDH2) gene. J Stud Alcohol 61:13–17

    CAS  PubMed  Google Scholar 

  • Wang R, Reddy PH (2017) Role of glutamate and NMDA receptors in Alzheimer’s disease. J Alzheimers Dis 57:1041–1048

    CAS  PubMed  PubMed Central  Google Scholar 

  • Weiner JL, Dunwiddie TV, Valenzuela CF (1999) Ethanol inhibition of synaptically evoked kainate responses in rat hippocampal CA3 pyramidal neurons. Mol Pharmacol 56(1):85–90

    CAS  PubMed  Google Scholar 

  • Wirkner K, Eberts C, Poelchen W, Allgaier C, Illes P (2000) Mechanism of inhibition by ethanol of NMDA and AMPA receptor channel functions in cultured rat cortical neurons. Naunyn Schmiedeberg's Arch Pharmacol 362(6):568–576

    CAS  Google Scholar 

  • Xu H, Zhang Y, Ren J (2019) ALDH2 and stroke: a systematic review of the evidence. Adv Exp Med Biol 1193:195–210

    PubMed  Google Scholar 

  • Yan QS, Reith ME, Ya SG, Jobe PC (1998) Effect of systemic ethanol on basal and stimulated glutamate releases in the nucleus accumbens of freely moving Sprague-Dawley rats: a microdialysis study. Neurosci Lett 258:29–32

    CAS  PubMed  Google Scholar 

  • Yang M, Zhang Y, Ren J (2019) ALDH2 polymorphism and ethanol consumption: a genetic-environmental interaction in carcinogenesis. Adv Exp Med Biol 1193:229–236 Review

    PubMed  Google Scholar 

  • Zenki KC, Mussulini BH, Rico EP, de Oliveira DL, Rosemberg DB (2014) Effects of ethanol and acetaldehyde in zebrafish brain structures: an in vitro approach on glutamate uptake and on toxicity-related parameters. Toxicol in Vitro 28(5):822–828

    CAS  PubMed  Google Scholar 

  • Zimatkin SM, Buben AL (2007) Ethanol oxidation in the living brain. Alcohol Alcohol 42(6):529–532

    CAS  PubMed  Google Scholar 

  • Zorumski CF, Mennerick S, Izumi Y (2014) Acute and chronic effects of ethanol on learning-related synaptic plasticity. Alcohol 48:1–17

    CAS  PubMed  Google Scholar 

  • Zuo GC, Yang JY, Hao Y, Dong YX, Wu CF (2007) Ethanol and acetaldehyde induce similar changes in extracellular levels of glutamate, taurine and GABA in rat anterior cingulate cortex. Toxicol Lett 169:253–258

    CAS  PubMed  Google Scholar 

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Acknowledgments

We thank Toyoshi Isse, Toshihiro Kawamoto (University of Occupational and Environmental Health, Fukuoka, Japan), and Kyoko Kitagawa (Hamamatsu University School of Medicine, Japan) for providing the breeding pairs of Aldh2-KO mice.

Funding

This work was supported in part by a Grant-in-Aid for Scientific Research (Grant Nos. (c) 22590636 and 20590681) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

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  1. Department of Forensic Medicine, Faculty of Medicine, Kagawa University, 1750-1, Ikenobe, Miki, Kita, Kagawa, 761-0793, Japan

    Mostofa Jamal, Asuka Ito, Naoko Tanaka, Kiyoshi Ameno & Hiroshi Kinoshita

  2. Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Miki, Kagawa, Japan

    Takanori Miki

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  1. Mostofa Jamal
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Jamal, M., Ito, A., Tanaka, N. et al. High Ethanol and Acetaldehyde Inhibit Glutamatergic Transmission in the Hippocampus of Aldh2-Knockout and C57BL/6N Mice: an In Vivo and Ex Vivo Analysis. Neurotox Res 37, 702–713 (2020). https://doi.org/10.1007/s12640-020-00180-6

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