Abstract
Attractive due to an alleged high biocompatibility, silica nanoparticles have been widely used in the field of nanomedicine; however, their proven capacity to induce the synthesis and release of pro-inflammatory cytokines in several cellular models has raised concern about their safety. Glutamate, the main excitatory amino acid transmitter triggers a wide variety of signal transduction cascades that regulate protein synthesis at transcriptional and translational levels. A stimulus-dependent dynamic change in the protein repertoire in neurons and glia cells is the molecular framework of higher brain functions. Within the cerebellum, Bergmann glia cells are the most abundant non-neuronal cells and span the entire molecular layer of the cerebellar cortex, wrapping the synapses in this structure. Taking into consideration the functional role of Bergmann glia in terms of the recycling of glutamate, lactate supply to neurons, and prevention of neurotoxic insults, we decided to investigate the possibility that silica nanoparticles affect Bergmann glia and by these means alter the major excitatory neurotransmitter system in the brain. To this end, we exposed cultured chick cerebellar Bergmann glia cells to silica nanoparticles and measured [35S]-methionine incorporation into newly synthesized polypeptides. Our results demonstrate that exposure of the cultured cells to silica nanoparticles exerts a time- and dose-dependent modulation of protein synthesis. Furthermore, altered patterns of eukaryotic initiation factor 2 alpha and eukaryotic elongation factor 2 phosphorylation were present upon nanoparticle exposure. These results demonstrate that glia cells respond to the presence of this nanomaterial modifying their proteome, presumably in an effort to overcome any plausible neurotoxic effect.
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The technical assistance of Luis Cid and Blanca Ibarra is acknowledged.
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This work was supported by grants from Consejo Nacional de Ciencia y Tecnología (Conacyt-México 255087) and Soluciones para un México Verde, S.A. to A.O. A.G.R.C. was supported by a Conacyt-México fellowship (no. 423462).
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Rodríguez-Campuzano, A.G., Hernández-Kelly, L.C. & Ortega, A. Acute Exposure to SiO2 Nanoparticles Affects Protein Synthesis in Bergmann Glia Cells. Neurotox Res 37, 366–379 (2020). https://doi.org/10.1007/s12640-019-00084-0
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DOI: https://doi.org/10.1007/s12640-019-00084-0